Does CYP1A1 gene polymorphism affect cell damage biomarkers and ageing?

Eshkoor, Sima Ataollahi; Ismail, Patimah; Rahman, Sabariah Abdul

doi:10.3906/biy-1308-61

Cited by 3 publications

(5 citation statements)

References 41 publications

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“…In humans, since the CYP1A1 is an extrahepatic protein, it is considered to assume a secondary function in the removal of medications in vivo , and consequently, the polymorphism of the CYP1A1 gene may have a minor impact on their metabolic clearance. CYP1A1 assume essential tasks in the bio-activation of a collection of cancer-causing polycyclic aromatic hydrocarbons [PAHs; e.g ., benzo[a]pyrene (B[a]P)], aromatic amines and amides, and mycotoxins ascertained in certain grains, e.g ., aflatoxin B1[10,38-41]. CYP1A1 is also committed in the metabolism of endogenous compounds such as the pineal hormone melatonin and 17 β-estradiol and estrone[42-44].…”

Section: Cyp1a1mentioning

confidence: 99%

Role of cytochrome P450 polymorphisms and functions in development of ulcerative colitis

Sen

Stark

2019

WJG

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Cytochromes P450s (CYPs) are terminal enzymes in CYP dependent monooxygenases, which constitute a superfamily of enzymes catalysing the metabolism of both endogenous and exogenous substances. One of their main tasks is to facilitate the excretion of these substances and eliminate their toxicities in most phase 1 reactions. Endogenous substrates of CYPs include steroids, bile acids, eicosanoids, cholesterol, vitamin D and neurotransmitters. About 80% of currently used drugs and environmental chemicals comprise exogenous substrates for CYPs. Genetic polymorphisms of CYPs may affect the enzyme functions and have been reported to be associated with various diseases and adverse drug reactions among different populations. In this review, we discuss the role of some critical CYP isoforms (CYP1A1, CYP2D6, CYP2J2, CYP2R1, CYP3A5, CYP3A7, CYP4F3, CYP24A1, CYP26B1 and CYP27B1) in the pathogenesis or aetiology of ulcerative colitis concerning gene polymorphisms. In addition, their significance in metabolism concerning ulcerative colitis in patients is also discussed showing a clear underestimation in genetic studies performed so far.

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Section: Cyp1a1mentioning

confidence: 99%

Role of cytochrome P450 polymorphisms and functions in development of ulcerative colitis

Sen

Stark

2019

WJG

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“…DNA damage effects of the used substances were confirmed in mechanical workshops workers, but with no confirmation of the influence of GSTP1 [66] or CYP1A1 [69] gene polymorphism on DNA damage, considering the comet assay performed on buccal cells. Conversely, workers with the wild genotype for CYP2E1 showed statistically significant higher comet tail length at the occupational exposure, while the mutated genotype did not have influence on this biomarker [67].…”

Section: Life Style Dietary Genetic and Healthy Factorsmentioning

confidence: 90%

“…The comet assay in buccal cells has been used to evaluate DNA damage induced by different materials such as mouthrinses [50], metals released from orthodontic appliances [51][52][53][54][55][56][57][58][59], ionizing radiation [60], as well as assessment of DNA damage, and its modulation by life-style, dietary, genetic and healthy factors [61][62][63][64][65][66][67][68][69][70][71][72][73][74], occupational exposure [66][67][68][69][75][76][77][78][79][80][81][82], and environmental exposure [83][84][85][86]. Different procedures have been used in collecting and processing the samples that are presented and discussed in Rojas et al [33].…”

Section: Use Of Comet Assay In Buccal Cellsmentioning

confidence: 99%

“…Tobacco-related compounds cause a variety of DNA damage, and DNA repair capacity plays an important role in agent-induced damage genotoxic. Several polymorphisms in genes that participate in different DNA repair pathways, such as XRCC1 399, hOGG1 326 [65], GSTP1 [66], CYP2E1 [67], CYP1A2 [68], and CYP1A1 [69], have been evaluated for their effects on different biomarkers [89], including comet tail length in buccal cells.…”

Section: Life Style Dietary Genetic and Healthy Factorsmentioning

confidence: 99%

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Assessment of DNA Damage by Comet Assay in Buccal Epithelial Cells: Problems, Achievement, Perspectives

Sánchez-Alarcón¹,

Milić²,

Gómez‐Arroyo³

et al. 2016

Environmental Health Risk - Hazardous Factors to Living Species

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DN" damage risk assessment in comet assay by the use of buccal mucosa cells has great advantages in comparison with other cell type sample due to more safely, easier, cheaper, and non-invasive method for in vivo studies. "ccording to the OECD Guidelines, the in vivo mammalian alkaline comet assay is well-established and validated method for measuring DN" strand breaks in single eukaryotic cells. Considering exposure to xenobiotics and endogenous damage inductors, buccal mucosa cells are the first to be in direct contact after exposure and this makes them an ideal biomatrices in evaluation of the level of individual genotoxicity to several compounds already mentioned. Their clinical diagnostic applicability confers a potential use in patients across time. However, the number of publications referring to the human buccal comet assay is low in the last two decades. This low growing interest may be explained by several factors, including its relative technical problems. Different procedures have been used in collecting and processing the samples. In order to have widespread acceptance and credibility in human population studies, the comet assay in buccal cells requires standardization of the protocol, of parameters analyzed, and a better knowledge of critical features affecting the assay outcomes, including the definition of the values of spontaneous DN" damage. There is a need for further collaborative work as in the HUMN micronucleus assay on lymphocytes and HUMNxL micronucleus assay on buccal cells collaborative projects. The creation of a network of laboratories will allow more focused validation studies, including the design of a classic, historic, prospective cohort study in order to explore the link between measures of genetic instability in the buccal mucosa and the risk of cancer and other chronic-degenerative diseases. One such network connection will start in as a COST project under the name hCOMET The comet assay as a human biomonitoring tool launched by Prof. "ndrew Collins.

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“…The increased cost and time requisite for screening the effect of mutation by conventional laboratory-based experimentations is impossible due to the presence of enormous numbers of SNPs. However, information on mutated gene function is essentially required, and particularly on missense mutations causing hereditary diseases (Eshkoor et al, 2014). The understanding of the relationships between protein structure and function is also a prerequisite.…”

Section: Introductionmentioning

confidence: 99%

Identification and prediction of the consequences of nonsynonymous SNPs in glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene of zebrafish Danio rerio

Rasal¹,

Chakrapani²,

Patra³

et al. 2016

Turk J Biol

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The recent progress in DNA sequencing and computational algorithms dramatically heightened the value of single nucleotide polymorphism (SNP) databases. The experimental studies of mutation for the particular gene of interest are laborious and timeconsuming. In this study, we used several computational algorithms to investigate the structural and functional consequences of SNPs on the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene of zebrafish (Danio rerio). The GAPDH gene is involved in multiple cellular activities, in addition to its association with classical glycolytic pathway. It has most commonly been used as the housekeeping gene for differential gene expression profiling, including western blots. The computational analysis, which used sequence-based tools such as SIFT, PANTHER, PROVEAN, and I-Mutant2.0, predicted deleterious nonsynonymous SNPs (nsSNPs) at p.W194R in the GAPDH gene. The SWISS Model tool generated a homology-modeled 3D structure of GAPDH by applying homologous protein structures retrieved from the RCSB PDB databank (PDB ID: 1U8F_O, 1ZNQ_O, 1J0X_O, 3GPD_R, and 1SZJ_G). The sequence identities of the above selected models were 86%, 86%, 86%, 83%, and 75%, respectively. The Procheck quality assessment in the Ramachandran plot showed that the incorporated mutation (p.W194R) led to a shifting of amino acid residues from the most favored regions towards the disallowed region, as compared to the native counterpart. The lowered ERRAT and PROSA Z scores in the mutant confirmed the overall deteriorating quality of mutant GAPDH. We also predicted an effect of mutation on increased H-bonding patterns due to mutant residue. The molecular docking analysis, while binding GAPDH to its ligand (NAD +), also revealed elevated global energy in the mutant. Altogether, this study demonstrated the impact of nsSNPs on the protein structure and function. The results of work provide an insight/ roadmap for future investigations with regard to the pathological consequences of nsSNPs using in vivo methods.

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Does CYP1A1 gene polymorphism affect cell damage biomarkers and ageing?

Cited by 3 publications

References 41 publications

Role of cytochrome P450 polymorphisms and functions in development of ulcerative colitis

Role of cytochrome P450 polymorphisms and functions in development of ulcerative colitis

Assessment of DNA Damage by Comet Assay in Buccal Epithelial Cells: Problems, Achievement, Perspectives

Identification and prediction of the consequences of nonsynonymous SNPs in glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene of zebrafish Danio rerio

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