Does dikegulac affect in vitro shoot proliferation  and hyperhydricity incidence in olive explants?

Antonopoulou, Chrysovalantou; Dimassi, K.; Therios, Ioannis; Chatzissavvidis, Christos

doi:10.17221/212/2016-hortsci

Cited by 6 publications

(4 citation statements)

References 32 publications

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“…BA (6-benzyladenine) was comparable to zeatin for shoot regeneration from nodal explants of the Olive “Gemlik” [ 1 ]. The use of a combination of BA μΜ dikegulac enhanced shoot proliferation of olive tree cv “Chondrolia Chalkidikis” and the production of new shoots of good quality, diminishing vitrification symptoms [ 20 ]. Additionally, BAP (6-benzylaminopurine) and 2iP (dimethyl-allyl-aminopurine) were tested but, on some cultivars, produced results inferior to zeatin at the end of proliferation [ 21 ] or, as in the case of BAP, provided the best results in combination with zeatin [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Neem Oil to Reduce Zeatin Use and Optimize the Rooting Phase in Olea europaea L. Micropropagation

Regni

Facchin

Silva

et al. 2023

Plants

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Micropropagation is an in vitro propagation technique, established in the nursery field sector for numerous species, which offers several advantages compared to traditional agamic propagation techniques. In the case of the olive tree, however, despite the advances made through research, it is still little used, due to the recalcitrance to in vitro proliferation and/or rooting of many olive cultivars and the high cost of zeatin, the only cytokinin that makes it possible to achieve a satisfactory proliferation rate in this species. In this context, numerous attempts have been made to identify alternative cytokinin compounds able to improve the proliferation rate of olive tree explants and thus reduce the unitary production cost. In particular, there is a growing interest in the use of natural substances (called in some cases “complex mixtures”), which, when added to the in vitro cultivation substrates, seem to be able to improve proliferation rates. In the present study, neem oil was added to the propagation substrates (partially/totally replacing zeatin) and in the rooting phase for the olive cultivar Moraiolo. In particular, in the proliferation phase, the effect of neem oil (0.1 mL L−1) in substrates containing different zeatin concentrations (0, 1, 2, and 4 mg L−1) was evaluated. For the rooting phase, agarized substrate and soil were used with shoots derived from a standard proliferation substrate (4 mg L−1 zeatin) and from the substrate that gave the best results in the proliferation phase (2 mg L−1 zeatin and 0.1 mL L−1 neem oil). In the proliferation phase, the addition of neem oil in the substrates with low zeatin concentration (1 and 2 mg L−1) induced an increase in the number of adventitious shoots and shoots length. On the contrary, the addition of neem oil in the rooting substrates did not positively influence the rooting phase, but positive results especially in terms of root number and length were observed in explants derived from a neem oil-enriched proliferation substrate compared to the control substrate. Therefore, the present study demonstrated for the first time the positive role of neem oil in the proliferation of olive in vitro with low zeatin concentrations.

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Section: Introductionmentioning

confidence: 99%

Neem Oil to Reduce Zeatin Use and Optimize the Rooting Phase in Olea europaea L. Micropropagation

Regni

Facchin

Silva

et al. 2023

Plants

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“…Hyperhydricity (vitrification) is one of the problems encountered in micropropagation. Vitrified shoots have smaller internodes and poor rooting ability [ 64 ]. No hyperhydricity was observed during our tests.…”

Section: Resultsmentioning

confidence: 99%

Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study

Gianguzzi

Barone

Gristina

et al. 2023

Plants

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The conservation of endangered, rare, and endemic plant species is based on in situ and ex situ conservation strategies. When in situ conservation alone is not sufficient to guarantee the survival of the species, ex situ techniques are adopted in support. This study aimed to develop an efficient micropropagation protocol for Adenostyles by evaluating the effect of different plant growth regulators on leaf explants. Adenostyles alpina subsp. macrocephala (Asterace) is a perennial herbaceous plant endemic to Calabria (Southern Italy). The genus Adenostyles includes three species confined to the mountains of the Mediterranean and southern Europe. For callus induction, media supplemented with different concentrations of Benzylaminopurine (BAP) (0.5, 1, 2, and 3 mg L−1), Naphthaleneacetic Acid (NAA) (1 mg L−1), and 2,4-Dichlorophenoxyacetic Acid (2,4-D) (1 mg L−1) were tested. Shoot regeneration and proliferation were obtained in media supplemented with BAP (1, 2, and 3 mg L−1) and NAA (1 mg L−1). Root induction was obtained in media supplemented with IBA (0.25, 0.50, and 1 mg L−1) and NAA (0.25, 0.50, and 1 mg L−1). Statistically significant differences in callus induction and shoot regeneration were observed between the various media tested. The medium containing Murashige and Skoog (MS) supplemented with 3 mg L−1 of BAP and 1 mg L−1 of NAA showed the highest percentage of callus induction and increased shoot regeneration. The regenerated shoots showed more effective root induction in the hormone-free MS medium and in the presence of Indole-3-Butyric Acid (IBA) at concentrations of 0.25, 0.50, and 1 mg L−1. These results can be used as a basis for the preparation of a micropropagation protocol for different taxa of Adenostyles, as well as other species of Asteraceae specialized to the Mediterranean mountain habitat.

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“…Moreover, it is a naturally occurring compound and very economical. Antonopoulou et al, 2018 studied the use of Dikegulac on olive intensive proliferation and found that it is not phytotoxic and much cheaper than zeatin. Sweeta et al, 2019 determined the effect of combination of coconut water, cytokinin and light intensity for enhanced direct in vitro organogenesis of olives (Table 3).…”

Section: Shoot Proliferationmentioning

confidence: 99%

Techniques for Micropropagation of Olive (Olea europaea L.): A Systematic Review

Haq¹,

Umar²,

Akhtar³

et al. 2021

PJAR

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Does dikegulac affect in vitro shoot proliferation and hyperhydricity incidence in olive explants?

Cited by 6 publications

References 32 publications

Neem Oil to Reduce Zeatin Use and Optimize the Rooting Phase in Olea europaea L. Micropropagation

Neem Oil to Reduce Zeatin Use and Optimize the Rooting Phase in Olea europaea L. Micropropagation

Micropropagation of Endemic Endangered Taxa of the Italian Flora: Adenostyles alpina subsp. macrocephala (Asteraceae), as a Case Study

Techniques for Micropropagation of Olive (Olea europaea L.): A Systematic Review

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