Dopamine secretion by PC12 cells microencapsulated in a hydroxyethyl methacrylate-methyl methacrylate copolymer

Roberts, T.; Boni, U. De; Sefton, Michael V.

doi:10.1016/0142-9612(96)85564-5

Cited by 54 publications

(22 citation statements)

References 35 publications

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“…Encapsulated PC12 growth was slower than growth in conventional two-dimensional (2-D) culture, as was noted previously. 22 The effect of capsule formation on PC12 growth was small; the effect for other cell types was more significant. In other studies, after 4 weeks in culture, L929 cells (a mouse fibroblast line) grew inside 9% HEMA-MMA/TEG plus PVP capsules to have an increase in MTT signal (from day 0) that was five times larger than the increase obtained for 10%/TEG capsules.…”

Section: Effect Of Capsule Formulation Previous Experimentsmentioning

confidence: 97%

Viability of Hydroxyethyl Methacrylate–Methyl Methacrylate-Microencapsulated PC12 Cells after Omental Pouch Implantation within Agarose Gels

Fleming

Sefton²

2003

Tissue Engineering

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Hydroxyethyl methacrylate-methyl methacrylate (HEMA-MMA, 75 mol% HEMA). Microcapsules containing viable PC12 cells (as an allogeneic transplant model) were implanted into omental pouches in Wistar rats. Two different capsule preparations were tested, based on differences in polymer solutions during extrusion: 10% HEMA-MMA in TEG, and 9% HEMA-MMA in TEG with 30% poly(vinyl pyrrolidone) (PVP). The omental pouch proved to be an ideal transplant site in terms of implantation, recovery, and blood vessel proximity (nutrient supply). To minimize the fibrous overgrowth and damaged capsules previously seen on implantation of individual capsules, agarose gels were used to embed the capsules before implantation. Cells proliferated within the microcapsule-agarose device during the first 7 days of implantation, but overall cell viability declined over the 3-week period, when compared with similar capsules maintained in vitro. Nonetheless, approximately 50% of the initial encapsulated cells were still viable after 3 weeks in vivo. This approach to HEMA-MMA microcapsule implantation improved cell viability and capsule integrity after 3 weeks in vivo, compared with capsules implanted without agarose.

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Section: Effect Of Capsule Formulation Previous Experimentsmentioning

confidence: 97%

Viability of Hydroxyethyl Methacrylate–Methyl Methacrylate-Microencapsulated PC12 Cells after Omental Pouch Implantation within Agarose Gels

Fleming

Sefton²

2003

Tissue Engineering

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“…Hypoxia is typically defined as prolonged exposure to 1% Oxygen (pO 2 = 7.6 mmHg). Since the idea of ''central necrosis'' in spheroidal culture and microencapsulated cells 85 has a long history in the tissue engineering literature, some studies have detailed the oxygen gradients through an engineered tissue in vitro. For example, Malda et al measured oxygen levels as low as 2-5% in the centre of in vitro cultured engineered cartilage using glass microelectrodes.…”

Section: Hypoxia Is Induced On Implantation Of Engineered Tissuementioning

confidence: 99%

In Vivo Remodelling of Vascularizing Engineered Tissues

et al. 2014

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A critical aspect of creating vascularized tissues is the remodelling that occurs in vivo, driven in large part by the host response to the tissue construct. Rather than a simple inflammatory response, a beneficial tissue remodelling response results in the formation of vascularised tissue. The characteristics and dynamics of this response are slowly being elucidated, especially as they are modulated by the complex interaction between the biomaterial and cellular components of the tissue constructs and the host. This process has elements that are similar to both wound healing and tumour development, and its features are illustrated by reference to the bottom-up generation of a tissue using modular constructs. These modular constructs consist of mesenchymal stromal cells (MSC) embedded in endothelial cell (EC)-covered collagen gel rods that are a few hundred microns in size. Particular attention is paid to the role of hypoxia and macrophage recruitment, as well as the paracrine effects of the MSC and EC in this host response.

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“…Cultivation governs several mammalian cells like parathyroid cells [63], hepatocytes [15,80], neurons [69,81], islets of langerhan's [12,58] and T-cells, as well as transfected hybridoma [10] cells. As a speciality, even infected insect cells have been grown under these conditions [29].…”

Section: Microencapsulationmentioning

confidence: 99%

Single-cell-bioreactors as end of miniaturization approaches in biotechnology: progresses with characterised bioreactors and a glance into the future

Meier

Huebner

2005

Bioprocess Biosyst Eng

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Incidents with single cells and their genesis have not been the major focus of science up to now. This fact is supported by the difficulties one faces when wanting to monitor and cultivate small populations of cells in a defined compartment under controlled conditions, in vitro. Several approaches of up- and down-scaling have often led to poorly understood results which might be better elucidated by understanding the cellular genesis as a function of its microenvironment. This review of the approaches of scale-up and scale-down processes illustrates technical possibilities and shows up their limitations with regard to obtainable data for the characterisation of cellular genesis and impact of the cellular microenvironment. For example, stem cell research advances underline the lack of information about the impact of the microenvironment on cellular development. Finally, a proposal of future research efforts is given on how to overcome this lack of data via a novel bioreactor setup.

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Dopamine secretion by PC12 cells microencapsulated in a hydroxyethyl methacrylate-methyl methacrylate copolymer

Cited by 54 publications

References 35 publications

Viability of Hydroxyethyl Methacrylate–Methyl Methacrylate-Microencapsulated PC12 Cells after Omental Pouch Implantation within Agarose Gels

Viability of Hydroxyethyl Methacrylate–Methyl Methacrylate-Microencapsulated PC12 Cells after Omental Pouch Implantation within Agarose Gels

In Vivo Remodelling of Vascularizing Engineered Tissues

Single-cell-bioreactors as end of miniaturization approaches in biotechnology: progresses with characterised bioreactors and a glance into the future

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