Dose-dependent effects of caffeine in human Sertoli cells metabolism and oxidative profile: Relevance for male fertility

Dias, Tânia R.; Alves, Marco G.; Bernardino, Raquel L.; Martins, Ana D.; Moreira, Ana C.; Silva, Joaquina; Barros, Alberto; Sousa, Mário; Silva, Branca M.; Oliveira, Pedro F.

doi:10.1016/j.tox.2014.12.003

Cited by 80 publications

(71 citation statements)

References 57 publications

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“…Studies have shown that exposure of human cells to high levels of caffeine induced a prooxidant environment in the cells, leading to increased protein oxidation, while low levels of caffeine had no effect on the antioxidant capacity of cells [20]. It has been demonstrated that caffeine significantly increased BUN levels, resulting in the activation of xanthine oxidase which in turn, stimulated the oxidation of xanthine to uric acid, and generation of superoxide anion and H 2 O 2 [21].…”

Section: Discussionmentioning

confidence: 99%

Effects of chronic consumption of energy drinks on liver and kidney of experimental rats

Mansy¹,

Alogaiel²,

Hanafi³

et al. 2018

Trop. J. Pharm Res

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Section: Discussionmentioning

confidence: 99%

Effects of chronic consumption of energy drinks on liver and kidney of experimental rats

Mansy¹,

Alogaiel²,

Hanafi³

et al. 2018

Trop. J. Pharm Res

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“…Due to its gonadotoxic and pro-oxidant properties, caffeine possesses preferential targets on Sertoli cells and spermatogonia and appears to cause little harm to spermatids, differentiating and mature spermatozoa [7,24]. At high caffeine concentrations, these effects would result in a reduction of sperm concentrations in a similar fashion as other compounds do either directly or through the endocrine system.…”

Section: Discussionmentioning

confidence: 99%

“…Knowing the composition of energy drinks and the fact that they contain psychoactive substances with highly stimulating properties, an important factor to consider is the question of caffeine concentrations, which can vary between 50 mg per 250 mL can and 505 mg per 1 L bottle, which may result in poisoning or even overdose [4]. In addition to poisoning by caffeine, the consumption of energy drinks has been associated with seizures [5], strokes [6], and gonadotoxic effects [7]. A significant increase in the incidence of male infertility has been described in the literature worldwide, which generates questions about its causes.…”

Section: Introductionmentioning

confidence: 99%

Effects of energy drinks on biochemical and sperm parameters in Wistar rats

Schuchowsky

Schaefer

Salvador

et al. 2017

Nutrire

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Background: The present study evaluates the effects of energy drinks on the reproductive and biochemical parameters of adult male rats. Methods: A total of 40 male rats (Wistar) were exposed to an energy drink mixed with the drinking water for a period of 120 days. The animals were divided into four groups and exposed to increasing therapeutic doses (DT) of an energy drink, based on allometric extrapolation, resulting in values (mL/day) per animal of 250 g: DT1 2.36 mL, DT3 7.47 mL, and DT6 14.16 mL. The control group (CTRL) consumed water only. During the treatment, the rats were assessed for signs of toxicity. After treatment, the animals were sacrificed and their organs were weighed. Sperm parameters (motility, concentration, and morphology) were evaluated. The biochemical markers alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, lactic dehydrogenase, urea, creatinine, creatine phosphokinase, and creatine kinase MB fraction were measured, in addition to total cholesterol and testosterone. Results: There was a significant decrease (p < 0.05) in the concentration of sperm in the treated groups (DT1 8.5 ± 0.7; DT3 7.2 ± 0.9; DT6 8.4 ± 0.9) compared to the control group (12.3 ± 1.2). No difference was observed with respect to relative weights of the animals' organs, water consumption, signs of toxicity, behavioral changes, biochemical markers, and sperm motility and morphology. Conclusion: The long-term consumption of energy drinks interferes negatively with sperm concentration, without affecting sperm motility and morphology or altering the hepatic, cardiac, or renal functions.

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“…More recently, leptin 59 has been reported to be also produced in other tissues [4]. The plasma 60 concentration of leptin tends to be increased in most obese individuals 61 and positively correlated with total body fat [5,6], with the exception 62 of the rare individuals with congenital leptin deficiency [7]. In fact, lep- 63 tin possesses anti-obesity functions, based on its ability to suppress ap- 64 petite and decrease body weight and adiposity [2].…”

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confidence: 99%

Leptin modulates human Sertoli cells acetate production and glycolytic profile: a novel mechanism of obesity-induced male infertility?

Martins

Moreira

Sá

et al. 2015

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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Human feeding behavior and lifestyle are gradually being altered, favoring the development of metabolic diseases, particularly type 2 diabetes and obesity. Leptin is produced by the adipose tissue acting as a satiety signal. Its levels have been positively correlated with fat mass and hyperleptinemia has been proposed to negatively affect male reproductive function. Nevertheless, the molecular mechanisms by which this hormone affects male fertility remain unknown. Herein, we hypothesize that leptin acts on human Sertoli cells (hSCs), the "nurse cells" of spermatogenesis, altering their metabolism. To test our hypothesis, hSCs were cultured without or with leptin (5, 25 and 50ng/mL). Leptin receptor was identified by qPCR and Western blot. Protein levels of glucose transporters (GLUT1, GLUT2 and GLUT3), phosphofructokinase, lactate dehydrogenase (LDH) and monocarboxylate transporter 4 (MCT4) were determined by Western Blot. LDH activity was assessed and metabolite production/consumption determined by proton nuclear magnetic resonance. Oxidative damage was evaluated by assessing lipid peroxidation, protein carbonilation and nitration. Our data shows that leptin receptor is expressed in hSCs. The concentration of leptin found in lean, healthy patients, upregulated GLUT2 protein levels and concentrations of leptin found in lean and obese patients increased LDH activity. Of note, all leptin concentrations decreased hSCs acetate production illustrating a novel mechanism for this hormone action. Moreover, our data shows that leptin does not induce or protect hSCs from oxidative damage. We report that this hormone modulates the nutritional support of spermatogenesis, illustrating a novel mechanism that may be linked to obesity-induced male infertility.

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Dose-dependent effects of caffeine in human Sertoli cells metabolism and oxidative profile: Relevance for male fertility

Cited by 80 publications

References 57 publications

Effects of chronic consumption of energy drinks on liver and kidney of experimental rats

Effects of chronic consumption of energy drinks on liver and kidney of experimental rats

Effects of energy drinks on biochemical and sperm parameters in Wistar rats

Leptin modulates human Sertoli cells acetate production and glycolytic profile: a novel mechanism of obesity-induced male infertility?

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