DNA Vaccination with the Major Outer‐Membrane Protein Gene Induces Acquired Immunity to<i>Chlamydia trachomatis</i>(Mouse Pneumonitis) Infection

Zhang, Dongji; Yang, Xi; Berry, Jody D.; Shen, Caixia; McClarty, Grant; Brunham, Robert C.

doi:10.1086/516545

Cited by 103 publications

(75 citation statements)

References 23 publications

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“…We have demonstrated in previous studies that DNA vaccination using the chlamydial MOMP gene (omp1) induces both IL-12 and IL-10 production but provides significant protection (50). Moreover, we have also shown that vaccination with live, compared with dead, chlamydial organisms induced both higher IL-12 as well as IL-10 but provided strong protection (41).…”

Section: Discussionmentioning

confidence: 96%

Adoptive Transfer of CD8α+ Dendritic Cells (DC) Isolated from Mice Infected with Chlamydia muridarum Are More Potent in Inducing Protective Immunity Than CD8α− DC

Bilenki

Wang

Yang

et al. 2006

The Journal of Immunology

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Chlamydial infections are serious public health concerns worldwide. In this study, we examined the role of dendritic cell (DC) subsets in inducing protective immunity against chlamydial infection using an adoptive transfer approach. We found that CD11c+CD8α+ (double-positive, DP) DC, compared with CD11c+CD8α− (single-positive, SP) DC isolated from infected mice, are more potent inducers of protective immunity. Specifically, mice pretreated with DPDC from infected mice, upon infection with Chlamydia trachomatis mouse pneumonitis (MoPn), experienced significantly less severe body weight loss and in vivo chlamydial growth. Analysis of MoPn-driven cytokine production by immune cells revealed that mice that were treated with DPDC produced significantly higher levels of Th1 (TNF-α, IFN-γ, and IL-12) but lower levels of Th2 (IL-4, IL-5, and IL-13)-related cytokines than the recipients of SPDC following infection challenge. Moreover, DPDC-treated mice displayed significantly higher levels of MoPn-specific IgG2a production and delayed-type hypersensitivity responses compared with SPDC-treated mice. Furthermore, DPDC isolated from infected mice produced higher amounts of IL-12 and IL-10 in vitro in comparison with SPDC. These data indicate that CD8α+ DC have a significantly higher capacity in inducing protective immunity compared with CD8α− DC, demonstrating the crucial role of DC1-like cells in eliciting protection against C. trachomatis infection

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Section: Discussionmentioning

confidence: 96%

Adoptive Transfer of CD8α+ Dendritic Cells (DC) Isolated from Mice Infected with Chlamydia muridarum Are More Potent in Inducing Protective Immunity Than CD8α− DC

Bilenki

Wang

Yang

et al. 2006

The Journal of Immunology

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“…Recombinant MOMP, MOMP synthetic peptides, DNA vaccines encoding MOMP, and the passive transfer of MOMP-specific monoclonal antibodies have been evaluated for protective efficacy. All studies have yielded disappointing results since protective immunity either was not generated or was partial, at best (18,25,32,36,67,70,71,79,102,120,121). The reason for the ineffectiveness of MOMP as a vaccine is not known, but it may result from adjuvants or delivery systems that ineffectively target genital tract mucosae or from use of MOMP immunogens that do not mimic the native structure of the protein (69).…”

Section: Vaccine Prospectives and Challengesmentioning

confidence: 99%

Immunity to Murine Chlamydial Genital Infection

2002

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“…DNA vaccination has already been used to elicit protective immune responses against Chlamydia trachomatis, Chlamydophila pneumoniae and Chlamydophila psittaci [10,15,16]. In preliminary studies, a DNA vaccine encoding the heat shock protein (hsp) DnaK (also called Hsp70) of Chlamydophila abortus induced a humoral response with an IgG2a predominant isotype [8].…”

Section: Introductionmentioning

confidence: 99%

Proteic boost enhances humoral response induced by DNA vaccination with the dnaK gene of Chlamydophila abortus but fails to protect pregnant mice against a virulence challenge

Héchard¹,

Grépinet²,

Rodolakis³

2003

Vet. Res.

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-In order to enhance the quantity and the protective properties of the antibodies induced by DNA vaccination with the heat shock protein dnaK gene of Chlamydophila abortus AB7 as well as to elicit an efficient cellular immune response, we vaccinated mice with a DNA prime followed by a boost with the recombinant DnaK protein. In non-pregnant mice, this strategy induced the same predominance of the IgG2a isotype as DNA immunization alone with a substantial increased antibody level. The induced antibodies had no in vitro neutralizing properties on C. abortus infectivity. Moreover, the proteic boost probably failed to elicit an efficient cellular immune response since the pregnant or non-pregnant mice were not protected against the bacterial challenge.DNA immunization / proteic boost / Chlamydophila abortus / DnaK / Hsp70

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DNA Vaccination with the Major Outer‐Membrane Protein Gene Induces Acquired Immunity toChlamydia trachomatis(Mouse Pneumonitis) Infection

Cited by 103 publications

References 23 publications

Adoptive Transfer of CD8α+ Dendritic Cells (DC) Isolated from Mice Infected with Chlamydia muridarum Are More Potent in Inducing Protective Immunity Than CD8α− DC

Adoptive Transfer of CD8α+ Dendritic Cells (DC) Isolated from Mice Infected with Chlamydia muridarum Are More Potent in Inducing Protective Immunity Than CD8α− DC

Immunity to Murine Chlamydial Genital Infection

Proteic boost enhances humoral response induced by DNA vaccination with the dnaK gene of Chlamydophila abortus but fails to protect pregnant mice against a virulence challenge

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