Do Local Anesthetics Have Antibacterial Effect on Staphylococcus aureus Under In Vivo Conditions? An Experimental Study

Köse, Aydan Ayşe; Karabaggli, Yakup; Kiremitci, Abdurrahman; Kocman, Emre A.; Çetin, Cengiz

doi:10.1111/j.1524-4725.2010.01559.x

Cited by 16 publications

(9 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Antimicrobial effects of LAs have been demonstrated by in vitro and experimental studies in the literature. In experimental studies; Lu et al, Stratford et al, and Kose et al reported that lidocaine showed antimicrobial effects on S aureus .…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial effects of local anaesthetics

Kesici

Demırci

Kesi̇ci̇

2019

International Wound Journal

View full text Add to dashboard Cite

After the introduction of cocaine to the medical practice, local anaesthetics (LA) became essential in pain control. LA infiltration along the incision may be used to provide surgical anaesthesia or postoperative analgesia. This study aimed to compare the antimicrobial effects of the topical antimicrobial agent mupirocine with those of the LA lidocaine and the combination of lidocaine and adrenalin. In our study, the in vitro antimicrobial effects of 1 mL sterile saline, 20 mg/mL mupirocine, 20 mg/mL Lidocaine, and 20 mg/mL Lidocaine and Adrenaline were tested against Staphylococcus aureus American type culture collection (ATCC) 29213, Pseudomonas aeruginosa ATCC 27853, and Escherichia coli ATCC 25922 as Group C (Control), Group M (Mupirocine), Group L (Lidocaine), and Group LA (Lidocaine + adrenaline), respectively. S aureus ATCC 29213, P aeruginosa ATCC 27853, and E coli ATCC 25922 were cultured onto Mueller–Hinton agar (Oxoid, UK) plates for 18 to 24 hours at 37°C. Colonies from these plates were suspended in sterile saline and a 0.5 McFarland turbidity standard suspension (corresponding to 1.5 × 108 CFU/mL) of each isolate was prepared. S Aureus ATCC 29213 inhibition zone diameter values of Group M, Group LA, and Group L were significantly higher compared with the group C (P ˂ 0.05). P aeruginosa ATCC 27853 inhibition zone diameter values of Group M and Group LA were significantly higher compared with the group C (P ˂ 0.05). E coli ATCC 25922 inhibition zone diameter values of Group M, Group LA, and Group L were significantly higher compared to the group C (P ˂ 0.05). LA infiltration along the incision may be used to provide surgical anaesthesia or postoperative analgesia. Considering that LAs show antimicrobial effects besides their analgesic effects, they may contribute to preventing the development and reducing the rate of surgical infections, decreasing the requirement to administer antibiotics. However, caution should be exercised not to antagonise the effective treatment of surgical infections, remembering that controversy on the antimicrobial effects of LAs remains in the literature. Therefore, further comprehensive studies with larger patient populations are warranted to demonstrate the antimicrobial effects of LAs.

show abstract

Section: Discussionmentioning

confidence: 99%

Antimicrobial effects of local anaesthetics

Kesici

Demırci

Kesi̇ci̇

2019

International Wound Journal

View full text Add to dashboard Cite

show abstract

“…There are in vivo studies that used lidocaine in S aureus -infected wounds and have shown decreased bacterial counts in animals treated with this anesthetic, [ 28 , 29 ] and other studies showed that this anesthetic did not have antimicrobial activity. [ 30 , 31 ] The mechanisms of action considered for lidocaine bactericidal activity are cell wall disruption, altered DNA synthesis and cellular respiration dysfunction. [ 29 ] In precontamination washing, pure lidocaine inhibited colonization by P mirabilis in silicone prostheses and decreased E faecalis colonization in ePTFE prostheses, while lidocaine solution decreased colonization by P mirabilis in silicone prostheses but had no effect in ePTFE prostheses.…”

Section: Discussionmentioning

confidence: 99%

Does washing medical devices before and after use decrease bacterial contamination?

Nai¹,

Medina²,

Martelli³

et al. 2021

Medicine

View full text Add to dashboard Cite

Surface treatment of medical devices may be a way of avoiding the need for replacement of these devices and the comorbidities associated with infection. The aim of this study was to evaluate whether pre- and postcontamination washing of 2 prostheses with different textures can decrease bacterial contamination. The following microorganisms were evaluated: Staphylococcus aureus , Staphylococcus epidermidis , Proteus mirabilis and Enterococcus faecalis . Silicone and expanded polytetrafluoroethylene vascular prostheses were used and divided into 3 groups: prostheses contaminated; prostheses contaminated and treated before contamination; and prostheses contaminated and treated after contamination. Treatments were performed with antibiotic solution, chlorhexidine and lidocaine. After one week of incubation, the prostheses were sown in culture medium, which was incubated for 48 hours. The area of colony formation was evaluated by fractal dimension, an image analysis tool. The antibiotic solution inhibited the growth of S epidermidis and chlorhexidine decrease in 53% the colonization density for S aureus in for both prostheses in the pre-washing. In postcontamination washing, the antibiotic solution inhibited the growth of all bacteria evaluated; there was a 60% decrease in the colonization density of S aureus and absence of colonization for E faecalis with chlorhexidine; and lidocaine inhibited the growth of S aureus in both prostheses. Antibiotic solution showed the highest efficiency in inhibiting bacterial growth, especially for S epidermidis , in both washings. Lidocaine was able to reduce colonization by S aureus in post-contamination washing, showing that it can be used as an alternative adjuvant treatment in these cases.

show abstract

“…Tamanai-Shacoori [11] and colleagues tested tramadol in cultures of Escherichia coli and Staphylococcus aureus at two different concentrations. Tramadol at a concentration of 12.5 mg/mL had an inhibitory effect by reducing the Escherichia coli and Staphylococcus aureus growth, respectively, whereas, at 25 mg/mL, the inhibitory effect was increased for Staphylococcus aureus, but tramadol induced a bactericidal effect for Escherichia coli [12]. In another study, more strains were used-Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, and Ps.…”

Section: Opioidsmentioning

confidence: 97%

Antimicrobial Properties on Non-Antibiotic Drugs in the Era of Increased Bacterial Resistance

et al. 2020

View full text Add to dashboard Cite

In recent years, due to the dramatic increase in and global spread of bacterial resistance to a number of commonly used antibacterial agents, many studies have been directed at investigating drugs whose primary therapeutic purpose is not antimicrobial action. In an era where it is becoming increasingly difficult to find new antimicrobial drugs, it is important to understand these antimicrobial effects and their potential clinical implications. Numerous studies report the antibacterial activity of non-steroidal anti-inflammatory drugs, local anaesthetics, phenothiazines such as chlorpromazine, levomepromazine, promethazine, trifluoperazine, methdilazine and thioridazine, antidepressants, antiplatelets and statins. Several studies have explored a possible protective effect of statins inreducing the morbidity and mortality of many infectious diseases. Various non-antibiotic agents exhibit antimicrobial activity via multiple and different mechanisms of action. Further studies are required in the field to further investigate these antimicrobial properties in different populations. This is of paramount importance in the antimicrobial resistance era, where clinicians have limited therapeutic options to combat problematic infections.

show abstract

Do Local Anesthetics Have Antibacterial Effect on Staphylococcus aureus Under In Vivo Conditions? An Experimental Study

Abstract: The results of the present study did not show any antimicrobial activity of above-mentioned local anesthetics in surgically created wounds of rats.

Cited by 16 publications

References 9 publications

Antimicrobial effects of local anaesthetics

Antimicrobial effects of local anaesthetics

Does washing medical devices before and after use decrease bacterial contamination?

Antimicrobial Properties on Non-Antibiotic Drugs in the Era of Increased Bacterial Resistance

Contact Info

Product

Resources

About