Does Maternal Treatment with Zidovudine Affect Changes in Mandibles of Newborns? Laser Induced Fluorescence Study

Abstract

The influence of antiretroviral drug zidovudine treatment during pregnancy on mandible development in newborn rats was studied. The fluorescence of mandibles from 7-, 14- and 28-days old individuals was measured by means of fiber-optical fluorescence analyzer with 407 nm laser excitation. Obtained results revealed disturbing effect of maternal zidovudine administration on mandible fluorescence intensity which should decrease with bone development. Small changes in fluorescence of porphyrin forms are maintaining in the first month of newborns life while the changes observed in 440-585 nm range disappear.

The changes in bone organic and inorganic matrix in newborn rats after maternal application of antiretroviral agents: Indinavir and zidovudine

“…One can see a broad peak between 410 and 700 nm with its maximum at 500 nm with some right-sided asymmetry. These spectra are substantially similar to those reported earlier [12]. It seems that LIF fluorescence in our experiment results mainly from endogenous fluorophores in bones: lipids with maximum peak of emission at 450 to 660 nm, FAD and FMN with maximum peak of emission at 465 to 535 nm and vitamins, mainly vitamin D with maximum peak of emission at 480 nm.…”

“…The penetration depends on tissue type, which is additionally decreased in bones due to its high absorption . All the technical data and measurement conditions were described earlier . LIF spectra from bone meal as well as inorganic KH‐2 limestone were taken in order to check whether the obtained LIF fluorescence did not come from inorganic matrix.…”

“…All the technical data and measurement conditions were described earlier [12]. LIF spectra from bone meal as well as inorganic KH-2 limestone were taken in order to check whether the obtained LIF fluorescence did not come from inorganic matrix.…”

Effect of Endurance Training and Testosterone Treatment on the Fluorescence Spectra of Rat Serum

“…Many efforts have been made to optimize the detection and imaging of diseased tissues, exploring the diagnostic potential of exogenous fluorophores developed for photodynamic diagnosis and therapy [8]. Fluorescence spectroscopy may also give valuable information in non-cancerous cases, for example, fluorescence spectroscopy was applied as a tool for bone development monitoring in new-born rats [9,10].…”

An Assessment of the Impact of Zidovudine on Rat Teeth Ontogenesis with Quantitative Laser Induced Fluorescence and an Histomorphological Analysis

“…Knowledge of the status of the above characteristics of an organism helps ensure early detection of subclinical pathologies, including early detection of premalignant and neoplastic changes [10,[15][16][17][18][19][20] and differential diagnosis of different types of pathological changes [9,19]. It can also be used to assess the impact of drugs on tissues [21,22], distinguish between healthy body tissues and dysplastic lesions [19], evaluate tumour lesion demarcation [19,23,24] and provide early detection of initial caries in teeth [12,[24][25][26][27]. Apart from its possible application in diagnostics, fluorescence also serves as an important guide in longterm evaluations and monitoring of spontaneous or treatment-induced changes in tissues, including as a method for monitoring the carious spot in teeth and its remineralisation [12,13,[24][25][26][27][28][29][30][31], monitoring the effects of antibiotic therapy and phototherapy in bacteriological skin diseases [13], as well as in photodynamic treatment (PDT) of neoplasms [28] and precancerous conditions [20,29].…”