Double-chamber plethysmography <i>versus</i> oscillometry to detect baseline airflow obstruction in a model of asthma in two mouse strains

Boucher, Magali; Henry, Cyndi; Khadangi, Fatemeh; Dufour-Mailhot, Alexis; Bossé, Ynuk

doi:10.1080/01902148.2021.1979693

Cited by 10 publications

(10 citation statements)

References 49 publications

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“…It was characterized by increases in lung elastance ( H ) and tissue resistance ( G ). These results might appear inconsistent with our previous study (Boucher, Henry, Khadangi et al., 2021 ), in which only H was sightly but significantly increased by asthma in female mice. These inconsistencies are attributed to the presence (vs. the absence) of deep inflations before the measurement of respiratory mechanics.…”

Section: Discussioncontrasting

confidence: 99%

“…Mice of each sex were divided into two groups of 20 (Figure 1 ): one group exposed to 25 μL of saline (control mice) and one group exposed to 25 μL of 2 mg of house dust mite (HDM) extract ( Dermatophagoides pteronyssinus ; Greer, Lenoir, NC, USA) per millilitre of saline to induce pulmonary allergic inflammation (asthmatic mice) (Boucher, Henry, Dufour‐Mailhot et al., 2021 , 2021 ; Sahu et al., 2010 ). The endotoxin concentration was 47.3 EU per milligram of HDM extract.…”

Section: Methodsmentioning

confidence: 99%

“…Mice were weighed, and their respiratory mechanics were tested with the flexiVent (FX Module 2, SCIREQ, Montreal, QC, Canada) as described in our recent studies (Boucher, Dufour‐Mailhot et al., 2022 ; Boucher, Henry, Khadangi et al., 2021 ; Khadangi et al., 2021 , 2022 ). Briefly, they were anaesthetized and put under systemic analgesia using ketamine (100 mg/kg) and xylazine (10 mg/kg).…”

Section: Methodsmentioning

confidence: 99%

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More airway smooth muscle in males versus females in a mouse model of asthma: A blessing in disguise?

Gill

Rojas‐Ruiz

Boucher

et al. 2023

Experimental Physiology

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Mouse models are helpful in unveiling the mechanisms underlying sex disparities in asthma. In comparison to their female counterparts, male mice are hyperresponsive to inhaled methacholine, a cardinal feature of asthma that contributes to its symptoms. The physiological details and the structural underpinnings of this hyperresponsiveness in males are currently unknown. Herein, BALB/c mice were exposed intranasally to either saline or house dust mite once daily for 10 consecutive days to induce experimental asthma. Twenty-four hours after the last exposure, respiratory mechanics were measured at baseline and after a single dose of inhaled methacholine that was adjusted to trigger the same degree of bronchoconstriction in both sexes (it was twice as high in females). Bronchoalveolar lavages were then collected, and the lungs were processed for histology. House dust mite increased the number of inflammatory cells in bronchoalveolar lavages to the same extent in both sexes (asthma, P = 0.0005; sex, P = 0.96). The methacholine response was also markedly increased by asthma in both sexes (e.g., P = 0.0002 for asthma on the methacholine-induced bronchoconstriction). However, for a well-matched bronchoconstriction between sexes, the increase in hysteresivity, an indicator of airway narrowing heterogeneity, was attenuated in males for both control and asthmatic mice (sex, P = 0.002). The content of airway smooth muscle was not affected by asthma but was greater in males (asthma, P = 0.31; sex, P < 0.0001). These results provide further insights regarding an important sex disparity in mouse models of asthma. The increased amount of airway smooth muscle in males might contribute functionally to their greater methacholine response and, possibly, to their decreased propensity for airway narrowing heterogeneity.

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Section: Discussioncontrasting

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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More airway smooth muscle in males versus females in a mouse model of asthma: A blessing in disguise?

Gill

Rojas‐Ruiz

Boucher

et al. 2023

Experimental Physiology

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“…This can be epitomized by comparing elastance and volume between the lung of humans and mice. The elastance of a human lung (~ 0.005 cmH 2 O/mL), is about 3.7 logs inferior to a mouse lung (~ 30 cmH 2 O/mL) 8 , 15 , mainly because it is also about 3.7 logs bigger (6000 vs. 1 mL).…”

Section: Introductionmentioning

confidence: 91%

“…Interestingly, the elastance of the respiratory system is markedly greater in C57BL/6 than BALB/c mice 8 – 14 . The increased elastance of C57BL/6 versus BALB/c mice is also observed in open-chest conditions ( i.e.…”

Section: Introductionmentioning

confidence: 99%

Lung stiffness of C57BL/6 versus BALB/c mice

Rojas-Ruiz,

Boucher,

Gill

et al. 2023

Sci Rep

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This study was undertaken to determine whether a smaller lung volume or a stiffer lung tissue accounts for the greater lung elastance of C57BL/6 than BALB/c mice. The mechanical properties of the respiratory system and lung volumes were measured with the flexiVent and compared between male C57BL/6 and BALB/c mice (n = 9). The size of the excised lung was also measured by volume liquid displacement. One lobe was then subjected to sinusoidal strains in vitro to directly assess the mechanical properties of the lung tissue, and another one was used to quantify the content of hydroxyproline. In vivo elastance was markedly greater in C57BL/6 than BALB/c mice based on 5 different readouts. For example, respiratory system elastance was 24.5 ± 1.7 vs. 21.5 ± 2.4 cmH2O/mL in C57BL/6 and BALB/c mice, respectively (p = 0.007). This was not due to a different lung volume measured by displaced liquid volume. On the isolated lobes, both elastance and the hydroxyproline content were significantly greater in C57BL/6 than BALB/c mice. These results suggest that the lung elastance of C57BL/6 mice is greater than BALB/c mice not because of a smaller lung volume but because of a stiffer lung tissue due to a greater content of collagen.

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Lung Volumes in a Mouse Model of Pulmonary Allergic Inflammation

Rojas-Ruiz,

Boucher,

Henry

et al. 2024

Lung

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Purpose Air trapping, often attested in humans by elevated residual volume (RV) and ratio of RV on total lung capacity (RV/TLC), is frequently observed in asthma. Confirming these alterations in experimental asthma would be important for translational purposes. Herein, lung volumes were investigated in a mouse model of pulmonary allergic inflammation. Methods Eight- to 10-week-old male C57BL/6 and BALB/c mice were exposed once daily to intranasal house dust mite (HDM) for 10 consecutive days. All readouts were measured 24 h after the last exposure. Lung volumes were assessed with the flexiVent using a new automated method consisting of degassing the lungs followed by a full-range pressure–volume maneuver. The weight and the volume of the lungs were also measured ex vivo and a lobe was further processed for histological analyses. Results HDM exposure led to tissue infiltration with inflammatory cells, goblet cell hyperplasia, thickening of the airway epithelium, and elevated ex vivo lung weight and volume. It also decreased TLC and vital capacity but without affecting RV and RV/TLC. These observations were similar between the two mouse strains. Conclusion Alterations of lung volumes in a murine model of pulmonary allergic inflammation are inconsistent with observations made in human asthma. These discrepancies reflect the different means whereby lung volumes are measured between species. The invasive method used herein enables RV to be measured more precisely and without the confounding effect of air trapping, suggesting that changes in RV and RV/TLC using this method in mice should be interpreted differently than in humans.

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Double-chamber plethysmography versus oscillometry to detect baseline airflow obstruction in a model of asthma in two mouse strains

Cited by 10 publications

References 49 publications

More airway smooth muscle in males versus females in a mouse model of asthma: A blessing in disguise?

More airway smooth muscle in males versus females in a mouse model of asthma: A blessing in disguise?

Lung stiffness of C57BL/6 versus BALB/c mice

Lung Volumes in a Mouse Model of Pulmonary Allergic Inflammation

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