The influence of weight and gender on intestinal bacterial community of wild largemouth bronze gudgeon (Coreius guichenoti, 1874)

Abstract

BackgroundLargemouth bronze gudgeon (Coreius guichenoti) is of economic importance in China, distributed in upstream regions of the Yangtze River in China. But it has recently dramatically declined and is close to elimination. However, there is little knowing about the character of its intestinal microbiota. This study was conducted to elucidate the intestinal microbiota of wild largemouth bronze gudgeon with different body weight and gender.ResultsThirty wild largemouth bronze gudgeon were measured for body length and body weight, and identified for male and female according to gonadal development, and thereafter the intestinal microbiota’s were assessed by MiSeq sequencing of 16S rRNA genes. The results revealed that phyla Proteobacteria and Tenericutes were dominant in wild largemouth bronze gudgeon intestine independent of the body weight. Shannon’s and Inverse Simpson’s diversity indexes were significant (P < 0.05) different between male and female fish. The phylum profile in the intestine of male fish revealed that phylum Proteobacteria was dominant, in contrast to female fish where five phyla Tenericutes, Proteobacteria, Firmicutes, Fusobacteria and Spirochaetes were dominant. The genus profile revealed that genera Shewanella and Unclassified bacteria were dominant in male fish, while genus Mycoplasma was dominant in female fish.ConclusionsOur results revealed that the intestinal microbial community of wild largemouth bronze gudgeon was dominated by the phyla Proteobacteria and Tenericutes regardless of the different body weight, but the communities are significant different between male and female fish. These results provide a theoretical basis to understand the biological mechanisms relevant to the protection of the endangered fish species.

Change in the intestinal bacterial community structure associated with environmental microorganisms during the growth of Eriocheir sinensis

“…The physiological state can include the health status of the aquatic organisms, their feeding status, and age. Studies on the health status of aquatic organisms have commonly detected differences in sex and body weight (Li, Yan, Einar, & Wu, ) and in feeds (Akter, Sutriana, Talpur, & Hashim, ; Giannenas, Karamaligas, Margaroni, & Pappas, ; Hao, Wu, Jakovlić, & Zou, ) and feeding methods (Rungrassamee, Klanchui, Maibunkaew, & Chaiyapechara, ; Tzeng, Pao, Chen, & Weng, ; Wang, Zhang, Li, & Lin, ). The differences in the gut microbiota at different ages and the exploration of the core bacteria have been studied frequently for human and model organism intestinal microbes; however, relatively little is known regarding gut bacteria in aquatic organisms.…”

Progress in fish gastrointestinal microbiota research

“…In addition, a recent study revealed that the intestinal microbial communities of wild largemouth bronze gudgeon ( Coreius guichenoti ) were significant different between male and female fish (Li et al . ). Stephens et al .…”

Dietary effects of soybean products on gut microbiota and immunity of aquatic animals: A review

“…However, LAB was not identified by 16S rRNA gene sequencing. Phenotypic males were used in this study, and this open for the speculation that differences in LAB composition may occur between gender, as some studies on aquatic animals have revealed differences in gut microbiota between male and female (Iehata, Valenzuela, & Riquelme, ; Li et al., ).…”

“…Another topic deserving attention is microbial degradation of antinutritional factors and genetically modified plants by the fish intestinal microbiota as no information is available (Ringø et al., ). When discussing microbial degradation of ANFs, it is worth to mention that the gut microbiota differs between genders in endothermic animals (Bolnick et al., ; Deusch et al., ; Mueller et al., ; Shastri, McCarville, Kalmokoff, Brooks, & Green‐Johnson, ; Zwielehner et al., ) as well as aquatic animals (Iehata et al., ; Li et al., ). Based on this fact, a controversial hypothesis can be put forward.…”