Dog factor differences in Can f 1 allergen production

Ramadour, M.; Guetat, M.; Guetat, J.; Biaze, M. El; Magnan, A.; Vervloët, D.

doi:10.1111/j.1398-9995.2005.00824.x

Cited by 31 publications

(33 citation statements)

References 15 publications

(14 reference statements)

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“…There may be several possible reasons for the observed heterogeneities of the extracts: (i) different starting material from which manufacturers extract allergens (hair, dander or/and epithelium) may contain different allergens [8,26], (ii) the levels of the major dog allergen Can f 1 may vary between dog breed and sex and between individual dogs of the same breed [27], (iii) different extraction techniques as well as the presence of stabilizers like glycerol and phenol might contribute to differences in extract composition [14,28]. …”

Section: Discussionmentioning

confidence: 99%

Skin Prick Test Extracts for Dog Allergy Diagnosis Show Considerable Variations Regarding the Content of Major and Minor Dog Allergens

Curin¹,

Reininger²,

Swoboda³

et al. 2010

Int Arch Allergy Immunol

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Background: Commercial skin prick test (SPT) extracts used for the diagnosis of dog allergy are prepared by extracting allergens from natural sources, e.g. dog hair and dander. Due to different starting material and extraction methods used, it is likely that extracts differ regarding their allergen contents. Methods: The total protein content and composition of dog SPT extracts from 5 European manufacturers were compared by silver-stained SDS-PAGE. Specific antibody probes were generated to detect major and minor allergens in each extract by immunoblotting. Additionally, sera of patients suffering from dog allergy were used to detect dog allergens in SPT extracts. Results: SPT extracts showed a 20-fold variation regarding the total protein content. The contents of the major dog allergen Can f 1 and of Can f 2 varied considerably between the extracts. In one of the extracts, neither Can f 1 nor Can f 2 could be detected by immunoblotting. The contents of the minor dog allergen Can f 3, albumin, also showed great variability. In one of the dog SPT extracts, the presence of human serum albumin (HSA) was detected with HSA-specific antibodies. Conclusion: The observed variability of commercial dog SPT extracts regarding their allergen contents likely has a negative influence on the accuracy of diagnosis of dog allergy.

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Section: Discussionmentioning

confidence: 99%

Skin Prick Test Extracts for Dog Allergy Diagnosis Show Considerable Variations Regarding the Content of Major and Minor Dog Allergens

Curin¹,

Reininger²,

Swoboda³

et al. 2010

Int Arch Allergy Immunol

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show abstract

“…4,6 Breed-specific allergens have not been identified. 4,6,7 Only 2 studies 6,8 have explored differences between levels of Can f 1, a major dog allergen (Canis familiaris), 4,9 in hair from different dog breeds.…”

mentioning

confidence: 99%

Can f 1 levels in hair and homes of different dog breeds: Lack of evidence to describe any dog breed as hypoallergenic

Vredegoor¹,

Willemse²,

Chapman³

et al. 2012

Journal of Allergy and Clinical Immunology

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“…However, this unequal distribution on fur could result from licking and grooming which is specific for cats. In contrast, Can f 1 concentrations have been found to be similar between different anatomical zones of dogs [16] . According to Kurosumi et al [25] , nonprimate mammals have apocrine sweat glands (producers of Bos d 2 in cattle) over the greater part of their body.…”

Section: Discussionmentioning

confidence: 81%

“…Ramadour et al [16] reported that male dogs had significantly higher Can f 1 concentrations in their hair compared to female dogs, although the difference was small (11.75 vs. 8.89 μg/g, p = 0.037) [16] . No significant effect of sex on Can f 1 levels was seen in the study by Vredegoor et al [15] .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Allergen Levels in the Hair of Different Cattle Breeds

Zahradnik¹,

Sander²,

Brüning³

et al. 2015

Int Arch Allergy Immunol

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Background: Cattle are well-known sources of respiratory allergens in agricultural environments. Breed-specific differences in Bos d 2 (a major bovine allergen) levels in cattle hair have been previously suggested but not fully characterized. Therefore, the aim of the current study was to determine whether hair from common cattle breeds differs in protein and allergen content. Methods: In total, 80 hair samples from 16 different cattle breeds were analyzed. The protein concentration was determined using the Bradford assay. The allergen content was measured using a sandwich ELISA based on polyclonal antibodies against a bovine hair protein extract and a commercial immunoassay based on monoclonal antibodies against Bos d 2. Results are given in micrograms per gram of hair. Statistical analysis was performed using the Kruskal-Wallis test and Spearman's rank correlation. Results: A wide variability in all 3 tested parameters was observed between the individual samples. The protein content differed by about 35-fold (0.3-12 mg/g), the bovine hair allergen content differed by about 500-fold (37-18,553 µg/g), and the Bos d 2 content differed by about 1,200-fold (5-6,323 µg/g). Protein, bovine hair allergen, and Bod d 2 values correlated strongly and significantly with one another. The median Bos d 2/bovine hair allergen ratio was 0.28. No significant differences were found between the most common breeds in Germany (Simmental, Holstein, and Braunvieh) and a group of rare breeds or between female and male animals. Conclusions: The results confirm a high variability in allergen levels between individual animals but also indicate that allergen production is related neither to the breed nor to gender.

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Dog factor differences in Can f 1 allergen production

Abstract: Breeds (Labrador retriever), sex and seborrhea seem to influence the levels of Can f 1 on fur.

Cited by 31 publications

References 15 publications

Skin Prick Test Extracts for Dog Allergy Diagnosis Show Considerable Variations Regarding the Content of Major and Minor Dog Allergens

Skin Prick Test Extracts for Dog Allergy Diagnosis Show Considerable Variations Regarding the Content of Major and Minor Dog Allergens

Can f 1 levels in hair and homes of different dog breeds: Lack of evidence to describe any dog breed as hypoallergenic

Allergen Levels in the Hair of Different Cattle Breeds

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