Do adipose tissue-derived mesenchymal stem cells have the same osteogenic and chondrogenic potential as bone marrow-derived cells?

Abstract

The results of our study suggest that the ATMSCs may have an inferior potential for both osteogenesis and chondrogenesis compared with the BMMSCs, and these cast doubts on the value of adipose tissue as a source of MSCs.

Chondrogenic differentiation of adipose tissue‐derived mesenchymal stem cells: Greater doses of growth factor are necessary

“…[11][12][13][14] One study that compared chondrogenesis in ATMSCs and BMMSCs from the same individual reported that after 2 weeks of pellet culture, the amount of GAG and the expression of type II collagen mRNA was 120% and 500-to 5000-fold higher in BMMSCs than in ATMSCs. 11 Sakaguchi et al 12 showed that human bone marrow-or synoviumderived stem cells had greater chondrogenic and osteogenic potential than adipose tissue-derived stem cells.…”

“…We also demonstrated that human ATMSCs have much weaker presentation as chondrogenic cells in both cell morphology and matrix production when cultured under the same chondrogenic conditions as BMMSCs. 13 On the other hand, some studies that examined different growth factors suggested that effective chondrogenesis might be achieved with the addition of BMPs. 15,16 Although the causes of the different response to growth factors are largely unknown and warrant extensive investigation, the discrepancy in the number of receptors to these factors is suggested to be one of them.…”

Adipose-derived Mesenchymal Stem Cells Are Phenotypically Superior for Regeneration in the Setting of Osteonecrosis of the Femoral Head

“…Despite having a proliferation advantage, numerous studies have shown inferior osteogenesis from aMSCs compared with bmMSCs [14,18,20,27,31,35]. These studies from in vitro experiments and animal models have questioned the ability of aMSCs for regenerative orthopaedics.…”

State of the art and future perspectives of articular cartilage regeneration: a focus on adipose-derived stem cells and platelet-derived products

“…A considerable number of papers have been published since 2003, addressing the question of which cell type, ASC or BMSC, possesses a higher chondrogenic potential (Table 1). A variety of authors concluded that BMSC can be more easily differentiated towards the chondrogenic lineage than ASC (Afizah et al, 2007;Danisovic et al, 2009;Huang et al, 2005;Im et al, 2005;Jakobsen et al, 2009;Kisiday et al, 2008;Koga et al, 2008;Liu et al, 2007;Vidal et al, 2008). However, Diekman et al (2010) pointed out that ASC and BMSC require unequal growth factor treatment for chondrogenic induction.…”

“…Regarding aggrecan expression, BMSC respond better to TGFβ, while ASC require BMP-6 (Jakobsen et al, 2009) Human In hyaluronic acid scaffolds chondrogenesis of BMSC was higher compared to ASC (Liu et al, 2007) Human BMSC differentiate better into osteoblasts and chondrocytes, while ASC have higher adipogenic differentiation potential (Afizah et al, 2007) Human BMSC and ASC from the same donor: BMSC are more suitable for cartilage tissue engineering than ASC (Huang et al, 2005) Human A patient-matched study: under the described conditions, BMSC demonstrated a higher chondrogenic potential (Im et al, 2005) Human Results of this study suggest that ASC have inferior capacity for chondrogenic differentiation (Winter et al, 2003) Human There are no differences in the expression of chondrogenic marker genes in 2D cultures between ASC and BMSC. In 3D cultures BMSC expressed a gene profile similar to that of osteoarthritic cartilage (Koga et al, 2008) Rabbit BMSC have higher chondrogenic potential compared to ASC in vitro and in vivo Rat There are no major differences between ASC and BMSC regarding the chondrogenic differentiation potential (Kisiday et al, 2008) Equine Superior chondrogenesis of BMSC was demonstrated in comparison to ASC (Vidal et al, 2008) Equine Superior chondrogenesis of BMSC was demonstrated in comparison to ASC Table 2.…”