Donkey milk lysozyme ameliorates dextran sulfate sodium-induced colitis by improving intestinal barrier function and gut microbiota composition

Jiang, Libin; Lv, Jiayi; Li, Jingwen; Hao, Xianghui; Ren, Fazheng; Guo, Huiyuan

doi:10.1016/j.jff.2018.07.005

Cited by 30 publications

(27 citation statements)

References 63 publications

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“…Bifidobacterium, a beneficial microbial species, producing lactic acid and acetic acid, can reduce intestinal pH and inhibit the growth of various detrimental bacteria to maintain intestinal health (Wang R. et al, 2018). Bacteroides, Alistipes, Lachnospiraceae NK4A136 group and Alloprevotella are also capable of producing SCFAs such as acetic acid and butyric acid (Borton et al, 2017;Gotoh et al, 2017;Jiang et al, 2018;Yin et al, 2018). It was reported that the abundance of Bacteroides was reduced in individuals with atherosclerotic cardiovascular disease (Jie et al, 2017) and post-inflammatory irritable bowel syndrome .…”

Section: Discussionmentioning

confidence: 99%

“…It was reported that the abundance of Bacteroides was reduced in individuals with atherosclerotic cardiovascular disease (Jie et al, 2017) and post-inflammatory irritable bowel syndrome . Alistipes can effectively inhibit inflammation via preventing LPS-induced TNF-α release at higher concentrations (Canfora et al, 2015) and has been found at lower levels in the gut of patients with hepatocellular carcinoma (Ren et al, 2019), colitis (Jiang et al, 2018) and non-alcoholic fatty liver disease (Tang et al, 2018). Previous studies have indicated that Lachnospiraceae NK4A136 group may have an anti-inflammatory effect and its relative abundance was reduced in mice with immune dysfunction caused by cyclophosphamide .…”

Section: Discussionmentioning

confidence: 99%

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Probiotic Mixture of Lactobacillus plantarum Strains Improves Lipid Metabolism and Gut Microbiota Structure in High Fat Diet-Fed Mice

Liu

et al. 2020

Front. Microbiol.

123

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The global prevalence of obesity is rising year by year, which has become a public health problem worldwide. In recent years, animal studies and clinical studies have shown that some lactic acid bacteria possess an anti-obesity effect. In our previous study, mixed lactobacilli (Lactobacillus plantarum KLDS1.0344 and Lactobacillus plantarum KLDS1.0386) exhibited anti-obesity effects in vivo by significantly reducing body weight gain, Lee's index and body fat rate; however, its underlying mechanisms of action remain unclear. Therefore, the present study aims to explore the possible mechanisms for the inhibitory effect of mixed lactobacilli on obesity. C57BL/6J mice were randomly divided into three groups including control group (Control), high fat diet group (HFD) and mixed lactobacilli group (MX), and fed daily for eight consecutive weeks. The results showed that mixed lactobacilli supplementation significantly improved blood lipid levels and liver function, and alleviated liver oxidative stress. Moreover, the mixed lactobacilli supplementation significantly inhibited lipid accumulation in the liver and regulated lipid metabolism in epididymal fat pads. Notably, the mixed lactobacilli treatment modulated the gut microbiota, resulting in a significant increase in acetic acid and butyric acid. Additionally, Spearman's correlation analysis found that several specific genera were significantly correlated with obesity-related indicators. These results indicated that the mixed lactobacilli supplementation could manipulate the gut microbiota and its metabolites (acetic acid and butyric acid), resulting in reduced liver lipid accumulation and improved lipid metabolism of adipose tissue, which inhibited obesity.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Probiotic Mixture of Lactobacillus plantarum Strains Improves Lipid Metabolism and Gut Microbiota Structure in High Fat Diet-Fed Mice

Liu

et al. 2020

Front. Microbiol.

123

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“…Gut is the most important organs for absorption of nutrients in the whole body. 16 Notably, IBD seriously affect people's lives with increasing incidence and prevalence. 17 Recent therapies could ameliorate IBD, but direct therapeutic medicines do not yet exist.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of proprotein convertase subtilisin/kexin type 9 attenuates 2,4,6‐trinitrobenzenesulfonic acid‐induced colitis via repressing toll‐like receptor 4/nuclear factor‐kappa B

Lei

Yuan

et al. 2020

The Kaohsiung J of Med Scie

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Inflammatory bowel disease (IBD) is characterized by recurring inflammatory disorders in digestive system, and devoid of effective treatment. Proprotein convertase subtilisin/kexin type 9 (PCSK9), stimulated via inflammation whose inhibition could decrease secretion of inflammatory factors. We then determined whether inhibition of PCSK9 could improve the inflammation. First, rats model of colitis was first established via administration of 2,4,6‐trinitrobenzenesulfonic acid (TNBS), and then verified via determination of body weight loss, myeloperoxidase (MPO) activity, and histopathological analysis of colonic damage. Results showed that treatment with TNBS induced a great body weight loss, MPO activity increase, and serious colonic damage, showing an obviously character of IBD. PCSK9 was elevated in TNBS‐induced rats, and PCSK9 inhibition delivered by adenovirus vector increased the body weight, decreased MPO activity, and ameliorated histological change of colon. Second, the protective effect of PCSK9 inhibition against TNBS‐induced colitis was accompanied by decrease of proinflammatory factors secretion, including tumor necrosis factor‐α, interleukin‐1β, interleukin‐6, intercellular adhesion molecule 1, and monocyte chemoattractant protein‐1. TNBS could activate toll‐like receptor 4 (TLR4)/nuclear factor‐kappa B (NF‐κB) signaling pathway, while PCSK9 inhibition suppressed activation of TLR4/NF‐κB in TNBS‐induced rats. In conclusion, PCSK9 inhibition attenuated TNBS‐induced rat colitis through anti‐inflammatory effect under inactivation of TLR4/NF‐κB, suggesting potential therapeutic strategy in IBD.

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“…DM have shown immunological activities in vitro tests [ 42 , 45 ] and randomized controlled studies in animal models [ 46 ] and humans [ 47 ], in whom DM can induce the release of some cytokines, proteins that regulate the inflammatory and immune response to infections ( Table 3 and Table 4 ). DM has been shown to increase cytokines involved in the regulation of innate immunity and the onset of local acute inflammatory response: interleukin 1 (IL-1) [ 42 , 45 , 47 ], interleukin 6 (IL-6) [ 42 , 47 ] and tumor necrosis factor α (TNF-α) [ 42 , 47 ] both in vitro [ 42 ] and in vivo [ 47 ].…”

Section: Immunomodulatory Effectsmentioning

confidence: 99%

Current Knowledge on Functionality and Potential Therapeutic Uses of Donkey Milk

Martini

Altomonte

Tricò

et al. 2021

Animals

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The increase of knowledge on the composition of donkey milk has revealed marked similarities to human milk, which led to a growing number of investigations focused on testing the potential effects of donkey milk in vitro and in vivo. This paper examines the scientific evidence regarding the beneficial effects of donkey milk on human health. Most clinical studies report a tolerability of donkey milk in 82.6–98.5% of infants with cow milk protein allergies. The average protein content of donkey milk is about 18 g/L. Caseins, which are main allergenic components of milk, are less represented compared to cow milk (56% of the total protein in donkey vs. 80% in cow milk). Donkey milk is well accepted by children due to its high concentration of lactose (about 60 g/L). Immunomodulatory properties have been reported in one study in humans and in several animal models. Donkey milk also seems to modulate the intestinal microbiota, enhance antioxidant defense mechanisms and detoxifying enzymes activities, reduce hyperglycemia and normalize dyslipidemia. Donkey milk has lower calorie and fat content compared with other milks used in human nutrition (fat ranges from 0.20% to 1.7%) and a more favourable fatty acid profile, being low in saturated fatty acids (3.02 g/L) and high in alpha-linolenic acid (about 7.25 g/100 g of fat). Until now, the beneficial properties of donkey milk have been mostly related to whey proteins, among which β-lactoglobulin is the most represented (6.06 g/L), followed by α-lactalbumin (about 2 g/L) and lysozyme (1.07 g/L). So far, the health functionality of donkey milk has been tested almost exclusively on animal models. Furthermore, in vitro studies have described inhibitory action against bacteria, viruses, and fungi. From the literature review emerges the need for new randomized clinical trials on humans to provide stronger evidence of the potential beneficial health effects of donkey milk, which could lead to new applications as an adjuvant in the treatment of cardiometabolic diseases, malnutrition, and aging.

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Donkey milk lysozyme ameliorates dextran sulfate sodium-induced colitis by improving intestinal barrier function and gut microbiota composition

Cited by 30 publications

References 63 publications

Probiotic Mixture of Lactobacillus plantarum Strains Improves Lipid Metabolism and Gut Microbiota Structure in High Fat Diet-Fed Mice

Probiotic Mixture of Lactobacillus plantarum Strains Improves Lipid Metabolism and Gut Microbiota Structure in High Fat Diet-Fed Mice

Inhibition of proprotein convertase subtilisin/kexin type 9 attenuates 2,4,6‐trinitrobenzenesulfonic acid‐induced colitis via repressing toll‐like receptor 4/nuclear factor‐kappa B

Current Knowledge on Functionality and Potential Therapeutic Uses of Donkey Milk

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