Dopaminergic induction of human dental pulp stem cells by photobiomodulation: comparison of 660nm laser light and polychromatic light in the nir

Yurtsever, Merve Çapkın; Kiremitçi, Arlin; Gümüşderelı́oğlu, Menemşe

doi:10.1016/j.jphotobiol.2019.111742

Cited by 11 publications

(8 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among these 69 articles relevant to both PBM and PD, 31 basic researches were identified. Of which, 6 in vitro studies 39,40,[45][46][47][48] 24 in vivo studies, and a Monte Carlo simulation study were included. 73 Nine articles of clinical research including 8 articles of clinical studies 37,42,[74][75][76][77][78][79] and a clinical trial 43 were found.…”

Section: Resultsmentioning

confidence: 99%

“…As for the signal pathway involved in PD, Gu 47 demonstrated that PBM with He-Ne laser protected MPP+ exposed human dopaminergic neuroblastoma cells from MPP+-induced neurotoxicity via upregulation of extracellular signal-regulated kinases/cAMP-response element binding protein/vesicular monoamine transporter 2 pathway. Furthermore, in view of possible application of PBM on stem cell therapy for PD, Yurtsever 46 showed that PBM increased expression of dopaminergic neuron protective protein mRNAs in human dental pulp stem cells. However, no functional characteristic of dopaminergic neurons was developed.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Progress of Photobiomodulation for Parkinson’s Disease

Tsai¹

2022

SOJCEM

View full text Add to dashboard Cite

Parkinson’s disease (PD) is a neurodegenerative disease with global burden. The mechanisms and therapeutic effects of photobiomodulation (PBM) correspond to main mechanisms in the pathogenesis of PD. Numerous research results applying PBM for PD were published during the past two decades. Although several systematic review or review articles provided complete introduction, they are either mainly basic research or clinical research, and the year of the article publication is up to 2020. Comprehensive systematic review or review articles containing basic and clinical studies including those published articles is lacking. Hence, this systematic review aimed to include both basic and clinical studies published up to 2022. Results were obtained by retrieving articles from PubMed with the intersection of the articles derived from the terms of PBM synonyms and Parkinson’s disease followed by exclusion. Sixty-nine articles were included ultimately. Among them, 40 original articles were identified, which were composed of 31 basic research and 9 original articles of clinical research. Twenty-one review articles, a systematic review with focused content on PD, and 7 review articles with the term PD under general illustration of PBM were presented. Mechanisms regarding the therapeutic effects of PBM on the vitro studies were reviewed. Positive outcomes on motor symptoms after PBM treatments were shown in most in vivo and clinical studies. The immunohistochemical examination of in vivo studies reflect the therapeutic effects of PBM on the preservation even reverse of the pathogenic insults of PD on the in vitro studies. The most frequently used wavelength among original articles included was 670nm. Considering the acceptability of PBM for patients with PD, noninvasive transcranial PBM (tPBM) had crucial roles in respect to invasive intracerebral PBM. To match the penetration depth reaching deep brain target, Substantia nigra pars compacta, in human brains of patients with PD, the wavelength 810nm might match the need in the clinical setting of tPBM. More future clinical studies were needed. In conclusion, therapeutic approaches applying PBM for PD are promising. Recent studies revealed positive outcomes. Future clinical practices containing PBM are to be expected.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Progress of Photobiomodulation for Parkinson’s Disease

Tsai¹

2022

SOJCEM

View full text Add to dashboard Cite

show abstract

“…They can express markers of nerve lineage [ 50 , 51 ], such as low-affinity nerve growth factor receptor p75, intermediate filament nestin, and glial fibrillary acidic protein (GFAP), and more mature markers of nerve lineage such as β-III tubulin and nuclear antigen, which also reflects their origin and high heterogeneity [ 52 , 53 ]. Of course, a large number of studies have shown that it can differentiate into DAergic neuron-like cells [ 10 , 16 , 54 , 55 ]. In addition, DPSCs can also produce and secrete neurotrophic factors, induce axon guidance, and differentiate into functionally active neurons, which also illustrates great potential for cell transplantation therapy for PD.…”

Section: Dental Pulp Stem Cells Therapy For Pdmentioning

confidence: 99%

“…However, other researchers have found that they may only differentiate into premature DAergic neurons rather than functional and mature DAergic neurons [ 62 ]. These contradictory findings led a team recently to study the differentiation into dopaminergic neurons of DPSCs under different chemical and photobiological regulation conditions [ 55 ]. Their results showed that photobiological regulation can increase the expression of dopaminergic neuron protective protein mRNAs in DPSCs under certain conditions.…”

Section: Dental Pulp Stem Cells Therapy For Pdmentioning

confidence: 99%

The potential therapy with dental tissue-derived mesenchymal stem cells in Parkinson’s disease

et al. 2021

View full text Add to dashboard Cite

Parkinson’s disease (PD), the second most common neurodegenerative disease worldwide, is caused by the loss of dopaminergic (DAergic) neurons in the substantia nigra resulting in a series of motor or non-motor disorders. Current treatment methods are unable to stop the progression of PD and may bring certain side effects. Cell replacement therapy has brought new hope for the treatment of PD. Recently, human dental tissue-derived mesenchymal stem cells have received extensive attention. Currently, dental pulp stem cells (DPSCs) and stem cells from human exfoliated deciduous teeth (SHED) are considered to have strong potential for the treatment of these neurodegenerative diseases. These cells are considered to be ideal cell sources for the treatment of PD on account of their unique characteristics, such as neural crest origin, immune rejection, and lack of ethical issues. In this review, we briefly describe the research investigating cell therapy for PD and discuss the application and progress of DPSCs and SHED in the treatment of PD. This review offers significant and comprehensive guidance for further clinical research on PD.

show abstract

“…Therefore, DPSCs are valuable and crucial MSC sources for tissue engineering and regenerative medicine studies [43]. Dental pulp stem cells involve neural crest stem cells and they have potential to differentiate into neurogenic, adipogenic, chondrogenic, osteogenic cell types [44,45]. The studies which include pectin biomolecule and MSC interaction is generally based on bioscaffolds.…”

mentioning

confidence: 99%

Viability and Morphology of Human Dental Pulp Stem Cells in the Presence of Citrus Pectin

Ayar¹,

Yurtsever²

2020

Natural and Applied Sciences Journal

View full text Add to dashboard Cite

Pectin is a galacturonic acid rich heteropolysaccharide which regulates plant cell metabolism. Plenty of fresh fruits and fruit pomaces from fruit juice production can be used as a raw material in commercial pectin production. Pectin occupies a large global market size especially in food industry and the utilization of waste materials for obtaining pectin molecules as a high value-added product makes it very favorite industrial material. Besides food industry, pectin is gaining attention in tissue engineering and drug development studies. In this study, the effects of citrus pectin on viability and morphology of human dental pulp stem cell (hDPSC) were investigated. The cells were cultured in the presence of pectin in culture medium (0.43, 0.85 and 1.7 mg/mL) for eight days. Resazurin application and MTT assay were applied on day one and eight for cellular viability. Cellular morphology was investigated by invert phase contrast microscope, live/dead cell staining and F-actin/nucleus immunofluorescence staining. MTT analysis results indicated that the viability of hDPSCs decreased significantly due to dissolved pectin in culture medium at applied concentrations. There was no significant morphological difference in the cells under invert phase contrast microscope and no significant staining difference in live/dead cell staining images. On the other hand, F-actin/nucleus staining showed that there were some condensed and crescent cell nuclei in the pectin applied groups when compared to the control groups which may be related to apoptosis. In conclusion, the viability of hDPSCs decreased and crescent cell nuclei formation was observed due to the presence of citrus pectin in the cell culture medium.

show abstract

Dopaminergic induction of human dental pulp stem cells by photobiomodulation: comparison of 660nm laser light and polychromatic light in the nir

Cited by 11 publications

References 40 publications

Progress of Photobiomodulation for Parkinson’s Disease

Progress of Photobiomodulation for Parkinson’s Disease

The potential therapy with dental tissue-derived mesenchymal stem cells in Parkinson’s disease

Viability and Morphology of Human Dental Pulp Stem Cells in the Presence of Citrus Pectin

Contact Info

Product

Resources

About