Do G-protein coupled estrogen receptor and bisphenol A analogs influence on Leydig cell epigenetic regulation in immature boar testis ex vivo?

Pawlicki, Piotr; Duliban, Michał; Tuz, R.; Ptak, Anna; Miłoń, Agnieszka; Górowska-Wójtowicz, Ewelina; Tworzydło, Wacław; Płachno, Bartosz J.; Bilińska, Barbara; Knapczyk-Stwora, Katarzyna; Kotula–Balak, Małgorzata

doi:10.1016/j.anireprosci.2019.06.006

Cited by 19 publications

(9 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An in vitro study on testicular fragments culture from 7-day-old male pigs exposed to BPA demonstrated a downregulation of EXPO5 and Dicer genes and an upregulation of Drosha and AGO2 genes, involved in miRNA pathways. Also, Leydig cells' morphology was not altered but interstitial tissue collagen was increased ( 25 ).…”

Section: Methodsmentioning

confidence: 99%

Bisphenol A-Induced Epigenetic Changes and Its Effects on the Male Reproductive System

et al. 2020

View full text Add to dashboard Cite

Bisphenol A (BPA) is a widespread chemical agent which can exert detrimental effects on the male reproductive system. Exposure to BPA has been shown to induce several epigenetic modifications in both animal and human cells. Specifically, BPA could not only modify the methylation pattern of multiple genes encoding proteins related to reproductive physiology but also directly influence the genes responsible for DNA methylation. BPA effects include hormonal alterations, microscopic and macroscopic alteration of male reproductive organs, and inheritable epigenetic changes involving human reproduction. BPA exposure was also linked to prostate cancer. This review aims to show the current scenario of BPA-induced epigenetic changes and its effects on the male reproductive system. Possible strategies to counter the toxic effect of BPA were also addressed.

show abstract

Section: Methodsmentioning

confidence: 99%

Bisphenol A-Induced Epigenetic Changes and Its Effects on the Male Reproductive System

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Dysregulation of testicular steroidogenic function by BPA through its action on miRNA has been demonstrated in Leydig cells isolated from murine testis [48]. Recently, Pawlicki and colleagues, using G15, xenoestrogen BPA and its derivatives TBBPA and TCBPA, clarified the GPER involvement on epigenetic regulation in immature boar Leydig cells [49]. The authors suggested that both G15 and different xenoestrogens, except for BPA and G15 plus TCBPA, reduced the GPER protein expression.…”

Section: Gper In Leydig Cellsmentioning

confidence: 99%

“…Testicular Physiology Testicular Tumors SPG GPER activation induces proliferation [84][85][86] GPER is overexpressed in seminoma and embryonal carcinoma [119], (whereas ERα is missing [120]) GPER mediates estrogen and xenoestrogen-dependent proliferation in seminomas [123][124][125]128,129] SPT GPER activation induces apoptosis [31,32,88] RS GPER activation induces apoptosis [30] LC GPER is involved in: LC morphology and function [38] LCT [where ERα is overexpressed]: GPER activation decrease cell proliferation and induces apoptosis [130] Xenoestrogens decrease GPER expression favoring LCT progression [133] GPER is involved in LCT control of lipid metabolism and steroidogenesis (interaction with PPAR) [134] LC androgens production [40] LC lipophagy inhibition [43] LC miRNA biogenesis [49] LC development and function [PPAR crosstalk] [46] SC GPER activation induces proliferation in immature testis [65][66][67][68] Sertoli cell tumors: GPER is expressed but the role has been not identified. In interstitial compartment, GPER appears to play an important role in regulating estrogen-dependent lipid homeostasis and testosterone biosynthesis that occur in Leydig cells.…”

Section: Cell Typesmentioning

confidence: 99%

Role of GPER-Mediated Signaling in Testicular Functions and Tumorigenesis

Chimento

Luca

Nocito

et al. 2020

Cells

View full text Add to dashboard Cite

Estrogen signaling plays important roles in testicular functions and tumorigenesis. Fifteen years ago, it was discovered that a member of the G protein-coupled receptor family, GPR30, which binds also with high affinity to estradiol and is responsible, in part, for the rapid non-genomic actions of estrogens. GPR30, renamed as GPER, was detected in several tissues including germ cells (spermatogonia, spermatocytes, spermatids) and somatic cells (Sertoli and Leydig cells). In our previous review published in 2014, we summarized studies that evidenced a role of GPER signaling in mediating estrogen action during spermatogenesis and testis development. In addition, we evidenced that GPER seems to be involved in modulating estrogen-dependent testicular cancer cell growth; however, the effects on cell survival and proliferation depend on specific cell type. In this review, we update the knowledge obtained in the last years on GPER roles in regulating physiological functions of testicular cells and its involvement in neoplastic transformation of both germ and somatic cells. In particular, we will focus our attention on crosstalk among GPER signaling, classical estrogen receptors and other nuclear receptors involved in testis physiology regulation.

show abstract

“…Several recent studies revealed GPER presence and its implication in the testes functions of various animals [25,26,27,28]. Moreover, the association of GPER and estrogen-related receptors or PPAR interaction in the control of healthy and tumor testicular cells in rodents and humans was previously demonstrated by us [29,30,6,31]. Nowadays, the GPER antagonist is monitored as a potential therapeutic drug for male infertility treatment [32].…”

Section: Introductionmentioning

confidence: 95%

Peroxisome Proliferator-Activated Receptor γ, but Not α or G-Protein Coupled Estrogen Receptor Drives Functioning of Postnatal Boar Testis—Next Generation Sequencing Analysis

Duliban

Pawlicki

Gurgul

et al. 2021

Animals

View full text Add to dashboard Cite

Porcine tissue gene expression is highly similar to the expression of homologous genes in humans. Based on this fact, the studies on porcine tissues can be employed to understand human physiology and to predict or treat diseases. Our prior studies clearly showed that there was a regulatory partnership of the peroxisome proliferator-activated receptor (PPAR) and the G-protein coupled membrane estrogen receptor (GPER) that relied upon the tumorigenesis of human and mouse testicular interstitial cells, as well as the PPAR-estrogen related receptor and GPER–xenoestrogen relationships which affected the functional status of immature boar testes. The main objective of this study was to identify the biological processes and signaling pathways governed by PPARα, PPARγ and GPER in the immature testes of seven-day-old boars after pharmacological receptor ligand treatment. Boar testicular tissues were cultured in an organotypic system with the respective PPARα, PPARγ or GPER antagonists. To evaluate the effect of the individual receptor deprivation in testicular tissue on global gene expression, Next Generation Sequencing was performed. Bioinformatic analysis revealed 382 transcripts with altered expression. While tissues treated with PPARα or GPER antagonists showed little significance in the enrichment analysis, the antagonists challenged with the PPARγ antagonist displayed significant alterations in biological processes such as: drug metabolism, adhesion and tubule development. Diverse disruption in the Notch signaling pathway was also observed. The findings of our study proposed that neither PPARα nor GPER, but PPARγ alone seemed to be the main player in the regulation of boar testes functioning during early the postnatal developmental window.

show abstract

Do G-protein coupled estrogen receptor and bisphenol A analogs influence on Leydig cell epigenetic regulation in immature boar testis ex vivo?

Cited by 19 publications

References 63 publications

Bisphenol A-Induced Epigenetic Changes and Its Effects on the Male Reproductive System

Bisphenol A-Induced Epigenetic Changes and Its Effects on the Male Reproductive System

Role of GPER-Mediated Signaling in Testicular Functions and Tumorigenesis

Peroxisome Proliferator-Activated Receptor γ, but Not α or G-Protein Coupled Estrogen Receptor Drives Functioning of Postnatal Boar Testis—Next Generation Sequencing Analysis

Contact Info

Product

Resources

About