Dose-Response and Time-Course of a-Tocoferol Mediating the Cytoprotection Of Dental Pulp Cells Against Hydrogen Peroxide

Vargas, Fernanda da Silveira; Soares, Diana Gabriela; Basso, Fernanda Gonçalves; Hebling, Josimeri; Costa, Carlos Alberto de Souza

doi:10.1590/0103-6440201302434

Cited by 14 publications

(9 citation statements)

References 24 publications

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“…Prebleaching administration of anti-inflammatory drugs and topical application of sodium fluoride and potassium nitrate desensitizing agents as well as the use of antioxidant molecules have been widely assessed. 8 9 10 11 12 13 14 15 Since the pathway for pulp tissue damage following tooth bleaching involves oxidative damage to pulp cells, therapies using antioxidant agents have been recognized as interesting alternatives for preventing the occurrence of conditions leading to intense oxidative stress. 13 15 Antioxidant agents have been successfully used to restore optimal bonding after bleaching treatment.…”

Section: Introductionmentioning

confidence: 99%

“… 8 9 10 11 12 13 14 15 Since the pathway for pulp tissue damage following tooth bleaching involves oxidative damage to pulp cells, therapies using antioxidant agents have been recognized as interesting alternatives for preventing the occurrence of conditions leading to intense oxidative stress. 13 15 Antioxidant agents have been successfully used to restore optimal bonding after bleaching treatment. 16 17 18 In addition, previous studies have demonstrated that antioxidant molecules, such as alpha-tocoferol and ascorbic acid (AA), were capable of preventing HP-mediated pulp cell damage.…”

Section: Introductionmentioning

confidence: 99%

“… 16 17 18 In addition, previous studies have demonstrated that antioxidant molecules, such as alpha-tocoferol and ascorbic acid (AA), were capable of preventing HP-mediated pulp cell damage. 11 12 13 15 According to the authors, these antioxidant agents may inactivate the extracellular reactive oxygen species (ROS) from bleaching gels, as well as the intracellular ROS released by cells undergoing oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant therapy enhances pulpal healing in bleached teeth

et al. 2016

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ObjectivesThe purpose of this study was to evaluate the histopathological effects of an antioxidant therapy on the pulp tissue of rat teeth exposed to a bleaching gel with 35% hydrogen peroxide.Materials and MethodsForty rats were subjected to oral ingestion by gavage of distilled water (DW) or ascorbic acid (AA) 90 min before the bleaching therapy. For the bleaching treatment, the agent was applied twice for 5 min each to buccal surfaces of the first right mandibular molars. Then, the animals were sacrificed at 6 hr, 24 hr, 3 day, or 7 day post-bleaching, and the teeth were processed for microscopic evaluation of the pulp tissue.ResultsAt 6 hr, the pulp tissue showed moderate inflammatory reactions in all teeth of both groups. In the DW and AA groups, 100% and 80% of teeth exhibited pulp tissue with significant necrosis and intense tissue disorganization, respectively. At 24 hr, the AA-treated group demonstrated a greater regenerative capability than the DW group, with less intense inflammatory reaction and new odontoblast layer formation in 60% of the teeth. For up to the 7 day period, the areas of pulpal necrosis were replaced by viable connective tissue, and the dentin was underlined by differentiated odontoblast-like cells in most teeth of both groups.ConclusionsA slight reduction in initial pulpal damage during post-bleaching was promoted by AA therapy. However, the pulp tissue of AA-treated animals featured faster regenerative potential over time.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antioxidant therapy enhances pulpal healing in bleached teeth

et al. 2016

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“…Then, 16 studies were excluded, and the reasons for their exclusion can be found in Figure 1 and Supplementary Data 1 [ 32 33 34 35 36 37 38 39 40 41 42 43 44 ]. Twenty-seven studies meeting the inclusion criteria were selected for qualitative analysis [ 1 3 6 11 12 17 23 24 25 26 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 ].…”

Section: Resultsmentioning

confidence: 99%

Can different agents reduce the damage caused by bleaching gel to pulp tissue? A systematic review of basic research

Batista,

dos Reis-Prado,

Chaves

et al. 2023

Restor Dent Endod

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Objectives This study aimed to investigate the effectiveness of different topical/systemic agents in reducing the damage caused by bleaching gel to pulp tissue or cells. Materials and Methods Electronic searches were performed in July 2023. In vivo and in vitro studies evaluating the effects of different topical or systemic agents on pulp inflammation or cytotoxicity after exposure to bleaching agents were included. The risk of bias was assessed. Results Out of 1,112 articles, 27 were included. Nine animal studies evaluated remineralizing/anti-inflammatories agents in rat molars subjected to bleaching with 35%–38% hydrogen peroxide (HP). Five of these studies demonstrated a significant reduction in inflammation caused by HP when combined with bioglass or MI Paste Plus (GC America), or following KF-desensitizing or Otosporin treatment ( n = 3). However, orally administered drugs did not reduce pulp inflammation ( n = 4). Cytotoxicity ( n = 17) was primarily assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay on human dental pulp cells and mouse dental papilla Cell-23 cells. Certain substances, including sodium ascorbate, butein, manganese chloride, and peroxidase, were found to reduce cytotoxicity, particularly when applied prior to bleaching. The risk of bias was high in animal studies and low in laboratory studies. Conclusions Few in vivo studies have evaluated agents to reduce the damage caused by bleaching gel to pulp tissue. Within the limitations of these studies, it was found that topical agents were effective in reducing pulp inflammation in animals and cytotoxicity. Further analyses with human pulp are required to substantiate these findings. Trial Registration PROSPERO Identifier: CRD42022337192

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“…9,10 The anti-inflammatory substances and low level laser therapy used have been tested and can promote reduction of pulp damage after dental bleaching, but the results are not conclusive. 9,14 The reduction of the bleaching gel concentration used in in-office bleaching is also an option to minimize the damage. 7,8,31 However, this product requires repeated sessions containing lower H 2 O 2 concentration, 6 a procedure which may also increase the damage to pulp tissue.…”

Section: ;28:e20190384 7/10mentioning

confidence: 99%

Experimental gel containing bioactive glass-ceramic to minimize the pulp damage caused by dental bleaching in rats

et al. 2020

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Experimental gel containing bioactive glass-ceramic to minimize the pulp damage caused by dental bleaching in rats Objectives: This study evaluated if the use of a bioactive glass-ceramicbased gel, named Biosilicate (BS), before, after or mixed with bleaching gel, could influence the inflammation of the dental pulp tissue of rats' molars undergoing dental bleaching with hydrogen peroxide (H 2 O 2). Methodology: The upper molars of Wistar rats (Rattus norvegicus, albinus) were divided into Ble: bleached (35% H 2 O 2 , 30-min); Ble-BS: bleached and followed by BS-based gel application (20 min); BS-Ble: BS-based gel application and then bleaching; BS/7d-Ble: BS-based gel applications for 7 days and then bleaching; Ble+BS: blend of H 2 O 2 with BS-based gel (1:1, 30-min); and control: placebo gel. After 2 and 30 days (n=10), the rats were euthanized for histological evaluation. The Kruskal-Wallis and Dunn statistical tests were performed (P<0.05). Results: At 2 days, the Ble and Ble-BS groups had significant alterations in the pulp tissue, with an area of necrosis. The groups with the application of BS-based gel before H 2 O 2 had moderate inflammation and partial disorganization in the occlusal third of the coronary pulp and were significantly different from the Ble in the middle and cervical thirds (P<0.05). The most favorable results were observed in the Ble+BS, which was similar to the control in all thirds of the coronary pulp (P>0.05). At 30 days, the pulp tissue was organized and the bleached groups presented tertiary dentin deposition. The Ble group had the highest deposition of tertiary dentin, followed by the Ble-BS, and both were different from control (P<0.05). Conclusion: A single BS-based gel application beforehand or BSbased gel blended with a bleaching gel minimize the pulp damage induced by dental bleaching.

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Dose-Response and Time-Course of a-Tocoferol Mediating the Cytoprotection Of Dental Pulp Cells Against Hydrogen Peroxide

Cited by 14 publications

References 24 publications

Antioxidant therapy enhances pulpal healing in bleached teeth

Antioxidant therapy enhances pulpal healing in bleached teeth

Can different agents reduce the damage caused by bleaching gel to pulp tissue? A systematic review of basic research

Experimental gel containing bioactive glass-ceramic to minimize the pulp damage caused by dental bleaching in rats

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