Dominance of Desulfotignum in sulfate-reducing community in high sulfate production-water of high temperature and corrosive petroleum reservoirs

Li, Xiaoxiao; Liu, Jinfeng; Yao, Feng; Wu, Wei-Lin; Yang, Shi‐Zhong; Mbadinga, Serge Maurice; Gu, Ji‐Dong; Mu, Bo‐Zhong

doi:10.1016/j.ibiod.2016.05.018

Cited by 50 publications

(21 citation statements)

References 76 publications

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“…At the class level, Deltaproteobacteria was the dominant class in the majority of corrosion samples. This observation parallels information from studies of the bacterial communities of samples collected from water-flooded petroleum reservoirs, water injection systems of Brazilian offshore oil platforms, and corrosive petroleum reservoirs in Yangzhou ( Korenblum et al, 2010 ; Li et al, 2016 ; Tian et al, 2017 ). There are many important SRB groups belonging to this class, e.g., Desulfovibrio , Desulfobacter and Desulfonatronum .…”

Section: Discussionsupporting

confidence: 73%

Analysis of Bacterial Community Composition of Corroded Steel Immersed in Sanya and Xiamen Seawaters in China via Method of Illumina MiSeq Sequencing

Duan

Xiao

et al. 2017

Front. Microbiol.

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Metal corrosion is of worldwide concern because it is the cause of major economic losses, and because it creates significant safety issues. The mechanism of the corrosion process, as influenced by bacteria, has been studied extensively. However, the bacterial communities that create the biofilms that form on metals are complicated, and have not been well studied. This is why we sought to analyze the composition of bacterial communities living on steel structures, together with the influence of ecological factors on these communities. The corrosion samples were collected from rust layers on steel plates that were immersed in seawater for two different periods at Sanya and Xiamen, China. We analyzed the bacterial communities on the samples by targeted 16S rRNA gene (V3–V4 region) sequencing using the Illumina MiSeq. Phylogenetic analysis revealed that the bacteria fell into 13 phylotypes (similarity level = 97%). Proteobacteria, Firmicutes and Bacteroidetes were the dominant phyla, accounting for 88.84% of the total. Deltaproteobacteria, Clostridia and Gammaproteobacteria were the dominant classes, and accounted for 70.90% of the total. Desulfovibrio spp., Desulfobacter spp. and Desulfotomaculum spp. were the dominant genera and accounted for 45.87% of the total. These genera are sulfate-reducing bacteria that are known to corrode steel. Bacterial diversity on the 6 months immersion samples was much higher than that of the samples that had been immersed for 8 years (P < 0.001, Student’s t-test). The average complexity of the biofilms from the 8-years immersion samples from Sanya was greater than those from Xiamen, but not significantly so (P > 0.05, Student’s t-test). Overall, the data showed that the rust layers on the steel plates carried many bacterial species. The bacterial community composition was influenced by the immersion time. The results of our study will be of benefit to the further studies of bacterial corrosion mechanisms and corrosion resistance.

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Section: Discussionsupporting

confidence: 73%

Analysis of Bacterial Community Composition of Corroded Steel Immersed in Sanya and Xiamen Seawaters in China via Method of Illumina MiSeq Sequencing

Duan

Xiao

et al. 2017

Front. Microbiol.

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“…For example, Desulfotignum , which was one of the shared dominant populations with its relative abundances in the different reservoirs ranging from 9.7 to 43.2%. Li et al (2016) also found Desulfotignum is the most dominant SRP in high temperature and corrosive petroleum reservoirs. For SOB assembly, differences were observed in the diversity and compositions among petroleum reservoirs ( Figure 7B ).…”

Section: Discussionmentioning

confidence: 83%

Compositions and Abundances of Sulfate-Reducing and Sulfur-Oxidizing Microorganisms in Water-Flooded Petroleum Reservoirs with Different Temperatures in China

et al. 2017

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Sulfate-reducing bacteria (SRB) have been studied extensively in the petroleum industry due to their role in corrosion, but very little is known about sulfur-oxidizing bacteria (SOB), which drive the oxidization of sulfur-compounds produced by the activity of SRB in petroleum reservoirs. Here, we surveyed the community structure, diversity and abundance of SRB and SOB simultaneously based on 16S rRNA, dsrB and soxB gene sequencing, and quantitative PCR analyses, respectively in petroleum reservoirs with different physicochemical properties. Similar to SRB, SOB were found widely inhabiting the analyzed reservoirs with high diversity and different structures. The dominant SRB belonged to the classes Deltaproteobacteria and Clostridia, and included the Desulfotignum, Desulfotomaculum, Desulfovibrio, Desulfobulbus, and Desulfomicrobium genera. The most frequently detected potential SOB were Sulfurimonas, Thiobacillus, Thioclava, Thiohalomonas and Dechloromonas, and belonged to Betaproteobacteria, Alphaproteobacteria, and Epsilonproteobacteria. Among them, Desulfovibrio, Desulfomicrobium, Thioclava, and Sulfurimonas were highly abundant in the low-temperature reservoirs, while Desulfotomaculum, Desulfotignum, Thiobacillus, and Dechloromonas were more often present in high-temperature reservoirs. The relative abundances of SRB and SOB varied and were present at higher proportions in the relatively high-temperature reservoirs. Canonical correspondence analysis also revealed that the SRB and SOB communities in reservoirs displayed high niche specificity and were closely related to reservoir temperature, pH of the formation brine, and sulfate concentration. In conclusion, this study extends our knowledge about the distribution of SRB and SOB communities in petroleum reservoirs.

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“…The dominant SRB in Nw/o and N28 were identified as Desulfotignum sp., suspected to be the main degrader of toluene under all conditions. These bacteria have been reported as potential threat to cause souring by reducing sulfate while using toluene and VFA as an electron donor as well as the main carbon source (Hasegawa et al 2014;Tanji et al 2014, Li et al 2016. By contrast, in N0 no VFA was detected from the beginning of the experiment, and the degradation of the hydrocarbon fraction was slow in comparison with the other conditions.…”

Section: Discussionmentioning

confidence: 99%

The presence of nitrate- and sulfate-reducing bacteria contributes to ineffectiveness souring control by nitrate injection

Kamarisima

Hidaka

Miyanaga

et al. 2018

International Biodeterioration & Biodegradation

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Nitrate injection has been widely used to minimize the production of biological hydrogen sulfide in oil and gas field industry. In environments exposed to seawater injection, nitrate is used to chemically and biologically control the growth of sulfate-reducing bacteria (SRB). The aim of this study was to investigate the changes in the bacterial community in response to nitrate addition used to control biological souring. Specifically, we investigated the effect of nitrate addition in an artificial souring experiment, using diluted crude oil as substrate and electron donor. Desulfotignum sp. was the predominant SRB under all conditions tested. Addition of nitrate at the beginning (N0) repressed the growth of SRB, as revealed by chemical and bacterial community analysis, concomitant with significant growth of the nitrate-reducing bacteria (NRB) Thalassospira sp. Nitrate addition after SRB growth (at day 28, N28) successfully remediated the sulfide produced by SRB, but no significant reduction in sulfate was observed subsequently; moreover, the bacterial communities before and after nitrate addition remained identical. The phenomenon in the N28 experiment might be the result of the role of Arcobacter sp., which are nitrate-reducing sulfide-oxidizing bacteria, and/or the ability of Desulfotignum sp. to 2 reduce nitrate and/or nitrite as a stress response. Thus, SRB might persist after nitrate addition, potentially causing subsequent SRB outbreaks.

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Dominance of Desulfotignum in sulfate-reducing community in high sulfate production-water of high temperature and corrosive petroleum reservoirs

Cited by 50 publications

References 76 publications

Analysis of Bacterial Community Composition of Corroded Steel Immersed in Sanya and Xiamen Seawaters in China via Method of Illumina MiSeq Sequencing

Analysis of Bacterial Community Composition of Corroded Steel Immersed in Sanya and Xiamen Seawaters in China via Method of Illumina MiSeq Sequencing

Compositions and Abundances of Sulfate-Reducing and Sulfur-Oxidizing Microorganisms in Water-Flooded Petroleum Reservoirs with Different Temperatures in China

The presence of nitrate- and sulfate-reducing bacteria contributes to ineffectiveness souring control by nitrate injection

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