Does selenium improve the stem cell therapeutic effect on isoproterenol-induced myocardial infarction in rats? A histological and immunohistochemical study

Salem, Mahmoud; El-Azab, Nahla El-Eraky; Helal, Omayma K.; Gabr, Hala; Bayoumi, Heba

doi:10.1097/01.ehx.0000475224.41506.75

Cited by 5 publications

(7 citation statements)

References 37 publications

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“…There were mild to moderate immunoreactivity in neck region to PCNA approximately similar to the control negative group and this is as a result of the ability of MSCs to regenerate damaged tissue and normalize the proliferation process after re-epithelialisation, differentionand success in gastric ulcer healing and this came in agreement with Salem et al (2015) .…”

Section: Discussionsupporting

confidence: 73%

Role of bone marrow derived-mesenchymal stem cells against gastric ulceration: Histological, immunohistochemical and ultrastructural study

Alazzouni

Fathalla

Gabri

et al. 2020

Saudi Journal of Biological Sciences

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The current study aimed to assess the antiulcerogenic impact of mesenchymal bone marrow stem cells (BMMSCs) against gastric ulcer induced by the use of piroxicam in rats and to compare this effect with the antiulcer drug “Pantoloc ®” proton pump inhibitors. The study included histological, histochemical, immunohistochemical and ultrastructural examination in stomach of rats in different study groups. In the ulcerated group, the glandular region of the stomach displayed clear mucosal lesions occurring as perforations along the stomach axis. In addition, stomach displayed degeneration of surface mucous cells accompanied by pyknosis, vacuolation among parietal cells in ishmus region, basal region with vacuolated chief cells and karyolitic nucleus of parietal cells. Moreover, Stomach sections of ulcer model rats showed intensive immunoreactivity to cytokeratin 20, Cox 2 and PCNA. Findings of the present study have shown that BMMSCs have an ameliorative effect against piroxicam-induced gastric ulcer in rats. Collectively, the proposed work has shown that BMMSCs have a curative capacity as an antiulcer due to their high antioxidant activity. Further studies are required in molecular levels to understand the mechanism of action during treatment.

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Section: Discussionsupporting

confidence: 73%

Role of bone marrow derived-mesenchymal stem cells against gastric ulceration: Histological, immunohistochemical and ultrastructural study

Alazzouni

Fathalla

Gabri

et al. 2020

Saudi Journal of Biological Sciences

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“…The effectiveness of stem cell treatments mainly depends on suitable control of the role and death of the transplanted cells. So, the policies for improving stem cell survival, proliferation and differentiation considered as the most interesting research points [14] .…”

Section: Discussionmentioning

confidence: 99%

“…At the place of injury, cells experience oxidative and inflammatory stresses, which could lead to cellular aging and apoptosis. Thus, rising of the cellular antioxidant levels could expand the survival rate of stem cells and therefore, promotes the functional repair [14] .…”

Section: Discussionmentioning

confidence: 99%

“…Mesenchymal stem cells (MSCs) provide a promising alternative approach for treating human diseases. They primarily originate from the mesoderm and the ectoderm during early embryonic development and are present in several types of tissues and organs as bone marrow, fat, muscles, lungs, liver, pancreas, and synovial membrane [2,14,15] . MSCs are of great values on clinical proposal because of their easy isolation, multipotency, and high proliferative potential invitro.…”

Section: Introductionmentioning

confidence: 99%

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Comparative study of the therapeutic effect of pomegranate alone or in combination with bone marrow mesenchymal stem cells on experimentally induced gastric ulcer in adult male rats: A histological and immunohistochemical study

El-Azab

Mansy

El-Mahalaway

et al. 2018

Egyptian Journal of Histology

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Introduction: Gastric ulcers are the most prevalent gastrointestinal disorders. Pomegranate fruits have many beneficial therapeutic effects. Bone marrow mesenchymal stem cells (BMSCs) are a novel approach with promising therapeutic potential for curing human diseases. Aim of the work: To evaluate the effect of pomegranate and BMSCs on experimentally induced gastric ulcer in adult male rats. Materials and Methods: Sixty adult male rats were divided into four groups. Group I kept as the control group. Group II (Gastric ulcer), rats received 100% ethanol orally by gastric tube. Group (III) subdivided into 3 subgroups. Subgroup IIIa included rats with gastric ulcer that treated with pomegranate only. Subgroup IIIb, rats with gastric ulcer treated with BMSCs alone. Subgroup IIIc; gastric ulcer rats treated with a combination of pomegranate and BMSCs. Group IV included the untreated gastric ulcer rats. Fundus of stomach specimens were prepared and examined using histological and immunohistochemical techniques. Results: Groups II and IV showed various changes as deep erosion, marked damage of most of the cells. The presence of the inflammatory cells in gastric mucosa were supported by a significant increase of tumor necrosis factor α (TNF-α) expression and collagen fibers depositions (P<0.01). A significant decrease of Periodic Acid Schiff (PAS) positive reaction and vascular endothelial growth factor (VEGF) expression was observed (P<0.01). Subgroup IIIa showed attenuation of some histological changes, while subgroups IIIb and IIIc revealed more improvement of the histological and immunohistochemical changes described before. Conclusion: BMSCs can ameliorate experimentally-induced gastric injury in rats and protect the gastric tissue. Addition of pomegranate to BMSCs can lead to better results.

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“…MSCs originate from the mesoderm and the ectoderm throughout early embryonic development and are found in several kinds of tissues and organs [10,11]. They form a promising therapeutic line for neurodegenerative diseases.…”

Section: Introductionmentioning

confidence: 99%

Effect of Methyl Mercury on the Cerebellar Cortex of Rats and the Possible Neuroprotective Role of Mesenchymal Stem Cells Conditioned Medium. Histological and Immunohistochemical Study

El-Azab¹,

El-Mahalaway²,

Sabry³

2018

J Stem Cell Res Ther

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Background: Methyl mercury (Me Hg) is an environmental toxin associated with many serious neurological disorders. Conditioned Medium (CM) derived from Mesenchymal Stem Cells (MSCs) is a novel promising approach for the treatment of nervous system damage and various diseases. Objective: The aim of this research is to assess the consequence of Me Hg on the rats' cerebellar cortex and the potential neuroprotective effect of MSCs-CM. Materials and methods: Forty adult male albino rats were divided into four groups: Group I: control rats; Group II: Me Hg chloride treated rats; Group III: Me Hg chloride and DMEM treated rats; Group IV: CM treated rats after injection with Me Hg chloride. Cerebellar specimens were taken and handled for histological and immunohistochemical techniques. Morphometrical studies and statistical analysis were performed. Results: Groups II and III showed various changes such as neuronal degeneration and apoptosis. The mean number of Purkinje cells was significantly decreased(P<0.01), while Glial Fibrillary Acidic Protein (GFAP) immunostaining was significantly increased (P<0.01) in the neuroglial cells. Ultrastructural examination showed thinning and apparent decrease in the number of myelinated nerve axons. Shrunken Purkinje cells were observed with irregular nuclei, heterogenous cytoplasm, and disrupted mitochondria. Group IV showed improvement of the histological and electron microscopic changes defined in groups II and III. Conclusion: Me Hg exposure led to degenerative changes on cerebellar cortex. MSCs-CM is a very promising approach and has neuroprotective effects.

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Does selenium improve the stem cell therapeutic effect on isoproterenol-induced myocardial infarction in rats? A histological and immunohistochemical study

Cited by 5 publications

References 37 publications

Role of bone marrow derived-mesenchymal stem cells against gastric ulceration: Histological, immunohistochemical and ultrastructural study

Role of bone marrow derived-mesenchymal stem cells against gastric ulceration: Histological, immunohistochemical and ultrastructural study

Comparative study of the therapeutic effect of pomegranate alone or in combination with bone marrow mesenchymal stem cells on experimentally induced gastric ulcer in adult male rats: A histological and immunohistochemical study

Effect of Methyl Mercury on the Cerebellar Cortex of Rats and the Possible Neuroprotective Role of Mesenchymal Stem Cells Conditioned Medium. Histological and Immunohistochemical Study

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