Donor-derived DNA in hair follicles of recipients after allogeneic hematopoietic stem cell transplantation

Jacewicz, Renata; Lewandowski, Krzysztof; Rupa‐Matysek, Joanna; Jędrzejczyk, Maciej; Brzeziński, Piotr; Dobosz, Tadeusz; Jonkisz, Anna; Szram, Stefan; Komarnicki, Mieczysław; Berent, Jarosław

doi:10.1038/bmt.2010.27

Cited by 16 publications

(9 citation statements)

References 29 publications

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“…The presented research, similar to the previously performed investigations [22], proved the aforementioned conviction to be false. The key to invalidating this belief was the application of highly sensitive and male-specific methods enabling the selective amplification of Y-chromosome markers.…”

Section: Resultssupporting

confidence: 87%

Genetic investigation of biological materials from patients after stem cell transplantation based on autosomal as well as Y-chromosomal markers

et al. 2012

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The authors presented the results of DNA polymorphism investigation of blood, buccal swabs and hair follicles originating from patients after allogeneic hematopoietic stem cell transplantation. The real-time and multiplex assays based on polymerase chain reaction within the range of autosomal as well as Y-chromosomal markers were applied to assess the possible dangers arising from investigation of these materials in forensic genetics. The results revealed that not only post-transplant blood and buccal swab, but also recipient hair, up to now regarded as devoid of any donor’s cells, do not constitute entirely safe material for forensic purposes. Their analysis can lead to the false identification of gender or male haplotype. The investigation of sex-determining region Y and Y-chromosome short tandem repeats performed in female recipients with male donors resulted in the designation of donor’s DNA in hair cells as well as in blood and buccal swabs. Therefore, biological stains gathered from crime scenes should not be analysed exclusively based on the investigation of male-specific markers.Electronic supplementary materialThe online version of this article (doi:10.1007/s00414-012-0771-x) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 87%

Genetic investigation of biological materials from patients after stem cell transplantation based on autosomal as well as Y-chromosomal markers

et al. 2012

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“…Extracted DNA from hair samples were amplified using Ampflstr Yfiler PCR amplification kit. They observed complete donor chimerism in hair samples of recipients [12]. Similar type of results were again reported by Jacewicz et al [13], with the increase in the number of samples analyzed (32 patients) and donor chimerism was observed in hair samples of recipients of Allo-HSCT.…”

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confidence: 76%

Chimerism in Humans after Bone Marrow Transplantation: A New Challenge for Forensic DNA Experts

Chaudhary¹

2016

J For Med Leg Aff

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“…The profiles indicated a mixture of the recipient and donor patterns in the buccal swab, the full donor pattern in the blood sample, and the full recipient profile in the hair follicle and sperm samples. However, previous studies revealed the Y-STR haplotype from the donor in all the post-transplant blood and buccal swab samples, and even in post-transplant hair follicle samples obtained from female patients following gender-mismatched engraftment (11,13). A previous study has already demonstrated that an extremely small quantity of male material in mixed male/female DNA samples can be detected by analysis with the Y-STR system (18).…”

Section: Resultsmentioning

confidence: 94%

DNA profiling in peripheral blood, buccal swabs, hair follicles and semen from a patient following allogeneic hematopoietic stem cells transplantation

Xie

Jin

2014

biom rep

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Allogeneic peripheral blood stem cells transplantation (allo-PBSCT) or allogeneic bone marrow transplantation (allo-BMT) have been widely used to treat patients exhibiting certain severe illnesses. However, previous studies have shown that the biological materials of allo-PBSCT or allo-BMT recipients may not constitute credible materials for personal identification. In the present study, four types of commonly used samples were collected from a male individual following gender-matched allo-BMT. Autosomal short tandem repeat (STR) and Y-STR markers analysis, based on polymerase chain reaction, were used to evaluate the chimerism status. The results showed that the blood sample were all donor type, the buccal swab sample were mixed chimerism, and the sperm and hair follicle samples maintained a recipient origin of 100%. In conclusion, identical results were obtained by the two methods and it was confirmed that DNA extracted from hair follicles and sperm can be used as a reference for the pre-transplant genotype DNA profile of the recipient in the gender-match allo-BMT or -PBSCT.

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Donor-derived DNA in hair follicles of recipients after allogeneic hematopoietic stem cell transplantation

Cited by 16 publications

References 29 publications

Genetic investigation of biological materials from patients after stem cell transplantation based on autosomal as well as Y-chromosomal markers

Genetic investigation of biological materials from patients after stem cell transplantation based on autosomal as well as Y-chromosomal markers

Chimerism in Humans after Bone Marrow Transplantation: A New Challenge for Forensic DNA Experts

DNA profiling in peripheral blood, buccal swabs, hair follicles and semen from a patient following allogeneic hematopoietic stem cells transplantation

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