Does prolonged pneumoperitoneum affect the kidney? Oxidative stress, stereological and electron microscopy study in a rat model

Souza, Diogo Benchimol de; Costa, Waldemar S.; Cardoso, Luiz E.M.; Benchimol, Marlene; Pereira‐Sampaio, Marco A.; Sampaio, Francisco J.B.

doi:10.1590/s1677-5538.ibju.2013.01.05

Cited by 19 publications

(27 citation statements)

References 29 publications

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“…Stereological methods have been used to determine the glomerular number in kidneys affected by pneumoperitoneum, diabetes, and chronic stress 19,22,26 . Stereological glomeruli quantification has also been used to study renal damage after partial nephrectomy 14 or for comparing arteriovenous with arterial renal ischemia 13 .…”

Section: ■ Discussionmentioning

confidence: 99%

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The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig model

et al. 2017

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The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig model 1 Acta Cir. Bras. 2017;32(5):334-341 Abstract Purpose: To investigate the glomerular number after different warm ischemia times. Methods: Thirty two pigs were assigned into four groups. Three groups (G10, G20, and G30) were treated with 10, 20, and 30 minutes of left renal warm ischemia. The sham group underwent the same surgery without renal ischemia. The animals were euthanized after 3 weeks, and the kidneys were collected. Right kidneys were used as controls. The kidney weight, volume, cortical-medullar ratio, glomerular volumetric density, volume-weighted mean glomerular volume, and the total number of glomeruli per kidney were obtained. Serum creatinine levels were assessed pre and postoperatively. Results: Serum creatinine levels did not differ among the groups. All parameters were similar for the sham, G10, and G20 groups upon comparison of the right and left organs. The G30 group pigs' left kidneys had lower weight, volume, and cortical-medullar ratio and 24.6% less glomeruli compared to the right kidney. A negative correlation was found between warm ischemia time and glomerular number. Conclusions: About one quarter of glomeruli was lost after 30 minutes of renal warm ischemia. No glomeruli loss was detected before 20 minutes of warm ischemia. However, progressive glomerular loss was associated with increasing warm ischemia time.

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Section: ■ Discussionmentioning

confidence: 99%

“…The specimens were paraffin embedded, sectioned at 5-µm thickness, and stained by hematoxylin & eosin. The cortical-medullar ratio was estimated by using the point-counting-method according the Cavalieri principle 18,19 . The absolute cortical volume (CV) was calculated by multiplying the cortical-medullar ratio by the renal volume.…”

Section: ■ Introductionmentioning

confidence: 99%

The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig model

et al. 2017

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“…The cortical-medullar ratio was estimated by using the Cavalieri principle 15 and the absolute cortical volume (CV) was calculated by multiplying the cortical-medullary ratio by the renal volume.…”

Section: Methodsmentioning

confidence: 99%

“…The estimation of the total number of glomeruli per kidney was calculated by multiplying the CV by the Vv [glom] and dividing the result by the VWGV 14,15 .…”

Section: Methodsmentioning

confidence: 99%

Glomerular loss after arteriovenous and arterial clamping for renal warm ischemia in a swine model

et al. 2016

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PURPOSE:To evaluate the glomerular loss after arteriovenous or arterial warm ischemia in a swine model. METHODS:Twenty four pigs were divided into Group Sham (submitted to all surgical steps except the renal ischemia), Group AV (submitted to 30 minutes of warm ischemia by arteriovenous clamping of left kidney vessels), and Group A (submitted to 30 minutes of ischemia by arterial clamping). Right kidneys were used as controls. Weigh, volume, cortical volume, glomerular volumetric density (Vv[Glom]), volume-weighted glomerular volume (VWGV), and the total number of glomeruli were measured for each organ. RESULTS:Group AV showed a 24.5% reduction in its left kidney Vv[Glom] and a 25.4% reduction in the VWGV, when compared to the right kidney. Reductions were also observed when compared to kidneys of sham group. There was a reduction of 19.2% in the total number of glomeruli in AV kidneys. No difference was observed in any parameters analyzed on the left kidneys from group A. CONCLUSIONS:Renal warm ischemia of 30 minutes by arterial clamping did not caused significant glomerular damage, but arteriovenous clamping caused significant glomerular loss in a swine model. Clamping only the renal artery should be considered to minimize renal injury after partial nephrectomies.

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“…The rats were randomly divided into two groups: a Sham group (n = 7), which was submitted only to anesthesia for 210 minutes, and a pneumoperitoneum (Pp) group (n = 7) which underwent to a 180 minutes of Pp at 8 mmHg followed by 30 minutes of desufflation, under the same anesthetic protocol of the Sham group 10 . All rats were killed six weeks after the procedure and their testes were used for seminiferous tubule diameter, seminiferous tubule epithelium height analyses and Johnsen`s score.…”

Section: Methodsmentioning

confidence: 99%

Pneumoperitoneum induces morphological alterations in the rat testicle

et al. 2013

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PURPOSE:To investigate the seminiferous tubule histological morphology after an 8 mmHg pneumoperitoneum in the rat model. METHODS:Fourteen rats were divided into two groups: a Sham group submitted to anesthesia and a pneumoperitoneum (Pp) group submitted to abdominal insufflation at 8 mmHg during three hours, followed by desuflation. All rats were killed after six weeks, testicles were collected and evaluated for the tubule diameter, germinative epithelium height and Johnsen´s score. Means were compared by using the Student's-t-test. RESULTS:The seminiferous tubule diameter was diminished by 11.3% in the group submitted to pneumoperitoneum (p<0.05). No significant difference was found among the groups when analyzing the epithelium height and Johnsen´s score. CONCLUSION:In the rat model, the seminiferous tubules present structural alterations when subjected to pneumoperitoneum of 8 mmHg during three hours.

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Does prolonged pneumoperitoneum affect the kidney? Oxidative stress, stereological and electron microscopy study in a rat model

Cited by 19 publications

References 29 publications

The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig model

The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig model

Glomerular loss after arteriovenous and arterial clamping for renal warm ischemia in a swine model

Pneumoperitoneum induces morphological alterations in the rat testicle

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