DNA Vaccines against Infectious Diseases and Cancer

Han, Deokjae; Weiner, David B.; Sin, Jeong-Im

doi:10.4062/biomolther.2010.18.1.001

Cited by 4 publications

(4 citation statements)

References 151 publications

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“…Electroporation (EP), the administration of electrical pulses to muscle following DNA injection, has been shown to enhance the immunogenicity of DNA vaccines in a wide variety of small and large animal models (Han et al, 2010). The potential of EP delivery for clinical applications has been also suggested (Luxembourg et al, 2007;Bodles-Brakhop et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Antitumor Therapeutic and Antimetastatic Activity of Electroporation-Delivered Human Papillomavirus 16 E7 DNA Vaccines: A Possible Mechanism for Enhanced Tumor Control

Lee

Park

Cheong

et al. 2011

DNA and Cell Biology

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DNA vaccines are known to be lacking in immunogenicity in humans. Presently, electroporation (EP) is thought to overcome this limitation. Here, we investigate whether human papillomavirus 16 E7 DNA vaccines delivered by EP might elicit potent antitumor activity in animal cervical cancer models, with a focus on the underlying mechanism(s). Intramuscular (IM)-EP delivery of E7 DNA vaccines induced more potent antitumor therapeutic and antimetastatic activity compared with IM delivery. Moreover, the tumor-controlled animals by IM-EP possessed long-term memory responses to parental tumor cells. This improved antitumor effect was concomitant with augmented Ag-specific CTL activities. IM-EP also induced IgG and Th-cell responses higher than IM delivery. Finally, IM-EP resulted in more antigen production in and more attraction of immune cells into the site of DNA injection, suggesting that these biological and immunological changes made by IM-EP might be responsible for enhanced CTL activities and antitumor resistance. Thus, this study shows that IM-EP can induce more potent antitumor activity by augmenting CTL responses possibly through more antigen production in and more attraction of immune cells into the muscle sites. This study also suggests that IM-EP of E7 DNA vaccines might be a potential approach toward treating patients with cervical cancer.

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Section: Introductionmentioning

confidence: 99%

Antitumor Therapeutic and Antimetastatic Activity of Electroporation-Delivered Human Papillomavirus 16 E7 DNA Vaccines: A Possible Mechanism for Enhanced Tumor Control

Lee

Park

Cheong

et al. 2011

DNA and Cell Biology

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“…44 23 ). These preclinical studies demonstrated that antigen codon optimization, CD4+ T helper cell epitope domain conjugation, and antigen targeting to specific intracellular compartments might be promising strategies to augment cancer Ag-specific CTL responses and antitumor activity.…”

Section: Dna Manipulation and The Use Of Xenogeneic Antigensmentioning

confidence: 96%

“…Previously, our group demonstrated using a herpes simplex virus (HSV)-2 gD DNA vaccine model that the naked injection of gD DNA vaccines along with plasmid DNA encoding Th0-, Th1-, and Th2-type cytokines, chemokines and co-stimulatory molecules can modulate Ag-specific antibody and cellular responses, and enhance protective immunity against HSV-2 challenges (reviewed in 23 ). These data suggest that these immune stimulatory molecules could be useful as cancer vaccine adjuvants for enhancing Ag-specific CTL responses, which are required for cancer eradication.…”

Section: Co-injection Of Cytokines and Other Moleculesmentioning

confidence: 99%

DNA vaccines, electroporation and their applications in cancer treatment

Lee

Danishmalik

Sin

2015

Human Vaccines & Immunotherapeutics

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Numerous animal studies and recent clinical studies have shown that electroporation-delivered DNA vaccines can elicit robust Ag-specific CTL responses and reduce disease severity. However, cancer antigens are generally poorly immunogenic, requiring special conditions for immune response induction. To date, many different approaches have been used to elicit Ag-specific CTL and anti-neoplastic responses to DNA vaccines against cancer. In vivo electroporation is one example, whereas others include DNA manipulation, xenogeneic antigen use, immune stimulatory molecule and immune response regulator application, DNA prime-boost immunization strategy use and different DNA delivery methods. These strategies likely increase the immunogenicity of cancer DNA vaccines, thereby contributing to cancer eradication. However, cancer cells are heterogeneous and might become CTL-resistant. Thus, understanding the CTL resistance mechanism(s) employed by cancer cells is critical to develop counter-measures for this immune escape. In this review, the use of electroporation as a DNA delivery method, the strategies used to enhance the immune responses, the cancer antigens that have been tested, and the escape mechanism(s) used by tumor cells are discussed, with a focus on the progress of clinical trials using cancer DNA vaccines.

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“…DNA vaccines have become a promising alternative for the treatment and prevention of different infectious diseases [1][2][3], such as tuberculosis [4]. Typically, this kind of vaccine consists of a modified bacterial plasmid DNA coupled to a gene sequence that encodes a desired protein.…”

Section: Introductionmentioning

confidence: 99%

Effectiveness, against tuberculosis, of pseudo-ternary complexes: Peptide-DNA-cationic liposome

Rosada

Silva

Santana

et al. 2012

Journal of Colloid and Interface Science

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We report the effects of a synthetic peptide designed to act as a nuclear localization signal on the treatment of tuberculosis. The peptide contains 21 amino acid residues with the following specific domains: nuclear localization signal from SV 40T, cationic shuttle sequence, and cysteamide group at the C-terminus. The peptide was complexed with the plasmid DNAhsp65 and incorporated into cationic liposomes, forming a pseudo-ternary complex. The same cationic liposomes, composed of egg chicken L-α-phosphatidylcholine, 1,2-dioleoyl-3-trimethylammonium-propane, and 1,2-dioleoyl-3-trimethylammonium-propane (2:1:1M), were previously evaluated as a gene carrier for tuberculosis immunization protocols with DNAhsp65. The pseudo-ternary complex presented a controlled size (250 nm), spherical-like shape, and various lamellae in liposomes as evaluated by transmission electron microscopy. An assay of fluorescence probe accessibility confirmed insertion of the peptide/DNA into the liposome structure. Peptide addition conferred no cytotoxicity in vitro, and similar therapeutic effects against tuberculosis were seen with four times less DNA compared with naked DNA treatment. Taken together, the results indicate that the pseudo-ternary complex is a promising gene vaccine for tuberculosis treatment. This work contributes to the development of multifunctional nanostructures in the search for strategies for in vivo DNA delivery.

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DNA Vaccines against Infectious Diseases and Cancer

Cited by 4 publications

References 151 publications

Antitumor Therapeutic and Antimetastatic Activity of Electroporation-Delivered Human Papillomavirus 16 E7 DNA Vaccines: A Possible Mechanism for Enhanced Tumor Control

Antitumor Therapeutic and Antimetastatic Activity of Electroporation-Delivered Human Papillomavirus 16 E7 DNA Vaccines: A Possible Mechanism for Enhanced Tumor Control

DNA vaccines, electroporation and their applications in cancer treatment

Effectiveness, against tuberculosis, of pseudo-ternary complexes: Peptide-DNA-cationic liposome

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