Does bisphenol A induce superfeminization in Marisa cornuarietis? Part II: Toxicity test results and requirements for statistical power analyses

Forbes, Valery E.; Aufderheide, John; Warbritton, Ryan; Hoeven, Nelly van der; Caspers, Norbert

doi:10.1016/j.ecoenv.2006.09.001

Cited by 34 publications

(31 citation statements)

References 12 publications

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“…In a study by Oehlmann et al [7] bisphenol A (BPA) exposure of the gastropod Marisa cornuarietis resulted in a 67 times lower EC 10 value at 20 • C than at 27 • C. Furthermore, the authors detected a masking of BPA effects during the snails main spawning season which may consequently lead to an underestimation of the substance s toxicity. A later study by Forbes et al [8] detected no effects on reproduction at a test temperature of 25 • C. Density might also effect reproduction in molluscs: Hatching of the Asian trampsnail Bradybaena similaris starts at day 21 when adults are kept at a low density and up to 15 days later at a higher adult density. [9] Consequently, insufficient standardization may lead to variations of the effect response and reduce reproducibility of results.…”

Section: Introductionmentioning

confidence: 95%

Effects of test media on reproduction inPotamopyrgus antipodarumand of pre-exposure population densities on sensitivity to cadmium in a reproduction test

Sieratowicz

Schulte‐Oehlmann

Wigh

et al. 2013

Journal of Environmental Science and Health, Part A

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Molluscan species can be affected by various anthropogenic substances. Yet, these effects are disregarded in chemical risk assessment as molluscs are unrepresented in standard OECD guidelines. The project "validation of a mollusc reproduction test" (Federal Environment Agency, code 371165417) deals with the development of a test method with the mudsnail Potamopyrgus antipodarum for OECD purposes. In this context, the influence on reproduction of both, different media and varying snail density, has been observed in independent experiments. Further, the impact of density on the outcome of subsequent cadmium (Cd) toxicity in a test has been investigated to refine the existing methodology. First, adult snails were kept in different test media for 12 weeks. Second, snail density was increased for 4 weeks to induce stress. Snails from each density scenario were used for another 4 weeks in a reproduction test at an equal density with 12 μg Cd/L, respectively. Significant differences in reproduction between medium groups were noted after 4 and 8, but not 12, weeks. Further, reproduction was significantly altered by snail density in the beakers but after subsequent 4 weeks at a constant density, no differences were observed between control groups. Cd reduced reproduction and this effect increased with snail density in the pre-exposure period, demonstrating that a previous stress factor may result in increased sensitivity to chemicals and underlines the need for more standardized breeding conditions to minimize effect variations. Based on the outcome of this study, an acclimatization period of 12 weeks must be guaranteed for specimens transferred to another medium. Further, 4 weeks of acclimatization are necessary after density stress. An additional 12 weeks density experiment showed that medium volume in each replicate can be decreased by half to save on chemicals, water and space during tests.

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Section: Introductionmentioning

confidence: 95%

Effects of test media on reproduction inPotamopyrgus antipodarumand of pre-exposure population densities on sensitivity to cadmium in a reproduction test

Sieratowicz

Schulte‐Oehlmann

Wigh

et al. 2013

Journal of Environmental Science and Health, Part A

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“…Forbes et al (2007), supported by the Bisphenol A Global Industry Group, evaluated the effects of bisphenol A on reproduction in the freshwater ramshorn snail (Marisa cornuarietis). Bisphenol A [purity not indicated] concentrations in test water were 0, 0.10, 1.0, 16, 160, and 640 mg/L.…”

Section: Utility (Adequacy) For Cerhr Evaluation Processmentioning

confidence: 99%

NTP‐CERHR expert panel report on the reproductive and developmental toxicity of bisphenol A

Chapin

Adams

Boekelheide

et al. 2008

Birth Defects Research Pt B

404

304

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“…reduction in male penis length). In direct conflict with these reports are those in which adult M. cornuarietis were exposed to BPA using a different experimental design to those employed in (Oehlmann et al, 2000) and (Oehlmann et al, 2006) showing clearly that these effects were not observed (Forbes et al, 2007). These conflicting reports have fuelled controversy (Dietrich et al, 2006) surrounding the true sensitivity of this species, and molluscs in general, to estrogen mimics and the perceived safety of the aquatic environment from the impacts of these xenoestrogens.…”

Section: Introductionmentioning

confidence: 84%

No substantial changes in estrogen receptor and estrogen-related receptor orthologue gene transcription in Marisa cornuarietis exposed to estrogenic chemicals

et al. 2013

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HighlightsER and ERR transcription levels were unaffected in Marisa cornuarietis exposed to 17β-estradiol or 4-tert-Octylphenol.The mollusc ER protein interacts with the phytoestrogen genistein in transfected HEK-293 cells.The mollusc ERR protein interacts weakly with bisphenol-A in transfected HEK-293 cells.The mollusc ER protein binds to the vertebrate consensus estrogen response element (ERE) sequence.

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Does bisphenol A induce superfeminization in Marisa cornuarietis? Part II: Toxicity test results and requirements for statistical power analyses

Cited by 34 publications

References 12 publications

Effects of test media on reproduction inPotamopyrgus antipodarumand of pre-exposure population densities on sensitivity to cadmium in a reproduction test

Effects of test media on reproduction inPotamopyrgus antipodarumand of pre-exposure population densities on sensitivity to cadmium in a reproduction test

NTP‐CERHR expert panel report on the reproductive and developmental toxicity of bisphenol A

No substantial changes in estrogen receptor and estrogen-related receptor orthologue gene transcription in Marisa cornuarietis exposed to estrogenic chemicals

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