Do the intestinal microbiotas differ between paddlefish (<i>Polyodon spathala</i>
) and bighead carp (<i>Aristichthys nobilis</i>
) reared in the same pond?

Li, X.M.; Zhu, Yugui; Yan, Qingyun; Ringø, Einar; Yang, Deguo

doi:10.1111/jam.12626

Cited by 84 publications

(54 citation statements)

References 39 publications

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“…Although most of our isolates appear closely related, the fact that every fish and habitat we examined contained different isolates suggests that there are many diverse and unique strains of Shewanella that are adapted to specific ecological niches. In support of this hypothesis, it was recently reported that the intestinal microbiota of fish can be very specific to the fish species they colonize (70).…”

Section: Discussionmentioning

confidence: 77%

The Microbiota of Freshwater Fish and Freshwater Niches Contain Omega-3 Fatty Acid-Producing Shewanella Species

Dailey

McGraw

Jensen

et al. 2016

Appl Environ Microbiol

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Approximately 30 years ago, it was discovered that free-living bacteria isolated from cold ocean depths could produce polyunsaturated fatty acids (PUFA) such as eicosapentaenoic acid (EPA) (20:5n-3) or docosahexaenoic acid (DHA) (22:6n-3), two PUFA essential for human health. Numerous laboratories have also discovered that EPA-and/or DHA-producing bacteria, many of them members of the Shewanella genus, could be isolated from the intestinal tracts of omega-3 fatty acid-rich marine fish. If bacteria contribute omega-3 fatty acids to the host fish in general or if they assist some bacterial species in adaptation to cold, then cold freshwater fish or habitats should also harbor these producers. Thus, we undertook a study to see if these niches also contained omega-3 fatty acid producers. We were successful in isolating and characterizing unique EPA-producing strains of Shewanella from three strictly freshwater native fish species, i.e., lake whitefish (Coregonus clupeaformis), lean lake trout (Salvelinus namaycush), and walleye (Sander vitreus), and from two other freshwater nonnative fish, i.e., coho salmon (Oncorhynchus kisutch) and seeforellen brown trout (Salmo trutta). We were also able to isolate four unique free-living strains of EPA-producing Shewanella from freshwater habitats. Phylogenetic and phenotypic analyses suggest that one producer is clearly a member of the Shewanella morhuae species and another is sister to members of the marine PUFA-producing Shewanella baltica species. However, the remaining isolates have more ambiguous relationships, sharing a common ancestor with non-PUFA-producing Shewanella putrefaciens isolates rather than marine S. baltica isolates despite having a phenotype more consistent with S. baltica strains.

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Section: Discussionmentioning

confidence: 77%

The Microbiota of Freshwater Fish and Freshwater Niches Contain Omega-3 Fatty Acid-Producing Shewanella Species

Dailey

McGraw

Jensen

et al. 2016

Appl Environ Microbiol

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“…It is of interest to note that the most dominant genus in male and female largemouth bronze gudgeon was unclassified bacteria (phylum Proteobacteria and family Enterobacteriaceae) and Mycoplasma (phylum Tenericutes), respectively, which is different to the findings reported for other freshwater fish species [28, 38]. Ringø et al [9] showed that the abundance of Enterobacteriaceae were affected by protein sources.…”

Section: Discussionmentioning

confidence: 88%

“…The exterior surfaces were swabbed with 75 % ethanol before dissection of the whole intestine using sterile instruments (scissors and tweezers). The intestine of each individual fish was dissected out, and similar weight (about 0.2 g) of foregut, midgut and hindgut from each individual was collected and pooled together into a sterile tube as a single sample as described previously [38]. The individual intestinal contents were homogenized by vortexing briefly.…”

Section: Methodsmentioning

confidence: 99%

The influence of weight and gender on intestinal bacterial community of wild largemouth bronze gudgeon (Coreius guichenoti, 1874)

Yan

Ringø

et al. 2016

BMC Microbiol

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BackgroundLargemouth bronze gudgeon (Coreius guichenoti) is of economic importance in China, distributed in upstream regions of the Yangtze River in China. But it has recently dramatically declined and is close to elimination. However, there is little knowing about the character of its intestinal microbiota. This study was conducted to elucidate the intestinal microbiota of wild largemouth bronze gudgeon with different body weight and gender.ResultsThirty wild largemouth bronze gudgeon were measured for body length and body weight, and identified for male and female according to gonadal development, and thereafter the intestinal microbiota’s were assessed by MiSeq sequencing of 16S rRNA genes. The results revealed that phyla Proteobacteria and Tenericutes were dominant in wild largemouth bronze gudgeon intestine independent of the body weight. Shannon’s and Inverse Simpson’s diversity indexes were significant (P < 0.05) different between male and female fish. The phylum profile in the intestine of male fish revealed that phylum Proteobacteria was dominant, in contrast to female fish where five phyla Tenericutes, Proteobacteria, Firmicutes, Fusobacteria and Spirochaetes were dominant. The genus profile revealed that genera Shewanella and Unclassified bacteria were dominant in male fish, while genus Mycoplasma was dominant in female fish.ConclusionsOur results revealed that the intestinal microbial community of wild largemouth bronze gudgeon was dominated by the phyla Proteobacteria and Tenericutes regardless of the different body weight, but the communities are significant different between male and female fish. These results provide a theoretical basis to understand the biological mechanisms relevant to the protection of the endangered fish species.

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“…Proteobacteria was the most prevalent phylum in the examined fish. This commonly‐found phylum was also observed in the intestines of other freshwater fish, such as: bighead carp Hypophthalmichthys nobilis (Richardson 1845) (Li et al ., ) and rainbow trout Oncorhynchus mykiss (Walbaum 1792) (Navarrete et al ., ). An analogous situation was observed in Midas cichlid Amphilophus citrinellus (Günther 1864) freshwater fish (Franchini et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Characterization of the gut microbiota in early life stages of pikeperch Sander lucioperca

Dulski

Zakęś

Ciesielski

2017

Journal of Fish Biology

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This study characterized the gastrointestinal microbiome of nine juvenile farmed pikeperch Sander lucioperca using a metagenomics approach based on bacterial 16S rRNA gene sequencing. Potential changes in the gut microbiota during 2 months of S. lucioperca juvenile life were investigated. Results revealed that gut microbiota was dominated by Proteobacteria (95-92%), while other phyla Firmicutes (1-1·5%) and Actinobacteria (0·9-1·5%) were less abundant. At the family level, fish-gut microbiota were dominated by Enterobacteriaceae, which constituted c. 83% of all DNA sequence reads. Such a situation was present in all of the examined fish except one, which showed a different proportion of particular microbial taxa than the other fish. In this fish, a higher relative abundance (%) of Fusobacteria (21·0%), Bacteroidetes (9·5%) and Firmicutes (7·5%) was observed. There were no significant differences in the gut microbiome structure at different stages of development in the examined fish. This may indicate that Proteobacteria inhabiting the gut microbiota at an early stage of life are a necessary component of the pikeperch microbiome that may support proper nutrition of the fish. The information obtained on the gut microbiome could be useful in determining juvenile S. lucioperca health and improving rearing conditions by welfare monitoring in aquaculture.

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Do the intestinal microbiotas differ between paddlefish (Polyodon spathala ) and bighead carp (Aristichthys nobilis ) reared in the same pond?

Cited by 84 publications

References 39 publications

The Microbiota of Freshwater Fish and Freshwater Niches Contain Omega-3 Fatty Acid-Producing Shewanella Species

The Microbiota of Freshwater Fish and Freshwater Niches Contain Omega-3 Fatty Acid-Producing Shewanella Species

The influence of weight and gender on intestinal bacterial community of wild largemouth bronze gudgeon (Coreius guichenoti, 1874)

Characterization of the gut microbiota in early life stages of pikeperch Sander lucioperca

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