Donnè Structurales et Ultrastructurales Nouvelles sur la Conjugaison de <i>Paramecium caudatum</i>.

Vivier, Emile

doi:10.1111/j.1550-7408.1961.tb01237.x

Cited by 37 publications

(8 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Inasmuch as formation of a cytoplasmic continuity between two conjugating cells through openings of 0.2-0 .5 p,m has been reported in P. caudatum (23), the same interpretation as Berger's can be given to the change of CNR phenotype to wild-type phenotype during conjugation . If the phenotypic change of the cnrC during conjugation is induced by cytoplasmic exchange between mates, microinjection of wild-type cytoplasm into cells of cnrC may be expected to produce change of the cnrC phenotype to wild-type.…”

Section: Microinjection Of Wild-type Cytoplasm Into Cnr Cellssupporting

confidence: 49%

“…Membrane-membrane interaction has been reported in fused cells between cell lines of mouse and human origin (8) in which specific surface antigens spread and intermix within minutes after membrane fusion . In Paramecium, membrane fusion in the region where cytoplasmic connnections between mates are formed has often been reported (20,23), and, in P. caudatum, this occurs immediately after the formation of tight pairs (paroral union) .' Thus, transfer of normal membrane sites through membrane fluidity cannot be ruled out .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Restoration of membrane excitability in a behavioral mutant of Paramecium caudatum during conjugation and by microinjection of wild-type cytoplasm.

Hiwatashi¹,

Haga²,

Takahashi³

1980

The Journal of Cell Biology

View full text Add to dashboard Cite

When cells of the behavioral mutant cnrC of Paramecium caudatum were mated with the wild type, phenotype change from CNR (no backward swinning) to wild type in the cnrC mate occurred immediately after the formation of tight pairs. No change of phenotype occurred when cells of cnrA or cnrB were mated with wild type. Phenotypic change from CNR to wild type in cells of cnrC was also induced by microinjection of wild-type cytoplasm. Microinjection of wild-type cytoplasm induced no change in cells of cnrA or cnrB. Phenotypic change in the cnrC mate during conjugation can be explained by cytoplasmic exchange during conjugation, though transfer of membrane sites for excitability through membrane fluidity cannot be ruled out.

show abstract

Section: Microinjection Of Wild-type Cytoplasm Into Cnr Cellssupporting

confidence: 49%

Section: Discussionmentioning

confidence: 99%

Restoration of membrane excitability in a behavioral mutant of Paramecium caudatum during conjugation and by microinjection of wild-type cytoplasm.

Hiwatashi¹,

Haga²,

Takahashi³

1980

The Journal of Cell Biology

View full text Add to dashboard Cite

show abstract

“…With increased time, a wave of resorption induced by overexpressed Nrk17p progressed to the more anterior region ( Figure 5). This wave is reminiscent of a naturally occurring wave of resorption that traverses the preoral region of Paramecium before conjugation (Vivier and Andre, 1961). Cilia are also selectively lost from the preoral region of mating Tetrahymena (Wolfe and Grimes, 1979;Wolfe, 1985;Kiersnowska and Kaczanowski, 1993).…”

Section: Discussionmentioning

confidence: 99%

Members of the NIMA-related Kinase Family Promote Disassembly of Cilia by Multiple Mechanisms

Włoga

Camba

Rogowski

et al. 2006

MBoC

105

103

View full text Add to dashboard Cite

The genome of Tetrahymena thermophila contains 39 loci encoding NIMA-related kinases (NRKs), an extraordinarily large number for a unicellular organism. Evolutionary analyses grouped these sequences into several subfamilies, some of which have orthologues in animals, whereas others are protist specific. When overproduced, NRKs of three subfamilies caused rapid shortening of cilia. Ultrastructural studies revealed that each NRK triggered ciliary resorption by a distinct mechanism that involved preferential depolymerization of a subset of axonemal microtubules, at either the distal or proximal end. Overexpression of a kinase-inactive variant caused lengthening of cilia, indicating that constitutive NRK-mediated resorption regulates the length of cilia. Each NRK preferentially resorbed a distinct subset of cilia, depending on the location along the anteroposterior axis. We also show that normal Tetrahymena cells maintain unequal length cilia. We propose that ciliates used a large number of NRK paralogues to differentially regulate the length of specific subsets of cilia in the same cell. INTRODUCTIONHow the size of organelles is determined is an important and largely unanswered question. Cilia have emerged as a model organelle to study the mechanism of size regulation (Marshall, 2002). The size of an organelle is determined by the balance between assembly pathways that deliver structural components and disassembly pathways that remove components. In cilia, the intraflagellar transport (IFT), a bidirectional motility system that operates inside cilia (Rosenbaum and Witman, 2002;Scholey, 2003), plays a key role in length regulation. The anterograde IFT supplies structural subunits during assembly (Piperno et al., 1996;Qin et al., 2005) and maintains the axoneme after assembly (Brown et al., 1999). The retrograde IFT recycles the IFT machinery (Signor et al., 1999) and removes ciliary structural components that turn over (Qin et al., 2004). This turnover is the basis of the disassembly pathway (Stephens, 1997;Marshall and Rosenbaum, 2001;Song and Dentler, 2001;Thazhath et al., 2004). Experimental data and mathematical modeling in Chlamydomonas reinhardtii indicate that the length of flagella is dependent on the rates of elongation (equivalent to the efficiency of anterograde IFT) and disassembly. As the flagellum grows, the rate of elongation decreases due to the limited supply of IFT components that need to travel longer distances to the tip (Marshall and Rosenbaum, 2001). According to the "balance point model," the steady-state length is achieved when the rate of elongation equals the rate of disassembly (Marshall and Rosenbaum, 2001;Marshall et al., 2005).The length of already assembled cilia can be reduced drastically using two mechanisms: autotomy or resorption (reviewed by Quarmby, 2004). During autotomy, the axoneme breaks off from the basal body, the cilium falls off, and the plasma membrane reseals over the basal body. Protists undergo autotomy in response to low pH (Lewin et al., 1982). It is unclear whether...

show abstract

“…The fact that intracellular structures as large as micronuclei and Chlorella are transferred from cell to cell suggests that conjugants are connected by cytoplasmic bridges. Indeed, electronmicroscopical investigations have demonstrated that they exist in T. pyriformis (ELLIOT and TREMOR, 1958), P. aurelia (JURAND and SELMAN, 1969), P. caudatum (VIVIER and ANDRE, 1961); VIVIER, 1962), P. multimicronucleatum (INABA et aI., 1966), E. vanus (NOBILl, 1967). Many of these cytoplasmic bridges are less than 1 {1. in width but a bridge as wide as 10 microns was also observed in P. aurelia (SCHNEIDER, 1963) and B. intermedium (OTOTAKE, 1969).…”

Section: A Genetic Interactionmentioning

confidence: 96%

Current Topics in Microbiology and Immunology

1974

Current Topics in Microbiology and Immunology

View full text Add to dashboard Cite

Published by Springer-Verlag Price : 129.95 € Hardcover Pages : 255, Vol 340, 2010 ISBN : 978-3-642-03857-0 DOI : 10.1007 Reviewed by Rajendra Kumar and Rupali Sharma "Current topics in microbiology and immunology: Immunological Synapse" Reviewed by Rajendra Kumar and Rupali Sharma.Published by Springer pp 255, Price 129.95 €(Hardcover).The science of immunology has seen outsized transformation from a medicine named vaccination to a contemporary applied science focused on versatile development in molecular medicine. Fundamental principles of immunology along with their clinical applications provide an exciting platform for specialization. Immune system is able to engender variety of cells and molecules capable of recognizing and removing infinite number of foreign intruders. Functioning of immune system is dependent on a reliable cell-cell communication, which forms the basis of phenomena of Immunological Synapse.Current volume is an attempt of an additional step toward understanding the immunological synapse. It has the essence of most consensual volume, as editors invited a number of key researchers in the field, to provide perspectives and current understanding of the molecular process in the wake of the formation and function of the immunological synapse. This book targets the professionals as well as the medical or bioscience students by emphasizing on the recent advancement in immunological synapse.In first chapter, Michael L. Dustin has enlighten the function of immunological synapse and have explained the basic features of molecular interactions in the synapse using the lipid bi-layer on glass surface model. Further he has also explained that how this model along with fluorescent microscopy, can be exploited for the measurement of 2-D affinity and kinetics in cell-cell contact.Junsang Doh and Matthew F. Krummel have contributed the second chapter. They have reviewed the pattern of cell-cell communication in T lymphocytes and antigen presenting cells and extrapolated it into the framework of immune system as a whole. Here, the authors have suggested various patterns of cell-cell communication in the immune system and have described that how each type of pattern suffice the need of an efficient communication in the system. Yolanda R. Carrasco has presented his perspective on B-cell synapse by spreading-contraction response theory of B cell activation in the succeeding chapter. Here, authors have suggested that immunological synapse is crucial for increasing the sensibility of B cells to antigen. The authors have discussed the role of single cell imaging techniques like real-time confocal microscopy and total interference reflection fluorescence microscopy in the study of the molecular mechanisms beneath B cell activation.Since it is crucial to control the immune response for maintaining the self-tolerance, the inhibitory immune synapse comes into the picture. This view has been presented by Philipp Eissmann and Daniel M. Davis. Here, the authors have discussed the role of supra-molecular assemblies to co...

show abstract

Donnè Structurales et Ultrastructurales Nouvelles sur la Conjugaison de Paramecium caudatum.

Cited by 37 publications

References 9 publications

Restoration of membrane excitability in a behavioral mutant of Paramecium caudatum during conjugation and by microinjection of wild-type cytoplasm.

Restoration of membrane excitability in a behavioral mutant of Paramecium caudatum during conjugation and by microinjection of wild-type cytoplasm.

Members of the NIMA-related Kinase Family Promote Disassembly of Cilia by Multiple Mechanisms

Current Topics in Microbiology and Immunology

Contact Info

Product

Resources

About