Dosing Aluminum Chloride To Control<i>Microthrix parvicella</i>

Paris, Stefania; Lind, George; Lemmer, Hilde; Wilderer, P.A.

doi:10.1002/aheh.200400577

Cited by 20 publications

(10 citation statements)

References 8 publications

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“…The tendency of the tested sludge to foam was reduced by all the methods (Table 1). Improvement of sludge settling characteristics and decrease in foaming tendency were previously noticed by Paris et al (2005) during the aluminum chloride dosing. Also a study by Nielsen et al (2005) confirmed that a dosage of polyaluminium salts can be an effective control for the growth of Microthrix parvicella in activated sludge and partly explained the mechanism of PAX14 action.…”

Section: Efficiency Of Methods For Sludge Bulking Controlsupporting

confidence: 53%

Selection of methods for activated sludge bulking control using a molecular biology technique combined with respirometric tests

Miłobędzka¹,

Muszyński²

2016

bta

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An excessive proliferation of certain groups of filamentous bacteria results in activated sludge (AS) bulking and foaming. Studies on the efficiency of specific and non-specific methods for AS bulking control were conducted. Polyaluminium compounds (PAX16 and PAX18) were used as specific methods, whereas changes in pH and in temperature were tested among non-specific methods. Floatation of AS was determined by the Alka-Seltzer-test, ability of AS to settle was measured as sludge volume index. Oxygen uptake rate tests were carried out to determine respiratory activity of AS. Fluorescence in situ hybridisation was applied to identify and quantify filamentous bacteria in AS, which cannot be obtained by the conventional method. Two dominant groups of filamentous bacteria-phylum Chloroflexi and genus Microthrix were found in AS samples, the latter was the causative agent for bulking. The nonspecific methods for sludge bulking control (pH/temperature changes) improved the settling properties, however they did not eliminate filamentous bacteria and in some cases decreased sludge respiratory activity. PAX16 (5.7 mg/kg SS) was the most efficient control strategy for bulking caused by Microthrix-it improved settling properties without increasing tendency to foam and did not decrease respiratory activity of AS.

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Section: Efficiency Of Methods For Sludge Bulking Controlsupporting

confidence: 53%

Selection of methods for activated sludge bulking control using a molecular biology technique combined with respirometric tests

Miłobędzka¹,

Muszyński²

2016

bta

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“…In contrast, morphotype M. parvicella turned out to be one single phylogenetic entity. Therefore, a selective control strategy against M. parvicella (i.e., aluminum dosage) might be effective throughout the world, which is verified by various reports ( Eikelboom et al, 1998 ; Nielsen et al, 2005 ; Paris et al, 2005 ). Conventional activated sludge microscopy was found to be a successful tool to identify this organism.…”

Section: Discussionmentioning

confidence: 62%

Filamentous Scum Bacteria in Activated Sludge Plants: Detection and Identification Quality by Conventional Activated Sludge Microscopy versus Fluorescence In Situ Hybridization

Müller¹,

Schade²,

Lemmer³

2007

Water Environment Research

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Detection of filamentous bacteria morphotypes involved in scum formation in activated sludge wastewater treatment plants by conventional sludge microscopy is often doomed to fail because of morphological and taxonomical variations. The aim of this study is to compare detection, identification, and quantification quality of filamentous ''scum bacteria'' found by conventional activated sludge microscopy and fluorescence in situ hybridization (FISH). In the case of filamentous Microthrix parvicella and Eikelboom morphotypes 1863 and 1851, conventional activated sludge microscopy and FISH results correspond well. In contrast, conventional activated sludge microscopy overlooks nocardioform actinomycete and type 1863 single cells. On the other hand, FISH underestimates filamentous nocardioform actinomycetes and morphotypes 0041/0675 or 0092 because of insufficient cell wall permeability or because of their taxonomic variability, with a resulting inadequacy of previously published probes. Nostocoida limicola morphotype results are still inconclusive because of low bacteria numbers being available in situ and the enormous taxonomic variability within this group. Water Environ. Res., 79, 2274Res., 79, (2007.

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“…Polyaluminium chloride (PAX) treatments have been proved to be an effective measure to suppress Microthrix growth in WWTP Paris et al, 2005). To exclude possible PCR inhibition by PAX, an additional inhibition experiment was performed using different PAX-14 concentrations.…”

Section: Determination Of Pcr Inhibition By Polyaluminium Chloridementioning

confidence: 99%

Development and evaluation of a TaqMan duplex real-time PCR quantification method for reliable enumeration of Candidatus Microthrix

Vanysacker

Denis

Roels

et al. 2014

Journal of Microbiological Methods

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Candidatus Microtrhix parvicella is one of the most common filamentous bacteria reported to be involved in bulking and foaming problems in activated sludge plants worldwide. In order to detect and quantify both M. parvicella and Microthrix calida by quantitative PCR (qPCR), primers targeting 16S rDNA genes were designed. The qPCR reaction was optimized by using the TaqMan technology and an internal positive control was included to ensure the absence of PCR inhibitors. A total of 29 samples originating from different wastewater treatment plants were analyzed and the results were compared by using conventional microscopy, fluorescent in situ hybridization and an existing SYBR Green-based assay. Our assay showed a 100% specificity for both M. parvicella and M. calida, a sensitivity of 2.93 × 10 9 to 29 copy numbers/reaction, an amplification efficiency of 93% and no PCR inhibition. By performing a spiking experiment including different Microthrix concentrations, recovery rates ranging from 65 to 98% were obtained. A positive correlation with the SYBR Green assay (R 2 = 0.85) was found and most of the samples were in accordance with the microscopical observation. In comparison with SYBR Green assay, the probe-based TaqMan assay had a much lower detection limit. Compared with microscopy, some samples had a lower or higher enumeration when using qPCR. In conclusion, a qPCR method is forwarded here that could be useful as an early warning tool for fast and reliable detection of Microthrix in for instance sludge bulking events.

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Dosing Aluminum Chloride To ControlMicrothrix parvicella

Cited by 20 publications

References 8 publications

Selection of methods for activated sludge bulking control using a molecular biology technique combined with respirometric tests

Selection of methods for activated sludge bulking control using a molecular biology technique combined with respirometric tests

Filamentous Scum Bacteria in Activated Sludge Plants: Detection and Identification Quality by Conventional Activated Sludge Microscopy versus Fluorescence In Situ Hybridization

Development and evaluation of a TaqMan duplex real-time PCR quantification method for reliable enumeration of Candidatus Microthrix

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