Dosage Compensation via Transposable Element Mediated Rewiring of a Regulatory Network

Ellison, Chris; Bachtrog, Doris

doi:10.1126/science.1239552

Cited by 177 publications

(195 citation statements)

References 49 publications

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“…1B). Previous studies have found that newly evolved sex chromosomes can rapidly acquire DCC-binding sites through amplification of simple GA dinucleotide repeats that approximate the MSL recognition element (MRE) or domestication of MRE-bearing transposable elements Ellison and Bachtrog 2013;Zhou et al 2013).…”

Section: Rox Orthologs Bind the X Chromosomementioning

confidence: 99%

Rapid evolutionary turnover underlies conserved lncRNA–genome interactions

et al. 2016

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Many long noncoding RNAs (lncRNAs) can regulate chromatin states, but the evolutionary origin and dynamics driving lncRNA-genome interactions are unclear. We adapted an integrative strategy that identifies lncRNA orthologs in different species despite limited sequence similarity, which is applicable to mammalian and insect lncRNAs. Analysis of the roX lncRNAs, which are essential for dosage compensation of the single X chromosome in Drosophila males, revealed 47 new roX orthologs in diverse Drosophilid species across ∼40 million years of evolution. Genetic rescue by roX orthologs and engineered synthetic lncRNAs showed that altering the number of focal, repetitive RNA structures determines roX ortholog function. Genomic occupancy maps of roX RNAs in four species revealed conserved targeting of X chromosome neighborhoods but rapid turnover of individual binding sites. Many new roX-binding sites evolved from DNA encoding a pre-existing RNA splicing signal, effectively linking dosage compensation to transcribed genes. Thus, dynamic change in lncRNAs and their genomic targets underlies conserved and essential lncRNA-genome interactions.

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Section: Rox Orthologs Bind the X Chromosomementioning

confidence: 99%

Rapid evolutionary turnover underlies conserved lncRNA–genome interactions

et al. 2016

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“…Recently, comparative studies of the mechanism in dosage compensation in Drosophila melanogaster, D. pseudoobscura and D. miranda demonstrated that two waves of TE amplification allowed the evolution of dosage compensation in neo-X-chromosomes (Ellison and Bachtrog, 2013;Chuong and Feschotte, 2013).…”

Section: Transposable Element (Te) Signalsmentioning

confidence: 99%

Review Polyploidy and epigenetic events in the evolution of Anura

Ml¹

2014

Genet. Mol. Res.

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ABSTRACT. This article reviews the polyploidy events that have long been demonstrated to play a role in the evolution of Anura, while also discussing the importance of epigenetic control of gene expression and diversity. Findings on Brazilian autopolyploid anurans, mainly of the genus Odontophrynus, obtained in previous studies on their cytogenetics, chromatin ultrastructure, and molecular gene regulation are discussed here. Our data on genome duplication and on epigenetic events were analyzed here regarding phylogenetic trees, including the classic 2R model for vertebrate evolution and the growing evidence of similar epigenetic mechanisms in animal and allopolyploid plants. We propose that polyploidy and epigenetic events led to rapid Anura diversity and speciation. Also, recent advances in molecular studies in other organisms led us to revisit some controversial models of evolution.

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“…Within the HASs, a GA-rich sequence motif, the MSL recognition element (MRE), is important for MSL-DCC targeting (Alekseyenko et al 2008;Straub et al 2008). Interestingly, in the related species Drosophila miranda, large autosomal fragments have been fused to a proto-X chromosome relatively recently and are apparently on their way to ''catch up'' with dosage compensation by newly acquiring high-affinity MRE sequences from transposon-derived precursor sequences (Alekseyenko et al 2013;Ellison and Bachtrog 2013).…”

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confidence: 99%

Structural basis of X chromosome DNA recognition by the MSL2 CXC domain during Drosophila dosage compensation

et al. 2014

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The male-specific lethal dosage compensation complex (MSL-DCC) selectively assembles on the X chromosome in Drosophila males and activates gene transcription by twofold through histone acetylation. An MSL recognition element (MRE) sequence motif nucleates the initial MSL association, but how it is recognized remains unknown. Here, we identified the CXC domain of MSL2 specifically recognizing the MRE motif and determined its crystal structure bound to specific and nonspecific DNAs. The CXC domain primarily contacts one strand of DNA duplex and employs a single arginine to directly read out dinucleotide sequences from the minor groove. The arginine is flexible when bound to nonspecific sequences. The core region of the MRE motif harbors two binding sites on opposite strands that can cooperatively recruit a CXC dimer. Specific DNA-binding mutants of MSL2 are impaired in MRE binding and X chromosome localization in vivo. Our results reveal multiple dynamic DNA-binding modes of the CXC domain that target the MSL-DCC to X chromosomes.

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Dosage Compensation via Transposable Element Mediated Rewiring of a Regulatory Network

Cited by 177 publications

References 49 publications

Rapid evolutionary turnover underlies conserved lncRNA–genome interactions

Rapid evolutionary turnover underlies conserved lncRNA–genome interactions

Review Polyploidy and epigenetic events in the evolution of Anura

Structural basis of X chromosome DNA recognition by the MSL2 CXC domain during Drosophila dosage compensation

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