Dose-dependent effect of Resveratrol on bladder cancer cells: Chemoprevention and oxidative stress

Stocco, Bianca; Toledo, Karina Alves; Salvador, Mirian; Paulo, Michele; Koyama, Natalia S.; Tolói, Maria Regina Torqueti

doi:10.1016/j.maturitas.2012.02.004

Cited by 46 publications

(35 citation statements)

References 30 publications

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“…The predominance of cytostatic HTC cells in groups of juice that grapes were treated with UV-C may be due to the effect of UV-C irradiation, which stimulates the production of resveratrol in postharvest grapes (Cantos et al, 2001). However, according to Stocco et al (2012), phytoalexin has protective effects on cells at low concentrations and can sensitize the cells at high concentrations.…”

Section: Resultsmentioning

confidence: 99%

Influence of processing and storage of integral grape juice (Vitis labrusca L.) on its physical and chemical characteristics, cytotoxicity, and mutagenicity in vitro

et al. 2017

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ABSTRACT. Integral grape juice is extracted from the grape through processes that allow the retention of their natural composition. However, due to the severity of some processes, fruit juices can undergo changes in their quality. The present study evaluated the cytotoxic and mutagenic effects of integral grape juice by a cytokinesis-blocked micronucleus assay in Rattus norvegicus hepatoma cells (HTC) in vitro. Vitis labrusca L. (variety Concord) were produced organically and by a conventional system, and their juice was extracted by a hot extraction process. The organic grapes were subjected to ultraviolet-type C radiation (UV-C). Experiments were performed after production and after 6 months in storage. Physicochemical analyses revealed that UV-C irradiation of 2 E. Düsman et al. (2): gmr16029670 organic grapes, the juice production process, and storage resulted in nutraceutical alterations. However, none of the juice concentrations were cytotoxic to HTC cells by the cytokinesis-blocked proliferation index results or were mutagenic, because the formation of micronucleated cells was not induced. In general, juice induced cell proliferation, possibly due to the presence of vitamins and sugar content (total soluble solid). The data increased the understanding of food technology and confirmed the quality and safety consumption of these juices. Genetics and Molecular Research 16

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Section: Resultsmentioning

confidence: 99%

Influence of processing and storage of integral grape juice (Vitis labrusca L.) on its physical and chemical characteristics, cytotoxicity, and mutagenicity in vitro

et al. 2017

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“…RV showed the beneficial antitumor bioactivity in HBC cells [13, 33, 34], but how RV metabolized and whether RV or its metabolites (sulfates or glucuronides) exerted the corresponding effect on bladder carcinoma have not been reported so far. In this content, we found that HBC T24 and EJ cells showed different sensitivity to RV.…”

Section: Discussionmentioning

confidence: 99%

Differential sensitivities of bladder cancer cell lines to resveratol are unrelated to its metabolic profile

Yang

et al. 2017

Oncotarget

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Resveratrol (RV) is a natural polyphenol compound with a wide range of activities, including inhibition of human bladder cancer (HBC) cell growth. Because RV is rapidly metabolized and has poor bioavailability, it is unclear whether the antitumor activity is due to RV or its metabolites. We therefore used liquid chromatography-mass spectroscopy, qRT-PCR, immunocytochemistry and western blotting to evaluate the metabolic profile and biotransformation of RV in the T24 and EJ HBC cell lines. Both T24 and EJ cells generated the same RV metabolite, RV monosulfate (RVS), and both exhibited upregulation of the RV-associated metabolic enzyme SULT1A1 (sulfotransferase). Despite these similarities, T24 cells were more sensitive to RV than EJ cells, yet T24 cells exhibited no sensitivity to an RVS mixture (84.13% RVS). Primary rat bladder epithelial cells showed no adverse effects when exposed to a therapeutic dose (100 μM) of RV. The differences in RV sensitivity between the two HBC cell lines did not reflect differences in the RV metabolic profile or SULT1A1 expression. Because RV exhibited stronger antitumor activity and better safety than RVS, we conclude that RV has significant therapeutic potential for HBC treatment, provided individual differences are considered during clinical research and application.

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“…The results indicated that resveratrol at the concentration of 2.5 µmol/L protected the ECV304 cells from the damage induced by oxidative stress. High concentration (>20 µmol/L) of resveratrol induced cell death and increased cell damage from oxidative stress [41]. Beside, Bai Y et al investigated the chemopreventive potential of resveratrol against T24 cells [42].…”

Section: Discussionmentioning

confidence: 99%

Comparision of Piceid and Resveratrol in Antioxidation and Antiproliferation Activities In Vitro

et al. 2013

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BackgroundThe clinic therapeutic effect of resveratrol is limited due to its low oral bioavailability. Piceid, a precursor of resveratrol, is the most abundant form of resveratrol in nature. A number of studies have hypothesized that piceid may have the same bioactivities like those of resveratrol. The aim of this work is to compare piceid with resveratrol in antioxidation and antiproliferation activities in vitro.MethodsThe antioxidative effects of resveratrol and piceid were evaluated by phenanthroline-Fe2+ method and H2O2-induced oxidative injury cell model. The antiproliferation effects were determined by MTT method in human liver tumor HepG2 cells, human breast cancer MDA-MB-231 cells and MCF-7 cells. The effects of resveratrol and piceid on the cell cycle and the apoptosis were evaluated by flow cytometry. Additionally, the uptake profiles of resveratrol and piceid in cancer cells were observed using fluorescence microscopy and clarified by LC-MS/MS.ConclusionPiceid exhibited higher scavenging activity against hydroxyl radicals than resveratrol in vitro. Resveratrol showed a significant protective effect against H2O2-induced cell damage. What is more, resveratrol had biphasic effects on tumor cells. Resveratrol and piceid only showed significant cytotoxicity on tumor cells at high concentration (≥50 µmol/L), while low concentration of resveratrol (<30 µmol/L) increased the cell viability. The principal effect of resveratrol and piceid on the viability of tumor cells was caused by the cell cycle arrest, while the effect on apoptosis was relatively minor. The reason that piceid showed lower biological activity than resveratrol at the same concentration was probably because piceid was more difficult in being uptaken by cells.

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Dose-dependent effect of Resveratrol on bladder cancer cells: Chemoprevention and oxidative stress

Cited by 46 publications

References 30 publications

Influence of processing and storage of integral grape juice (Vitis labrusca L.) on its physical and chemical characteristics, cytotoxicity, and mutagenicity in vitro

Influence of processing and storage of integral grape juice (Vitis labrusca L.) on its physical and chemical characteristics, cytotoxicity, and mutagenicity in vitro

Differential sensitivities of bladder cancer cell lines to resveratol are unrelated to its metabolic profile

Comparision of Piceid and Resveratrol in Antioxidation and Antiproliferation Activities In Vitro

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