Do Preservation Solutions Protect Rat Cremaster Microcirculation during Ischemia and Reperfusion?

Bastiaanse, Jacqueline; Slaaf, Dick W.; Egbrink, Mirjam G.A. oude; Boeckx, W.; Kon, Moshe

doi:10.1016/j.jss.2004.11.035

Cited by 6 publications

(1 citation statement)

References 32 publications

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“…In contrast, Bastiaanse et al . did not find any effect on capillary perfusion after 4–6 hrs of ischaemia using pre‐ischaemic perfusion of skeletal muscles with HTK , stasis of blood flow after reperfusion occurred in both pre‐ischaemic perfused and not perfused groups . Treatment with HTK in all groups in the presented study (continuous perfusion, singular flush and perfusion with oxygenation), as with all the other treatments, lead to a significant lower expression of Caspase‐3 after 60 min.…”

Section: Discussionmentioning

confidence: 47%

Ischaemia‐related cell damage in extracorporeal preserved tissue – new findings with a novel perfusion model

Taeger

Müller‐Seubert

Horch

et al. 2014

J Cellular Molecular Medi

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Tissue undergoing free transfer in transplant or reconstructive surgery always is at high risk of ischaemia-related cell damage. This study aims at assessing different procedures using an extracorporeal perfusion and oxygenation system to investigate the expression of hypoxia inducible factor (HIF)-1-α as marker for hypoxia and of the pro-apoptotic protein Caspase-3 in skeletal muscle to elucidate potential improvements in tissue conservation. Twenty-four porcine rectus abdominis muscles were assigned to five different groups and examined after they had been extracorporeally preserved for 60 min. time. Group I was left untreated (control), group II was perfused with a cardioplegic solution, group III was flushed with 10 ml of a cardioplegic solution and then left untreated. Group IV and V were perfused and oxygenated with either an isotone crystalloid solution or a cardioplegic solution. Among others, immunohistochemistry (Caspase-3 and HIF-1-α) of muscle samples was performed. Furthermore, oxygen partial pressure in the perfusate at the arterial and venous branch was measured. Expression of Caspase-3 after 60 min. was reduced in all groups compared to the control group. Furthermore, all groups (except group III) expressed less HIF-1-α than the control group. Oxygenation leads to higher oxygen levels at the venous branch compared to groups without oxygenation. Using an extracorporeal perfusion and oxygenation system cell damage could be reduced as indicated by stabilized expressions of Caspase-3 and HIF-1-α for 60 min. of tissue preservation. Complete depletion of oxygen at the venous branch can be prevented by oxygenation of the perfusate with ambient air.

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Section: Discussionmentioning

confidence: 47%