No substantial changes in estrogen receptor and estrogen-related receptor orthologue gene transcription in Marisa cornuarietis exposed to estrogenic chemicals

Bannister, Richard; Beresford, Nicola; Granger, David; Pounds, Nadine; Rand‐Weaver, Mariann; White, Roger; Jobling, Susan; Routledge, Edwin J.

doi:10.1016/j.aquatox.2013.05.002

Cited by 35 publications

(16 citation statements)

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“…The phylogenetic position of the mollusc ER homologue with the vertebrate ER is well supported with a BMCMC posterior probability of 98% and bootstrap values of 0.88 and 0.79 ( Fig 1 ), supporting the suggestion that vertebrate and invertebrate ER diverged from a common ancestor, before the evolution of the deuterostomes [ 38 , 75 , 80 ]. Structural similarities between ER DBDs of molluscs, annelids, cephalochordates and vertebrates have been documented showing them to bind to and regulate transcription through estrogen response elements [ 36 , 40 , 74 , 81 , 82 ]. The ER LBD in invertebrates may have unique functions, since the mollusc ER homologue, at least in vitro (usually in mammalian reporter cell lines), appears to activate transcription constitutively in the absence of a ligand [ 36 , 37 , 39 , 40 ] although in annelids the ER has been shown to activate transcription in the presence of estrogens [ 82 ].…”

Section: Resultsmentioning

confidence: 99%

“…Structural similarities between ER DBDs of molluscs, annelids, cephalochordates and vertebrates have been documented showing them to bind to and regulate transcription through estrogen response elements [ 36 , 40 , 74 , 81 , 82 ]. The ER LBD in invertebrates may have unique functions, since the mollusc ER homologue, at least in vitro (usually in mammalian reporter cell lines), appears to activate transcription constitutively in the absence of a ligand [ 36 , 37 , 39 , 40 ] although in annelids the ER has been shown to activate transcription in the presence of estrogens [ 82 ]. We also identified a single estrogen-related receptor (ERR) in both B .…”

Section: Resultsmentioning

confidence: 99%

“…Other environmental pollutants could act through the estrogen receptor (ER) or androgen receptor (AR) and there is also some evidence of a role for androgen and estrogen-like molecules in the reproductive cycle of molluscs [ 31 , 32 ]. ER orthologues have previously been reported in molluscs (eg [ 33 – 37 ]) although their function is currently unconfirmed [ 38 , 36 , 39 , 37 , 40 ], since, for example, the identified ER homologue from the oyster Crassostrea gigas is unresponsive to estrogen [ 37 ], The presence of an ER in molluscs and the potential to respond to estrogens is confounded by the absence of aromatase (CYP19) [ 41 ], the enzyme required for the conversion of testosterone to estradiol, but it is possible that another enzyme catalyses the aromatase reaction [ 38 ]. The presence of ARs in molluscs has been inferred [ 32 , 42 ], but this remains an area of controversy [ 43 ] since homologues have not been identified despite investigations specifically searching for the gene [ 44 ].…”

Section: Introductionmentioning

confidence: 99%

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The Nuclear Receptors of Biomphalaria glabrata and Lottia gigantea: Implications for Developing New Model Organisms

et al. 2015

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Nuclear receptors (NRs) are transcription regulators involved in an array of diverse physiological functions including key roles in endocrine and metabolic function. The aim of this study was to identify nuclear receptors in the fully sequenced genome of the gastropod snail, Biomphalaria glabrata, intermediate host for Schistosoma mansoni and compare these to known vertebrate NRs, with a view to assessing the snail's potential as a invertebrate model organism for endocrine function, both as a prospective new test organism and to elucidate the fundamental genetic and mechanistic causes of disease. For comparative purposes, the genome of a second gastropod, the owl limpet, Lottia gigantea was also investigated for nuclear receptors. Thirty-nine and thirty-three putative NRs were identified from the B. glabrata and L. gigantea genomes respectively, based on the presence of a conserved DNA-binding domain and/or ligand-binding domain. Nuclear receptor transcript expression was confirmed and sequences were subjected to a comparative phylogenetic analysis, which demonstrated that these molluscs have representatives of all the major NR subfamilies (1-6). Many of the identified NRs are conserved between vertebrates and invertebrates, however differences exist, most notably, the absence of receptors of Group 3C, which includes some of the vertebrate endocrine hormone targets. The mollusc genomes also contain NR homologues that are present in insects and nematodes but not in vertebrates, such as Group 1J (HR48/DAF12/HR96). The identification of many shared receptors between humans and molluscs indicates the potential for molluscs as model organisms; however the absence of several steroid hormone receptors indicates snail endocrine systems are fundamentally different.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Nuclear Receptors of Biomphalaria glabrata and Lottia gigantea: Implications for Developing New Model Organisms

et al. 2015

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“…Expression of the ER and ERR homologs (CgNR3A and CgNR3B respectively) was at similar levels. Previous research in gastropod species showed differences in expression for male and female reproductive tissues and no differences for other tissues [ 85 , 86 , 91 ]. Nevertheless, gene expression was measured in whole individuals (not separated by tissue) and sex-dependent expression could still be neutralized by pooling of tissues (e.g., high expression in gonads, but low expression in mantle and vice versa).…”

Section: Discussionmentioning

confidence: 99%

Dynamics of nuclear receptor gene expression during Pacific oyster development

et al. 2016

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BackgroundNuclear receptors are a highly conserved set of ligand binding transcription factors, with essential roles regulating aspects of vertebrate and invertebrate biology alike. Current understanding of nuclear receptor regulated gene expression in invertebrates remains sparse, limiting our ability to elucidate gene function and the conservation of developmental processes across phyla. Here, we studied nuclear receptor expression in the early life stages of the Pacific oyster, Crassostrea gigas, to identify at which specific key stages nuclear receptors are expressedResultsWe used quantitative RT-PCR to determine the expression profiles of 34 nuclear receptors, revealing three developmental key stages, during which nuclear receptor expression is dynamically regulated: embryogenesis, mid development from gastrulation to trochophore larva, and late larval development prior to metamorphosis. Clustering of nuclear receptor expression patterns demonstrated that transcriptional regulation was not directly related to gene phylogeny, suggesting closely related genes may have distinct functions. Expression of gene homologs of vertebrate retinoid receptors suggests participation in organogenesis and shell-formation, as they are highly expressed at the gastrulation and trochophore larval initial shell formation stages. The ecdysone receptor homolog showed high expression just before larval settlement, suggesting a potential role in metamorphosis.ConclusionThroughout early oyster development nuclear receptors exhibited highly dynamic expression profiles, which were not confined by gene phylogeny. These results provide fundamental information on the presence of nuclear receptors during key developmental stages, which aids elucidation of their function in the developmental process. This understanding is essential as ligand sensing nuclear receptors can be disrupted by xenobiotics, a mode of action through which anthropogenic environmental pollutants have been found to mediate effects.Electronic supplementary materialThe online version of this article (doi:10.1186/s12861-016-0129-6) contains supplementary material, which is available to authorized users.

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“…In addition, Zhang et al (2014) demonstrated that BPA down-regulated 17 b-hydroxysteroid dehydrogenases (17b-HSDs), which control metabolism of steroids and fatty acids in Mytilus galloprovincialis digestive gland. BPA also increased the expression of estrogen receptor (ER) and estrogen-related receptor (ERR) genes in the reproductive tissue of the gastropod snail, Marisa cornuarietis at high concentrations (>10 −5 M, Bannister et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Acute and chronic effects of Bisphenol A on hormonal disruption and histological alterations in the freshwater clam, Caelatura nilotica (Cailliaud, 1827)

Sheir¹,

Mohamad²,

Osman³

et al. 2020

Egypt. J. of Aquatic Biolo. and Fish.

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Freshwater bivalves play important ecological roles, especially in shallow waters; rivers, lakes, and estuaries. Bivalves are widely used to assess environmental pollution because they respond with measurable physiological and morphological change. Additionally, they cannot escape pollution and therefore serve as sessile bioindicators. So, they can tolerate and accumulate high levels of heavy metals, pesticides and hormonal disrupting chemicals and then show signs of pathology (

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No substantial changes in estrogen receptor and estrogen-related receptor orthologue gene transcription in Marisa cornuarietis exposed to estrogenic chemicals

Cited by 35 publications

References 30 publications

The Nuclear Receptors of Biomphalaria glabrata and Lottia gigantea: Implications for Developing New Model Organisms

The Nuclear Receptors of Biomphalaria glabrata and Lottia gigantea: Implications for Developing New Model Organisms

Dynamics of nuclear receptor gene expression during Pacific oyster development

Acute and chronic effects of Bisphenol A on hormonal disruption and histological alterations in the freshwater clam, Caelatura nilotica (Cailliaud, 1827)

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