Does single-dose cell resistance to the radio-mimetic zeocin correlate with a zeocin-induced adaptive response in Chlamydomonas reinhardtii strains?

Dimova, Emiliya; Dimitrova, Margarita; Miteva, Daniela A.; Mitrovska, Zhana; Yurina, N. P.; Bryant, Peter E.; Chankova, Stephka

doi:10.1007/s00411-008-0199-3

Cited by 17 publications

(12 citation statements)

References 35 publications

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“…Despite abundant literature available on the action of bleomycin [ 16 , 17 ], studies on the effect of Zeo have been focused on Zeo as causing transformants in various test-systems [ 18 , 19 , 20 ]. Data on the DNA-damaging action of Zeo in only one plant system has been reported [ 13 , 14 ]. Therefore the second step of this study was to evaluate DNA-damaging (genotoxic) potential of Zeo over a wider concentration range.…”

Section: Resultsmentioning

confidence: 99%

“…The effects of zeocin (Zeo) on the DNA are detrimental for cells. Zeo, as a member of bleomycin/phleomycin family of glycopeptide antibiotics, is known to bind to DNA and induce oxidative stress in different organisms, like ionizing radiation, producing predominantly single and double strand breaks (SSBs/DSBs), as well as base losses [ 13 , 14 ]. Many defense mechanisms have been evolved to minimalize such effects of oxidative stress, including the AR.…”

Section: Introductionmentioning

confidence: 99%

“…Many defense mechanisms have been evolved to minimalize such effects of oxidative stress, including the AR. Thus, in the green alga Chlamydomonas reinhardtii Zeo, as a radiomimetic, has been shown to induce an AR [ 14 ]. Chankova et al [ 13 ] concluded that Zeo-induced AR in C. reinhardtii was a result of the induced acceleration of the rejoining of DSBs.…”

Section: Introductionmentioning

confidence: 99%

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Extract of Lillium candidum L. Can Modulate the Genotoxicity of the Antibiotic Zeocin

et al. 2011

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Lilium candidum L. extract (LE) is well known in folk medicine for the treatment of burns, ulcers, inflammations and for healing wounds. This work aims to clarify whether the genotoxic potential of the radiomimetic antibiotic zeocin (Zeo) could be modulated by LE. Our results indicate that LE exerts no cytotoxic, DNA-damaging and clastogenic activity in in Chlamydomonas reinhardtii, Pisum sativum L. and Hordeum vulgare L. test systems over a broad concentration range. Weak but statistically significant clastogenic effects due to the induction of micronuclei and chromosome aberrations have been observed in H. vulgare L. after treatment with 200 and 300 μg/mL LE. To discriminate protective from adverse action of LE different experimental designs have been used. Our results demonstrate that the treatment with mixtures of LE and Zeo causes an increase in the level of DNA damage, micronuclei and “metaphases with chromatid aberrations” (MwA). Clear evidence has been also obtained indicating that pretreatment with LE given 4 h before the treatment with Zeo accelerates the rejoining kinetics of Zeo-induced DNA damage in P. sativum L. and C. reinhardtii, and can decrease clastogenic effect of Zeo measured as frequencies of micronuclei and MwA in H. vulgare L. Here, we show for the first time that LE can modulate the genotoxic effects of zeocin. The molecular mode of action strongly depends on the experimental design and varies from synergistic to protective effect (adaptive response–AR). Our results also revealed that LE-induced AR to zeocin involves up-regulation of DSB rejoining in C. reinhardtii and P. sativum L. cells.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Extract of Lillium candidum L. Can Modulate the Genotoxicity of the Antibiotic Zeocin

et al. 2011

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“…The radiomimetic zeocin (Zeo) (Cas No. : 11006-33-0, Invi-troGen), which belongs to the bleomycin family of antibiotics, was used as a standard mutagenic factor, well investigated and described in our previous studies (Chankova et al, 2007;Dimova et al, 2009;Kopaskova et al, 2012).…”

Section: Standard Mutagenmentioning

confidence: 99%

Liliumcompounds kaempferol and jatropham can modulate cytotoxic and genotoxic effects of radiomimetic zeocin in plants and human lymphocytesIn vitro

2014

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Organisms are constantly exposed to the detrimental effect of environmental DNA-damaging agents. The harmful effects of environmental genotoxins could be decreased in a viable way by antimutagenesis. One of the modern approaches to reduce the mutagenic burden is based on exogenous natural and synthetic compounds that possess protective and antimutagenic potential against genotoxins. The natural compounds kaempferol and jatropham isolated from Lilium candidum were tested with respect to their potential to protect cells against the radiomimetic zeocin, as well as to their cytotoxic and genotoxic activities in two types of experimental eukaryotic test systems: Hordeum vulgare and human lymphocytes in vitro. Mitotic index (MI) was used as an endpoint for cytotoxicity; the frequency of chromosome aberrations (MwA) and the number of induced micronuclei (MN), as endpoints for genotoxicity/clastogenicity. Formation of aberration "hot spots" was also used as an indicator for genotoxicity in H. vulgare. Both kaempferol and jatropham were shown to possess a potential to modulate and decrease the cytotoxic and genotoxic/clastogenic effect of zeocin depending on the experimental design and the test system. Our data could be useful for health research programs, particularly in clarifying the pharmacological potential and activity of natural plant compounds. © 2014 Wiley Periodicals, Inc. Environ Toxicol 31: 751-764, 2016.

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“…Growing evidences have shown that the cell responses to ionizing radiation through genes associated with DNA repair, stress response, cell cycle control and apoptosis. TP-53 plays important roles in control of the low-dose radioadaptive response [ 2 , 6 , 7 ]. The microarray analysis performed by Lanza et al has shown that 111 genes are modulated at different doses of irradiation.…”

Section: Introductionmentioning

confidence: 99%

Ionizing Radiation-Induced Adaptive Response in Fibroblasts under Both Monolayer and 3-Dimensional Conditions

et al. 2015

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To observe the adaptive response (AR) induced by ionizing radiation in human fibroblasts under monolayer and 3-dimensional (3-D) condition. Three kinds of fibroblasts were cultured under both monolayer and 3-D condition. Immunofluorescent staining was used to detect the γ-H2AX foci and the morphological texture. Trypan blue staining was used to detect the cell death. Western blot was used to detect the expressions of γ-H2AX, p53 and CDKN1A/p21 (p21). We found that DNA damage increased in a dose-dependent and time-dependent manner after high doses of radiation. When cells were pretreated with a priming low dose of radiation followed by high dose radiation, DNA damage was attenuated under both monolayer and 3-D condition, and the adaptive response (AR) was induced. Additionally, the morphology of cells under monolayer and 3-D conditions were different, and radiation also induced AR according to morphological texture analysis. Priming low dose radiation induced AR both under monolayer and 3-D condition. Interestingly, 3-D microenvironment made cells more sensitive to radiation. The expression of p53 and p21 was changed and indicated that they might participate in the regulation of AR.

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Does single-dose cell resistance to the radio-mimetic zeocin correlate with a zeocin-induced adaptive response in Chlamydomonas reinhardtii strains?

Cited by 17 publications

References 35 publications

Extract of Lillium candidum L. Can Modulate the Genotoxicity of the Antibiotic Zeocin

Extract of Lillium candidum L. Can Modulate the Genotoxicity of the Antibiotic Zeocin

Liliumcompounds kaempferol and jatropham can modulate cytotoxic and genotoxic effects of radiomimetic zeocin in plants and human lymphocytesIn vitro

Ionizing Radiation-Induced Adaptive Response in Fibroblasts under Both Monolayer and 3-Dimensional Conditions

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