Dopamine, vesicular transporters and dopamine receptor expression and localization in rat thymus and spleen

Mignini, Fiorenzo; Tomassoni, Daniele; Traini, Enea; Amenta, Francesco

doi:10.1016/j.jneuroim.2008.09.018

Cited by 43 publications

(52 citation statements)

References 45 publications

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“…Intracellular storage of catecholamines in human lymphocytes occurs in reserpinesensitive compartments Cosentino et al 2000Cosentino et al , 2007, which suggests the involvement of vesicular monoamine transporters (VMAT) similar to those expressed in neural and neuroendocrine cells (Henry et al 1994), in agreement with preliminary evidence suggesting the occurrence of VMAT-1 and 2 in rat thymus and spleen (Mignini et al 2009) and possibly also in human peripheral blood lymphocytes (Amenta et al 2001) In particular, in human activated lymphocytes release can be effectively induced by biological agents such as interferon-b (Cosentino et al 2005) ] e may thus both assist the recruitment of lymphocytes to an injured tissue and lead to local increase of catecholamines, which in turn may act upon lymphocytes themselves and/or upon neighboring cells. It should be noted that, at least in vitro, catecholamine release from activated lymphocytes increases extracellular catecholamines from pico-nanomolar up to submicromolar concentrations (and possibly to even higher values in the vicinity of the cells), which may be well within the effective concentration range to exert immunomodulating effects.…”

Section: Storage and Releasesupporting

confidence: 70%

Nerve Driven Immunity: Noradrenaline and Adrenaline

Cosentino

Marino

2012

Nerve-Driven Immunity

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Section: Storage and Releasesupporting

confidence: 70%

Nerve Driven Immunity: Noradrenaline and Adrenaline

Cosentino

Marino

2012

Nerve-Driven Immunity

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“…Cytosolic DA can also be taken up from the extracellular environment through plasma-membrane dopamine transporters (DATs) (16). Once inside the cell, DA can either be inactivated by monoamine oxidases (MAO-A and -B) (17) or can be stored in intracellular vesicles. Storage is mediated by type 1 and type 2 vesicular monoamine transporters (VMATs 1 and 2) that mobilize cytosolic DA toward vesicular stores (18).…”

Section: Endritic Cells (Dcs) Are the Most Efficient Type Of Apcsmentioning

confidence: 99%

Stimulation of Dopamine Receptor D5 Expressed on Dendritic Cells Potentiates Th17-Mediated Immunity

Prado

Contreras

González

et al. 2012

The Journal of Immunology

116

132

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Dendritic cells (DCs) are responsible for priming T cells and for promoting their differentiation from naive T cells into appropriate effector cells. Emerging evidence suggests that neurotransmitters can modulate T cell-mediated immunity. However, the involvement of specific neurotransmitters or receptors remains poorly understood. In this study, we analyzed the role of dopamine in the regulation of DC function. We found that DCs express dopamine receptors as well as the machinery necessary to synthesize, store, and degrade dopamine. Notably, the expression of D5R decreased upon LPS-induced DC maturation. Deficiency of D5R on the surface of DCs impaired LPS-induced IL-23 and IL-12 production and consequently attenuated the activation and proliferation of Ag-specific CD4+ T cells. To determine the relevance of D5R expressed on DCs in vivo, we studied the role of this receptor in the modulation of a CD4+ T cell-driven autoimmunity model. Importantly, D5R-deficient DCs prophylactically transferred into wild-type recipients were able to reduce the severity of experimental autoimmune encephalomyelitis. Furthermore, mice transferred with D5R-deficient DCs displayed a significant reduction in the percentage of Th17 cells infiltrating the CNS without differences in the percentage of Th1 cells compared with animals transferred with wild-type DCs. Our findings demonstrate that by contributing to CD4+ T cell activation and differentiation to Th17 phenotype, D5R expressed on DCs is able to modulate the development of an autoimmune response in vivo.

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“…Samples (0.1 Ϯ 0.02 g) were lysed in a Mixer Mill MM300 (Qiagen, Hilden, Germany) with 800 l of lysing buffer, as previously described (22). Equal amounts of protein (40 g) were separated by 8% SDS-PAGE and transferred to nitrocellulose membrane (22).…”

Section: Western Blot Analysismentioning

confidence: 99%

“…Equal amounts of protein (40 g) were separated by 8% SDS-PAGE and transferred to nitrocellulose membrane (22). Transblotted membranes were incubated with DAT, VMAT-1, and VMAT-2 antibodies and dopamine D1, D2, D3, D4, and D5 receptor antibodies diluted 1:500.…”

Section: Western Blot Analysismentioning

confidence: 99%

“…Transblotted membranes were incubated with DAT, VMAT-1, and VMAT-2 antibodies and dopamine D1, D2, D3, D4, and D5 receptor antibodies diluted 1:500. The product of immune reaction was visualized by a chemiluminescence detection followed by computerdriven densitometry with an IAS 2000 image analyzer (Delta Sistemi, Rome, Italy), as previously described (22).…”

Section: Western Blot Analysismentioning

confidence: 99%

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Dopamine, vesicular transporters, and dopamine receptor expression in rat major salivary glands

Tomassoni

Traini

Mancini

et al. 2015

American Journal of Physiology-Regulatory, Integrative and Comp

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The localization of dopamine stores and the expression and localization of dopamine (DAT) and vesicular monoamine transporters (VMAT) type-1 and -2 and of dopamine D1-like and D2-like receptor subtypes were investigated in rat submandibular, sublingual, and parotid salivary glands by HPLC with electrochemical detection, as well as immunochemical and immunohistochemical techniques. Male Wistar rats of 2 mo of age were used. The highest dopamine levels were measured in the parotid gland, followed by the submandibular and sublingual glands. Western blot analysis revealed DAT, VMAT-1, VMAT-2, and dopamine receptors immunoreactivity in membrane preparations obtained from the three glands investigated. Immunostaining for dopamine and transporters was developed within striated ducts. Salivary glands processed for dopamine receptors immunohistochemistry developed an immunoreaction primarily in striated and excretory ducts. In the submandibular gland, acinar cells displayed strong immunoreactivity for the D2 receptor, while cells of the convoluted granular tubules were negative for both D1-like and D2-like receptors. Parotid glands acinar cells displayed the highest immunoreactivity for both D1 and D2 receptors compared with other salivary glands. The above localization of dopamine and dopaminergic markers investigated did not correspond closely with neuron-specific enolase (NSE) localization. This indicates that at least in part, catecholamine stores and dopaminergic markers are independent from glandular innervation. These findings suggest that rat major salivary glands express a dopaminergic system probably involved in salivary secretion. The stronger immunoreactivity for dopamine transporters and receptors in striated duct cells suggests that the dopaminergic system could regulate not only quality, but also volume and ionic concentration of saliva.

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Dopamine, vesicular transporters and dopamine receptor expression and localization in rat thymus and spleen

Cited by 43 publications

References 45 publications

Nerve Driven Immunity: Noradrenaline and Adrenaline

Nerve Driven Immunity: Noradrenaline and Adrenaline

Stimulation of Dopamine Receptor D5 Expressed on Dendritic Cells Potentiates Th17-Mediated Immunity

Dopamine, vesicular transporters, and dopamine receptor expression in rat major salivary glands

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