DNA vaccination via RALA nanoparticles in a microneedle delivery system induces a potent immune response against the endogenous prostate cancer stem cell antigen

Cole, Grace; Ali, Ahlam; McErlean, Emma; Mulholland, Eoghan J.; Short, Amy; McCrudden, Cian M.; McCaffrey, Joanne; Robson, Tracy; Kett, Vicky L.; Coulter, Jonathan A.; Dunne, Nicholas; Donnelly, Ryan F.; McCarthy, Helen

doi:10.1016/j.actbio.2019.07.003

Cited by 83 publications

(43 citation statements)

References 40 publications

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“… ( Koh et al, 2018 ) DNA vaccine against antigens of prostate cancer stem cells Polyvinylpyrrolidone (PVP) Dissolving MNs (including cationic nanoparticles) Moulding – Vaccine loaded in cationic nanoparticle and integrated in polymer MNs is effectively triggered immune response towards endogenous prostate cancer and demonstrated anti-tumour activity in both prophylactic and therapeutic. ( Cole et al, 2019 ) Anti-vascular endothelial growth factor (VEGF) aptamer Polyvinyl alcohol (PVA) and polyvinylpyrrolidone (PVP) Dissolving MNs Moulding Pyramidal shape consisted of 100 microneedle array (10 × 10) with a 600 μm, height, 200 μm, base, andb500 μm, tip-to-tip distance. Polymer MNs loaded with aptamer shows high mechanical strength and immediately released to inhibit protein-mediated cell growth.…”

Section: Transdermal Drug Delivery (Tdd)mentioning

confidence: 99%

Current trends in polymer microneedle for transdermal drug delivery

AL-Japairai

Mahmood

Almurisi

et al. 2020

International Journal of Pharmaceutics

232

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Section: Transdermal Drug Delivery (Tdd)mentioning

confidence: 99%

Current trends in polymer microneedle for transdermal drug delivery

AL-Japairai

Mahmood

Almurisi

et al. 2020

International Journal of Pharmaceutics

232

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“…These vectors, however, are met with numerous trepidations as they can result in mutagenesis and have a restricted capacity for genetic material (Mingozzi and High, 2013). Non-viral options for gene therapy also exist and include cationic polymers (Olden et al, 2018), liposomes (Balazs and Godbey, 2011), and peptides (McCarthy et al, 2014;Cole et al, 2019). Indeed, recent literature published by Mulholland et al explored the delivery of an siRNA complexed with a cell penetrating peptide termed RALA from an electrospun bilayer wound patch.…”

Section: Discussionmentioning

confidence: 99%

Electrospun Biomaterials in the Treatment and Prevention of Scars in Skin Wound Healing

Mulholland

2020

Front. Bioeng. Biotechnol.

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Electrospinning is a promising method for the rapid and cost-effective production of nanofibers from a wide variety of polymers given the high surface area morphology of these nanofibers, they make excellent wound dressings, and so have significant potential in the prevention and treatment of scars. Wound healing and the resulting scar formation are exceptionally well-characterized on a molecular and cellular level. Despite this, novel effective anti-scarring treatments which exploit this knowledge are still clinically absent. As the process of electrospinning can produce fibers from a variety of polymers, the treatment avenues for scars are vast, with therapeutic potential in choice of polymers, drug incorporation, and cell-seeded scaffolds. It is essential to show the new advances in this field; thus, this review will investigate the molecular processes of wound healing and scar tissue formation, the process of electrospinning, and examine how electrospun biomaterials can be utilized and adapted to wound repair in the hope of reducing scar tissue formation and conferring an enhanced tensile strength of the skin. Future directions of the research will explore potential novel electrospun treatments, such as gene therapies, as targets for enhanced tissue repair applications. With this class of biomaterial gaining such momentum and having such promise, it is necessary to refine our understanding of its process to be able to combine this technology with cutting-edge therapies to relieve the burden scars place on world healthcare systems.

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“…The representative plasmid DNA was complexed with a cell penetrating peptide (CPP), RALA, to create stable NPs. Synthesis and characterization of RALA/pEGFP-N1 NPs are well described in the literature [29,92]. The RALA/pEGFP-N1 NPs are electrostatic; therefore, it is imperative that the delivery system is neutral, as the net charge density on hydrogel can result in unwanted interactions between delivery system and the NPs as the electrostatic forces affect the interactions between NPs and hydrogel matrix [93].…”

Section: Discussionmentioning

confidence: 99%

Synthesis and Evaluation of a Thermoresponsive Degradable Chitosan-Grafted PNIPAAm Hydrogel as a “Smart” Gene Delivery System

et al. 2020

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Thermoresponsive hydrogels demonstrate tremendous potential as sustained drug delivery systems. However, progress has been limited as formulation of a stable biodegradable thermosensitive hydrogel remains a significant challenge. In this study, free radical polymerization was exploited to formulate a biodegradable thermosensitive hydrogel characterized by sustained drug release. Highly deacetylated chitosan and N-isopropylacrylamide with distinctive physical properties were employed to achieve a stable, hydrogel network at body temperature. The percentage of chitosan was altered within the copolymer formulations and the subsequent physical properties were characterized using 1H-NMR, FTIR, and TGA. Viscoelastic, swelling, and degradation properties were also interrogated. The thermoresponsive hydrogels were loaded with RALA/pEGFP-N1 nanoparticles and release was examined. There was sustained release of nanoparticles over three weeks and, more importantly, the nucleic acid cargo remained functional and this was confirmed by successful transfection of the NCTC-929 fibroblast cell line. This tailored thermoresponsive hydrogel offers an option for sustained delivery of macromolecules over a prolonged considerable period.

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DNA vaccination via RALA nanoparticles in a microneedle delivery system induces a potent immune response against the endogenous prostate cancer stem cell antigen

Abstract: , H. O. (2019). DNA vaccination via RALA nanoparticles in a microneedle delivery system induces a potent immune response against the endogenous prostate cancer stem cell antigen. Acta Biomaterialia.

Cited by 83 publications

References 40 publications

Current trends in polymer microneedle for transdermal drug delivery

Current trends in polymer microneedle for transdermal drug delivery

Electrospun Biomaterials in the Treatment and Prevention of Scars in Skin Wound Healing

Synthesis and Evaluation of a Thermoresponsive Degradable Chitosan-Grafted PNIPAAm Hydrogel as a “Smart” Gene Delivery System

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