Donor age and gender effects on osteoinductivity of demineralized bone matrix

Abstract

Allogeneic demineralized bone matrix (DBM) has been used extensively as a clinical graft material because of its inherent osteoinductive and osteoconductive properties. There is continued debate over the acceptable age range of donors for bone and whether the effectiveness of the tissue as a graft is influenced by gender. Contradictory evidence has been obtained with DBM prepared from both animals and humans. The goal of the present investigation was to evaluate the effect of donor age and gender on the osteoinductivity of DBM prepared from human donors [male (133) and female (115) donors grouped in 10-year age brackets up to 85 years] with a statistically relevant sample size using the athymic rat ectopic bone formation model. Among males, there was a statistically significant linear association between age and osteoinductivity value (p <.001), but not among females (p =.20). The rate of change among males was 0.009 units per year. The biological relevance of such a small change in osteoinductivity is likely to be negligible, as the total variation explained by the regressions was only 8.2%. A two-way ANOVA as related to donor age (only donors < 76 years of age) and gender yielded no significant statistical association of osteoinductivity with age group, gender, and their interaction. The results confirm that properly processed demineralized bone from donors through at least 85 years of age is a viable grafting material.

Osteoinductivity of Demineralized Bone Matrix Is Independent of Donor Bisphosphonate Use

“…No young donors were used in the present study; therefore, it is not known if there would have been a higher percent of the samples with osteoinductive properties in demineralized bone matrix processed from a young donor population. Our results support previous observations from our group 34 and others [52][53][54] , showing that donors who are sixty to seventy years of age continue to possess osteoinductive bone, and suggest that any loss of osteoinductivity occurs later in life.…”

Tissue engineering of bone: the reconstructive surgeon's point of view

“…It enhanced bone formation in experimental and clinical settings. Demineralized bone matrix (DBM) is prepared from allogenic or xenogenic bone and commercially available for clinical application in different formulations [46][47][48][49]. Its osteoinductive potential is highly variable and depends not only on the donor but also on the processing protocols.…”

Influence of hydrochloric acid concentration on the demineralization of cortical bone

“…In fact, different demineralization protocols have been adopted (Nade and Burwell, 1977;Walsh and Christiansen, 1995;Zhang et al, 1997), using not only different acids (and acid concentrations) but also different grafting materials (cortical or cancelous bone) under distinct configurations (from long bone to blocks and particles with different shape and size characteristics). In addition, donor specifications (Lohmann et al, 2001;Traianedes et al, 2004) and preservation methods (freeze-dried or fresh frozen) (Martin et al, 1999;Schwartz et al, 1996) have also proven to influence the properties of DBM. Furthermore, correlations with morpho-structural changes caused by demineralization are seldom described in the literature.…”