Double antibody solid-phase radioimmunoassay for staphylococcal enterotoxin A

Staphylococci in milk and milk products

“…However, methods have been developed which are more sensitive and rapid. These include radioimmunoassay (RIA) (Lindroth & Niskanen 1977;Miller et al 1978), enzyme-linked immunosorbent assay (ELISA) (Notermans et al 1978;Kuo & Silverman 1980), reversed passive haemagglutination assay (RPHA) (Morse & Mah 1967;Yamada et al 1977). They did not come into earlier routine use, however, for various technical reasons such as the requirement for very highly purified reagents which until recently were difficult to obtain and prohibitively expensive and also, in the case of RIA, the need for specialized equipment to measure the radioactive uptake of samples.…”

PREVENTION OF CROSS‐REACTIONS IN THE ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA) FOR THE DETECTION OF STAPHYLOCOCCUS AUREUS ENTEROTOXIN TYPE B IN CULTURE FILTRATES AND FOODS

“…Enzyme Linked Immunosorbent Assay (ELISA) (Notermans et al 1978;Simon and Terplan 1977;Fey 1978), Radioimmunoassay (RIA) (Miller e t al. 1978;Lindroth and Niskanen 1977;Pober and Silverman 1977;Robern et al 1978), Enzyme Immuno Assay (EIA) (Saunders and Bartlett 1977), Homogeneous Enzyme Immune Assay (Morita and Woodburn 1978) and Reversed Passive Hemagglutination Assay (RPHA) (Shinagawa et al 1977b). Some of these methods can be used after a greatly simplified extraction procedure.…”

“…Some of these methods can be used after a greatly simplified extraction procedure. To our know-ledge, however, most of these methods have so far only been applied to the quantification of purified toxin, that had been added to food (Robern et al 1978), or even t o food extracts (Lindroth and Niskanen 1977;Pober and Silverman 1977;Saunders and Bartlett 1977).…”

Comparative evaluation of different enzyme-linked immunosorbent assay systems for the detection of staphylococcal enterotoxins A, B, C, and D

“…The radioimmunosorbent assays (5,9,21,26,30,32,33) fulfill these requirements but have many well-known disadvantages. The enzyme-linked immunosorbent assay (ELISA) is equally sensitive and is gaining increasing credit in this field.…”