Donghicola tyrosinivorans sp. nov., a tyrosine-degrading bacterium isolated from seawater

Abstract

A Gram-stain-negative, non-motile, aerobic bacterium, strain 13-93-B1 T , was isolated from seawater off Jeju Island, Republic of Korea, and was subjected to polyphasic taxonomic study. Cells formed ivory colonies and were ovoid to rod-shaped. The strain was catalase-positive, oxidase-negative and grew optimally at 30 8C, in the presence of 1-2 % (w/v) NaCl and at pH 7.0-7.5.It did not synthesize bacteriochlorophyll a. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain 13-93-B1 T clustered with the type strain Donghicola eburneus SW-277 T (97.0 % 16S rRNA gene sequence similarity). DNA-DNA hybridization between strain 13-93-B1 T and D. eburneus KCTC 12735 T was 33.1¡1.4 % (35.2¡2.8 % in a reciprocal experiment). The predominant cellular fatty acid was summed feature 8 (C 18 : 1 v7c/C 18 : 1 v6c; 76.9 %). The major respiratory quinone was ubiquinone Q-10 and polar lipids detected in strain 13-93-B1 T were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, an unidentified aminolipid and unidentified lipids. The DNA G+C content of strain 13-93-B1 T was 60.4 mol%. Based on the phenotypic, chemotaxonomic and phylogenetic data presented, strain 13-93-B1 T is considered to represent a novel species of the genus Donghicola, for which the name Donghicola tyrosinivorans sp. nov. is proposed. The type strain is 13-93-B1 T (5DSM 100212 T 5KCTC 42571 T ).The family Rhodobacteraceae , belonging to the order Rhodobacterales of the class Alphaproteobacteria (Garrity et al., 2005), encompasses a diverse group of genera. The Rhodobacteraceae have been established as one of the most abundant groups in diverse marine habitats Buchan et al., 2005) and, in recent times, many novel strains of the Rhodobacteraceae have been isolated from a variety of marine habitats. The genus Donghicola was created by Yoon et al. The screening of novel micro-organisms found in seawater collected off Jeju Island, Republic of Korea, has resulted in the identification of many novel micro-organisms. This study is focused on one of these isolates, designated 13-93-B1 T . Phylogenetic analysis based on the 16S rRNA gene sequence showed that this novel strain belongs to the genus Donghicola and forms a robust cluster with D. eburneus SW-277 T (97.0 % 16S rRNA gene sequence similarity). The aim of this study was to determine the exact taxonomic position of strain 13-93-B1 T by using a polyphasic approach that included morphological, physiological, biochemical and chemotaxonomic characterization, as well as phylogenetic analysis.Strain 13-93-B1 T was isolated from a surface seawater sample collected in September 2013, adjacent to Seogwipo-si, on Jeju Island. The strain was isolated on halfstrength marine agar 2216 (MA; BD) (Yang et al., 2006), by means of the standard dilution plating technique and incubation at 25 8C for 1 week. An ivory-coloured colony of strain 13-93-B1 T was separated and purified. The isolate was routinely cultivated on MA, and preserved at...

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