Does co-inoculation of Lactuca serriola with endophytic and arbuscular mycorrhizal fungi improve plant growth in a polluted environment?

Ważny, Rafał; Rozpądek, Piotr; Jędrzejczyk, Roman J.; Śliwa, Marta; Stojakowska, Anna; Anielska, Teresa; Turnau, Katarzyna

doi:10.1007/s00572-018-0819-y

Cited by 43 publications

(33 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence we speculate that the S. indica -conferred immune suppression might enhance the colonization of AMF in our experiment. This is supported by the fact that wild lettuce inoculated with both mycorrhizal and endophytic fungi enjoyed more benefits than that with a single fungus in a polluted environment ( Wazny et al, 2018 ). The mechanisms and degree of interaction and/or competition between R. irregularis and S. indica open new research opportunities for the future.…”

Section: Discussionmentioning

confidence: 99%

Beneficial Root Endophytic Fungi Increase Growth and Quality Parameters of Sweet Basil in Heavy Metal Contaminated Soil

et al. 2018

View full text Add to dashboard Cite

How interactions between plants, the rhizosphere, and contaminated soil affect environmental sustainability is still under research. We tested the effects of two root endophytic fungi, the arbuscular mycorrhiza fungus (AMF) Rhizophagus irregularis and the beneficial endophyte Serendipita indica, on sweet basil (Ocimum basilicum) growing on soil contaminated with lead and copper in a pot experiment under defined greenhouse conditions. Both fungi caused an increase in shoot and root dry weight of sweet basil plants under all conditions and decreased the amount of lead in shoots. The amount of copper was reduced by S. indica, while the AM fungus showed this effect only when the soil is contaminated with both copper and lead. Furthermore the AMF, but not the endophyte S. indica caused a strong increase on the concentrations of the essential oils linalool and eucalyptol even on sweet basil growing on contaminated soils. Hence, cultivating sweet basil in combination with beneficial fungi in case of difficult environmental conditions could be of interest for industry located in countries with widespread land pollution, because quantity and quality of plants are increased while the amount of heavy metals is generally reduced.

show abstract

Section: Discussionmentioning

confidence: 99%

Beneficial Root Endophytic Fungi Increase Growth and Quality Parameters of Sweet Basil in Heavy Metal Contaminated Soil

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The studies that have investigated the effect of DSE on AMF colonization have revealed two general patterns. Saravesi (2014) and Berthelot et al (2018) grown with Mucor species in uncontaminated soils (Ważny et al, 2018). Thus, the reports available suggest that endophytic fungi either have no effect or promote total AMF colonization, which is in agreement with the results I obtained for A. tridentata and P. secunda, respectively.…”

Section: Effects Of Dse Inoculation On Amf Colonizationsupporting

confidence: 89%

A Symbiosis Between a Dark Septate Fungus, an Arbuscular Mycorrhiza, and Two Plants Native to the Sagebrush Steppe

Carpenter¹

View full text Add to dashboard Cite

Plant roots form symbioses with various fungi, including arbuscular mycorrhizae (AMFs) and dark septate endophytes (DSEs). The symbiosis between plants and AMFs has been extensively studied and is generally considered to be mutualistic. Much less is known about the symbiosis between plants and DSE. In sagebrush habitats, DSEs are common, but their effects on the vegetation are unclear. As a first step to study these effects, I isolated and cultured a DSE from the roots of the shrub Artemisia tridentata. Based on partial sequences of five genes and phylogenetic analyses, the isolated fungus was a non-described species within the Darksidea or a closely related sister group. Subsequently, I performed experiments in vitro and in potted plants to determine the effect of the isolated DSE on root tissue integrity, colonization by the AMF Rhizophagus irregularis, and plant biomass. These experiments were conducted in two plant species, A. tridentata and the native grass Poa secunda. Plants were exposed to one of four treatments: no inoculation (-AMF-DSE), inoculation with the DSE isolate (-AMF+DSE), inoculation with R. irregularis (+AMF-DSE), and inoculation with both fungi (+AMF+DSE). Microscopic observations revealed that the DSE hyphae grew along the root surface and penetrated epidermal and cortical cells without damage to them. In A. tridentata, the hyphae also reached the stele. For both species, total DSE colonization in the –AMF+DSE treatment was similar to that in the +AMF+DSE treatment, indicating the presence of AMF did not alter DSE colonization. Inoculation with DSE did not affect total AMF colonization of A. tridentata; however, it increased total colonization of P. secunda from 16.9 (+5.6%) in the +AMF-DSE treatment to 42.6 (+2.9%) in the +AMF+DSE treatment. Also, in both species, the presence of the DSE more than doubled the frequency of AMF intraradical storage structures, which consisted of vesicles plus intraradical spores. These results suggest that via increases in AMF colonization, DSE could lead to a beneficial effect on the host plants. However, neither on its own nor through co-inoculation with AMF, did the DSE isolate affect plant biomass. Thus, under the two conditions tested, the symbiosis was commensalistic. Further work is needed to evaluate the symbiosis in settings that better mimic the natural environment.

show abstract

“…In our previous work, we showed that endophytic fungi positively affect the growth of their host under metal toxicity, low nutrient abundance, and limited water availability (Rozpądek, Domka, Ważny, et al ; Ważny et al, ). The aim of this study was to verify whether the benefits imposed by the fungus are solely stress related and how the symbiotic organism adapts to the increased carbohydrate demand, resulting from (a) the improved growth and (b) the presence of the microorganism, which is an additional sink for carbohydrates.…”

Section: Discussionmentioning

confidence: 99%

“…The plants were cultured in vitro for 10–14 days after inoculation. For the pot culture experiments, the seedlings were transferred to 80‐ml pots filled with a mixture of sterile commercial soil (ARO, PL: pH 5–6.5; N‐NO 3 , 100–300 mg L −1 ; P, 80–300 mg L −1 ; K, and 150–450 mg L −1 ) as described by Ważny et al () with sand in a 1:1 (v/v) ratio after 10 days of culture. Alternatively, 10 days after germination in the soil, the seedlings were inoculated with 0.8 ml of fungal inoculum (4 × 10 5 spores ml −1 ) suspended in water with 0.05% Tween‐80.…”

Section: Methodsmentioning

confidence: 99%

“…In our previous research, we showed that the endophytic Zygomycete Mucor sp. (closely related to Mucor racemosus and Mucor plumbeus ) isolated from Arabidopsis arenosa inhabiting a post‐mining dump facilitated vegetative growth when grown under metal toxicity and low nutrient abundance and in A. thaliana in vitro (Rozpądek, Domka, Nosek, et al, ; Rozpądek, Domka, Ważny, et al, ; Ważny et al, ). Interestingly, studies with Mucoromycotina fungi indicate that this plant–endophyte interaction may be older than the symbiosis between plants and mycorrhiza (Field et al, , ); thus, the interaction between Mucor sp.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Acclimation of the photosynthetic apparatus and alterations in sugar metabolism in response to inoculation with endophytic fungi

Rozpądek

Nosek

Domka

et al. 2018

Plant Cell & Environment

Self Cite

View full text Add to dashboard Cite

The role of an endophytic Zygomycete Mucor sp. in growth promotion and adaptation of the photosynthetic apparatus to increased energy demands of its hosts Arabidopsis arenosa and Arabidopsis thaliana was evaluated. Inoculation with the fungus improved the water use efficiency of the plants and allowed for them to utilize incident light for photochemistry more effectively by upregulating the expression of several photosystem I‐ and II‐related genes and their respective proteins, proteins involved in light harvesting in PSII and PSI and carbon assimilation. This effect was independent of the ability of the plants to acquire nutrients from the soil. We hypothesize that the accelerated growth of the symbiotic plants resulted from an increase in their demand for carbohydrates and carbohydrate turnover (sink strength) that triggered a simultaneous upregulation of carbon assimilation. Arabidopsis plants inoculated with Mucor sp. exhibited upregulated expression in several genes encoding proteins involved in carbohydrate catabolism, sugar transport, and smaller starch grains that indicate a significant upregulation of carbohydrate metabolism.

show abstract

Does co-inoculation of Lactuca serriola with endophytic and arbuscular mycorrhizal fungi improve plant growth in a polluted environment?

Cited by 43 publications

References 60 publications

Beneficial Root Endophytic Fungi Increase Growth and Quality Parameters of Sweet Basil in Heavy Metal Contaminated Soil

Beneficial Root Endophytic Fungi Increase Growth and Quality Parameters of Sweet Basil in Heavy Metal Contaminated Soil

A Symbiosis Between a Dark Septate Fungus, an Arbuscular Mycorrhiza, and Two Plants Native to the Sagebrush Steppe

Acclimation of the photosynthetic apparatus and alterations in sugar metabolism in response to inoculation with endophytic fungi

Contact Info

Product

Resources

About