Dopaminergic excitation of the goat carotid body is mediated by the serotonin type 3 receptor subtype

Herman, Jay K.; O’Halloran, Ken D.; Janssen, P. L.; Bisgard, G. E.

doi:10.1016/s1569-9048(03)00088-0

Cited by 9 publications

(7 citation statements)

References 31 publications

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“…It has also been reported that carotid sinus nerve activity was augmented by the application of domperidone, a dopamine D 2 receptor antagonist (Iturriaga et al 1994;Herman et al 2003). Short-term hypoxia-induced TH may lead to the synthesis of dopamine to reduce chemosensory afferent nerve activity.…”

Section: Discussionmentioning

confidence: 92%

Short-term Hypoxia Increases Tyrosine Hydroxylase Immunoreactivity in Rat Carotid Body

Kato

Yamaguchi-Yamada

Yamamoto

2010

J Histochem Cytochem.

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S U M M A R Y Neurochemical and morphological changes in the carotid body are induced by chronic hypoxia, leading to regulation of ventilation. In this study, we examined the time courses of changes in immunohistochemical intensity for tyrosine hydroxylase (TH) and cellular volume of glomus cells in rats exposed to hypoxia (10% O 2 ) for up to 24 hr. Grayscale intensity for TH immunofluorescence was significantly increased in rats exposed to hypoxia for 12, 18, and 24 hr compared with control rats (p,0.05). The transectional area of glomus cells was not significantly different between experimental groups. The TH fluorescence intensity of the glomus cells exhibited a strong negative correlation with the transectional area in control rats (Spearman's ρ 5 20.70). This correlation coefficient decreased with exposure time, and it was lowest for the rats exposed to hypoxia for 18 hr (ρ 5 20.18). The histogram of TH fluorescence intensity showed a single peak in control rats. The peaks were gradually shifted to the right and became less pronounced in hypoxia-exposed rats, suggesting that a hypoxiainduced increase in TH immunoreactivity occurred uniformly in glomus cells. In conclusion, this study demonstrates that short-term hypoxia induces an increase in TH protein expression in rat carotid body glomus cells. (J Histochem Cytochem 58:839-846, 2010)

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Section: Discussionmentioning

confidence: 92%

Short-term Hypoxia Increases Tyrosine Hydroxylase Immunoreactivity in Rat Carotid Body

Kato

Yamaguchi-Yamada

Yamamoto

2010

J Histochem Cytochem.

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“…Injections given to inhibit the CCs included the following: (1) dopamine hydrochloride (5 to 10 g/kg) 24 ; (2) hyperoxic lactated Ringer's solution 25 ; and (3) serial injections of tropisetron (5 to 10 g/kg) and dopamine (5 to 10 g/kg). 26 Importantly, dopamine has not been shown to sensitize the carotid baroreceptors 27 and does not cross the blood-brain barrier. 28 Thirty-second isovolumetric normoxic Ringer's infusions were used as negative control injections to confirm that the cardiovascular response was not attributable to baroreceptor or thermal stimulation, or behavioral artifact.…”

Section: Close-carotid Injectionsmentioning

confidence: 99%

Carotid Chemoreceptor Modulation of Regional Blood Flow Distribution During Exercise in Health and Chronic Heart Failure

Stickland

Miller

Smith

et al. 2007

Circulation Research

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Abstract-Previous work has shown sensitization of carotid chemoreceptor (CC) responsiveness during exercise as well as in chronic heart failure (CHF). Accordingly, we hypothesized that the CCs contribute to the sympathetic restraint of skeletal muscle blood flow during exercise and CHF. We examined the effect of transient CC inhibition on total (Con T ) and hindlimb (Con L ) conductance, and blood pressure at rest and during exercise (2.5 miles per hour, 5% grade) in chronically instrumented dogs. Via a carotid arterial catheter, CCs were inhibited using dopamine (5 to 10 g/kg) or hyperoxic lactated Ringer's solution. Although vasodilation did not occur with CC inhibition in resting healthy dogs, CC inhibition during exercise caused an immediate vasodilatory response (increase in Con T and Con L and decrease in blood pressure). When comparing the peak Con L response from CC inhibition versus ␣-adrenergic blockade (phentolamine), we found that the CCs accounted for approximately one-third of the total sympathetic restraint during exercise. CHF was then induced by chronic rapid cardiac pacing and characterized by impaired cardiac function, enhanced chemosensitivity, and greater sympathetic restraint at rest and during exercise. In contrast to healthy dogs, CC inhibition in resting CHF dogs produced vasodilation, whereas a similar vasodilatory response was observed during exercise in CHF as compared with healthy dogs. The vasodilation following CC inhibition during exercise and in CHF was abolished with ␣-adrenergic blockade and was absent in healthy exercising animals after carotid body denervation. These results establish an important role for the CCs in cardiovascular control in the healthy animal during exercise and in the CHF animal both at rest and during exercise. Key Words: chemosensitivity Ⅲ sympathetic nervous activity Ⅲ exercise Ⅲ chronic heart failure Ⅲ blood flow D uring exercise, sympathetic vasoconstrictor activity increases, resulting in vasoconstriction in the gut and kidneys and will compete with local vasodilatory influences to constrain locomotor limb blood flow during exercise 1,2 to maintain blood pressure. 3 It is generally assumed that the increased sympathetic nervous activity (SNA) during exercise is caused by feedforward mechanisms such as central command and feedback from muscle metaboreceptors, muscle mechanoreceptors, and/or a resetting of systemic baroreceptors. 3 We propose that the tonic sensory input from the carotid chemoreceptors (CCs) might also be an important source of exercise-induced sympathetic vasoconstrictor activity.The CCs are traditionally thought to be the major oxygen sensors in the body, and their stimulation is assumed to cause a reflex-mediated increase in ventilation. However, CC stimulation also elicits significant increases in sympathetic vasoconstrictor outflow. 4,5 Importantly, the CCs are sensitive to a variety of stimuli in addition to oxygen, including, metabolic acidosis, 6 norepinephrine, 7 potassium, 8 glucose, 9 and angiotensin II, 10 all of which c...

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“…Serotonin modifies sensitivity to pheromone serotonin receptors (Bermudez et al, 1992;Tierney, 2001) or different levels of a common initial second messenger (Teshiba et al, 2001). At high concentrations, serotonin could also be activating other biogenic amine receptors (Herman et al, 2003). Different concentrations of serotonin in the AL could also differently affect synapses of the three types of neurons in the AL: olfactory receptor neurons, local interneurons and projection neurons.…”

Section: Serotonin Modulates Sensitivity To Bombykolmentioning

confidence: 99%

Serotonin modifies the sensitivity of the male silkmoth to pheromone

Gatellier

Nagao

Kanzaki

2004

Journal of Experimental Biology

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Serotonin is known to modulate the response of neuronal populations in the primary olfactory center of the moth olfactory system, the antennal lobe. Here, we analyzed the effects of serotonin on the behavior related to the restricted pheromone olfactory pathway of the male silkmoth, Bombyx mori. In order to understand the effects of serotonin at the behavioral level, we applied serotonin (10 -5 ·mol·l -1 , 10 -4 ·mol·l -1 and 10 -3 ·mol·l -1 ) to the brain and found that 10 -4 ·mol·l -1 serotonin increases the sensitivity to female pheromone whereas 10 -3 ·mol·l -1 serotonin had the opposite effect. Levels of serotonin in the brain were determined using HPLC with electrochemical detection. Inhibitory effects were observed after applying the serotonin antagonists mianserin (10 -4 ·mol·l -1 ) and ketanserin (10 -3 ·mol·l -1 ). Additionally, we quantified the circadian variation of serotonin in the brain using HPLC with electrochemical detection. Further, this variation correlated well with a circadian variation of the male sensitivity to pheromone. These results show that the serotonin-related enhancement of neuronal responses at the antennal lobe level is expressed at the behavioral level as a modulation of pheromone sensitivity and that the circadian variation of serotonin levels in the brain correlates with changes in the moth's pheromone sensitivity.

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Dopaminergic excitation of the goat carotid body is mediated by the serotonin type 3 receptor subtype

Cited by 9 publications

References 31 publications

Short-term Hypoxia Increases Tyrosine Hydroxylase Immunoreactivity in Rat Carotid Body

Short-term Hypoxia Increases Tyrosine Hydroxylase Immunoreactivity in Rat Carotid Body

Carotid Chemoreceptor Modulation of Regional Blood Flow Distribution During Exercise in Health and Chronic Heart Failure

Serotonin modifies the sensitivity of the male silkmoth to pheromone

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