Dopamine‐like immunoreactivity in the brain and suboesophageal ganglion of the honeybee

Schäfer, Sabine; Rehder, Vincent

doi:10.1002/cne.902800105

Cited by 128 publications

(142 citation statements)

References 39 publications

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“…We chose to examine dopamine-evoked responses in mushroom body calyces because these structures can be easily isolated from the brain. Moreover, intrinsic mushroom body neurons (Kenyon cells) express all three of the dopamine receptor genes identified in honey bees, Amdop1, Amdop2, and Amdop3 (25,26,29,30), and mushroom bodies receive extensive input from dopamine-immunoreactive neurons (31).…”

Section: Resultsmentioning

confidence: 99%

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Queen pheromone modulates brain dopamine function in worker honey bees

Beggs

Glendining²,

Marechal³

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

155

139

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Honey bee queens produce a sophisticated array of chemical signals (pheromones) that influence both the behavior and physiology of their nest mates. Most striking are the effects of queen mandibular pheromone (QMP), a chemical blend that induces young workers to feed and groom the queen and primes bees to perform colony-related tasks. But how does this pheromone operate at the cellular level? This study reveals that QMP has profound effects on dopamine pathways in the brain, pathways that play a central role in behavioral regulation and motor control. In young worker bees, dopamine levels, levels of dopamine receptor gene expression, and cellular responses to this amine are all affected by QMP. We identify homovanillyl alcohol as a key contributor to these effects and provide evidence linking QMPinduced changes in the brain to changes at a behavioral level. This study offers exciting insights into the mechanisms through which QMP operates and a deeper understanding of the queen's ability to regulate the behavior of her offspring.Apis mellifera ͉ biogenic amines ͉ neuroethology ͉ neuromodulation ͉ pheromonal communication C omplex social interactions require systems of communication that are reliable and unambiguous. The honey bee, Apis mellifera, employs Ͼ50 substances to communicate with and to organize its colony, and the information that is conveyed between colony members is both subtle and sophisticated (1-3).Maintaining colony organization is a primary role of the queen, whose pheromones enable her to regulate not only the behavior but also the physiology of her nest mates. The most striking effects are those of queen mandibular pheromone (QMP), a chemical blend that induces young workers to feed and groom the queen (Fig. 1) and primes bees to perform colonyrelated tasks (4, 5). The retinue of workers that attend the queen facilitates the distribution of QMP throughout the colony, where it inhibits the rearing of new queens (6), helps prevent the development of worker ovaries (7), influences comb-building activities, (8) and affects the biosynthesis of juvenile hormone (9) [and hence the age-related behavioral ontogeny of recipient workers (10)]. Despite QMP's central role in the normal functioning and organization of honey bee colonies, very little is known about the cellular mechanisms through which it operates.We were intrigued by one of the key components of QMP, homovanillyl alcohol (HVA) (4). HVA (4-hydroxy-3-methoxyphenylethanol) bears a striking structural resemblance to dopamine (see Fig. 1 Right), a biogenic amine that plays a central role in insect behavioral regulation and motor control (11-18). The presence of this compound within the pheromone blend suggested to us that dopamine function in the brain of recipient bees might be affected by exposure to QMP. To test this hypothesis, we exposed young workers to QMP and examined its effects on brain dopamine levels, levels of dopamine receptor gene expression, and cellular responses to this amine. The results provide compelling evidence that QMP al...

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Section: Resultsmentioning

confidence: 99%

“…and A.R.M., unpublished data). Whether changes at the level of the mushroom bodies contribute to the QMP-induced changes in locomotor activity, however, remains unclear because many regions of the brain are densely innervated by dopaminergic neurons (31) and all are potential targets for modulation by QMP.…”

Section: Discussionmentioning

confidence: 99%

Queen pheromone modulates brain dopamine function in worker honey bees

Beggs

Glendining²,

Marechal³

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

155

139

View full text Add to dashboard Cite

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“…In Schäfer and Rehder's study, dopamine-like immunoreactive neurons were identified in most parts of the brain and in the suboesophageal ganglion (Schäfer and Rehder, 1989) (Fig.4). Only the optic lobes were devoid of staining.…”

Section: The Neural Basis Of Aversive Learning: Us Signalingmentioning

confidence: 86%

Rules and mechanisms of punishment learning in honey bees: the aversive conditioning of the sting extension response

Tedjakumala

Giurfa

2013

Journal of Experimental Biology

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SummaryHoneybees constitute established model organisms for the study of appetitive learning and memory. In recent years, the establishment of the technique of olfactory conditioning of the sting extension response (SER) has yielded new insights into the rules and mechanisms of aversive learning in insects. In olfactory SER conditioning, a harnessed bee learns to associate an olfactory stimulus as the conditioned stimulus with the noxious stimulation of an electric shock as the unconditioned stimulus. Here, we review the multiple aspects of honeybee aversive learning that have been uncovered using Pavlovian conditioning of the SER. From its behavioral principles and sensory variants to its cellular bases and implications for understanding social organization, we present the latest advancements in the study of punishment learning in bees and discuss its perspectives in order to define future research avenues and necessary improvements. The studies presented here underline the importance of studying honeybee learning not only from an appetitive but also from an aversive perspective, in order to uncover behavioral and cellular mechanisms of individual and social plasticity.

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“…Similarly, agonists could be designed that couple the DopR99B receptor to one or another of the two second messenger pathways that it potentially can activate. Such compounds could lead to the development of highly effective insect control agents, given the preferential expression of this Drosophila dopamine receptor in mushroom bodies (Han et al, 1996) and the likely involvement of dopamine in the processes of learning and memory in the insect nervous system (Tempel et al, 1984;Budnik and White, 1988;Schafer and Rehder, 1989;Buchner, 1991;Nassel and Elekes, 1992). Further, genetic studies on dopamine receptor mutants in Drosophila could identify the physiological roles of the separate activation of each of the two second messenger systems potentially coupled to the DopR99B receptor, because a variation in the local G-protein environment of different cell types expressing this receptor might allow the receptor to be coupled differently in different cell types.…”

Section: Discussionmentioning

confidence: 99%

Agonist-Specific Coupling of a ClonedDrosophila melanogasterD1-Like Dopamine Receptor to Multiple Second Messenger Pathways by Synthetic Agonists

et al. 1997

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The mechanism of coupling of a cloned Drosophila D1-like dopamine receptor, DopR99B, to multiple second messenger systems when expressed in Xenopus oocytes is described. The receptor is coupled directly to the generation of a rapid, transient intracellular Ca 2ϩ signal, monitored as changes in inward current mediated by the oocyte endogenous Ca 2ϩ -activated chloride channel, by a pertussis toxin-insensitive G-proteincoupled pathway. The more prolonged receptor-mediated changes in adenylyl cyclase activity are generated by an independent G-protein-coupled pathway that is pertussis toxinsensitive but calcium-independent, and G ␤␥ -subunits appear to be involved in the transduction of this response. This is the first evidence for the direct coupling of a cloned D1-like dopamine receptor both to the activation of adenylyl cyclase and to the initiation of an intracellular Ca 2ϩ signal. The pharmacological profile of both second messenger effects is identical for a range of naturally occurring catecholamine ligands (dopamine Ͼ norepinephrine Ͼ epinephrine) and for the blockade of dopamine responses by a range of synthetic antagonists. However, the pharmacological profiles of the two second messenger responses differ for a range of synthetic agonists. Thus, the receptor exhibits agonist-specific coupling to second messenger systems for synthetic agonists. This feature could provide a useful tool in the genetic analysis of the roles of the multiple second messenger pathways activated by this receptor, given the likely involvement of dopamine in the processes of learning and memory in the insect nervous system.

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Dopamine‐like immunoreactivity in the brain and suboesophageal ganglion of the honeybee

Cited by 128 publications

References 39 publications

Queen pheromone modulates brain dopamine function in worker honey bees

Queen pheromone modulates brain dopamine function in worker honey bees

Rules and mechanisms of punishment learning in honey bees: the aversive conditioning of the sting extension response

Agonist-Specific Coupling of a ClonedDrosophila melanogasterD1-Like Dopamine Receptor to Multiple Second Messenger Pathways by Synthetic Agonists

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