Double Infection of a Cucurbit Host by Zucchini Yellow Mosaic Virus and Cucumber Mosaic Virus

Fattouh, A Faiza

doi:10.3923/ppj.2003.85.90

Cited by 20 publications

(4 citation statements)

References 22 publications

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“…Strikingly, the reduced CMV genome formula variability we observed here appeared to be independent of (i) the titer of the coinfecting virus-AMV constrained the CMV genome formula to the same space at both high and low AMV titers-and (ii) the effect of the co-infecting virus on CMV titer-PVY constrained the genome formula space of CMV but did not affect CMV titer. The lack of effect of PVY on the CMV titer contradicts previous studies reporting either a synergistic increase in the titer of both viruses (21) or an increased CMV titer (38) in a CMV-Potyviridae mixed infection, suggesting that virus-virus interactions may depend on the specific viral strains used. CMV has an exceptionally large host range (~1,200 species) (39), which increases its chance of encountering other viruses in mixed infections more than specialist multipartite viruses such as FBNSV.…”

Section: Genome Formula Variability Of Cucumber Mosaic Virus Decrease...contrasting

confidence: 86%

Mixed viral infection constrains the genome formula of multipartite cucumber mosaic virus

Boezen,

Vermeulen,

Johnson

et al. 2023

Front. Virol.

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Many plant viruses have a multipartite organization, with multiple genome segments packaged into separate virus particles. The genome formula describes the relative frequencies of all viral genome segments, and previous work suggests rapid changes in these frequencies facilitate virus adaptation. Many studies have reported mixed viral infections in plants, often resulting in strong virus–virus interactions. Here, we tested whether mixed infections with tripartite alfalfa mosaic virus (AMV) and monopartite potato virus Y (PVY) affected the genome formula of the tripartite cucumber mosaic virus (CMV), our experimental model. We found that the CMV titer was reduced in mixed infections with its tripartite Bromoviridae relative AMV and in triple infections with both AMV and PVY, indicating notable virus–virus interactions. The variability of the CMV genome formula was significantly lower in mixed infections (CMV and AMV, CMV and PVY, and CMV and AMV and PVY) than in single infections (CMV only). These observations led to the surprising conclusion that mixed infections with two distinct viruses constrain the CMV genome formula. It remains unclear how common these effects are for different combinations of virus species and strains and what the underlying mechanisms are. We, therefore, extended a simulation model to consider three putative scenarios in which a second virus affected the genome formula. The simulation results also suggested that shifts in the genome formula occur, but may not be widespread due to the required conditions. One scenario modeled—co-infection exclusion through niche differentiation—was congruent with the experimental data, as this scenario led to reductions in genome formula variability and titer of the multipartite virus. Whereas previous studies highlighted host–species effects, our results indicate that the genome formula is also affected by mixed infections, suggesting that there is a broader set of environmental cues that affect the genome formula.

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Section: Genome Formula Variability Of Cucumber Mosaic Virus Decrease...contrasting

confidence: 86%

Mixed viral infection constrains the genome formula of multipartite cucumber mosaic virus

Boezen,

Vermeulen,

Johnson

et al. 2023

Front. Virol.

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“…the co-infecting virus on CMV titer: PVY constrains the genome formula space of CMV, but does not affect CMV titer. The lack of effect of PVY on CMV titer contradicts previous studies reporting either a synergistic increase in titer of both viruses (Mascia et al 2010) or increased CMV titer (Fattouh 2003) in CMV-Potyviridae mixed infection, suggesting the virus-virus interactions may depend on the specific viral strains used. CMV has an exceptionally large host range (~1200 species) (P. Palukaitis and García-Arenal 2019), which increases its chance of encounters with other viruses in mixed infection compared to specialist multipartite viruses such as FBNSV.…”

Section: Genome Formula Variability Of CMV Decreases Under Mixed Infe...contrasting

confidence: 85%

The ecology of multipartite plant viruses : Insight into genome plasticity from experimental investigation and metagenomics

Boezen

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“…Mixed infection of CMV and potyviruses has been studied by many scientists (Wang et al 2002, Fattouh 2003, Mascia et al 2010, Fukuzawa et al 2010, Barbosa et al 2016, Arogundade et al 2018, Salehzadeh 2018). As a rule, researchers reported about synergistic effect on different hosts.…”

Section: Discussionmentioning

confidence: 99%

Cucumber mosaic virus and turnip mosaic virus occurrence in garlic mustard in Ukraine

Kyrychenko¹,

Snihur²,

Shevchenko³

et al. 2023

Biol. plant.

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Garlic mustard (Alliaria petiolata) is an herbaceous biennial plant native to Europe. In Ukraine, in addition to becoming a serious invader, garlic mustard can serve as a host to several viruses, which may affect agricultural crops. In view of this, the purpose of the study was to identify the virome of garlic mustard growing in Ukraine. Plant samples collected in Kyiv regions were tested for the presence of cucumber mosaic virus (CMV), turnip mosaic virus (TuMV), turnip yellow mosaic virus (TYMV), watermelon mosaic virus II (WMV), and turnip crinkle virus (TCV) by serological and/or molecular methods. According to the results found in the present study, symptomatic A. petiolata obtained in 2021 were infected with CMV (60%), TuMV (20%), or co-infected with CMV + TuMV (20%). TYMV, WMV II, and TCV were not detected in any of the collected samples. The cDNA fragments encoded the coat protein (CP) gene of CMV and TuMV were sequenced and named as CMV-Ap and TuMV-Ap, respectively. In phylogenetic analysis, the CMV-Ap closely resembled the German isolate MW582807 (Sarracenia sp.), with 99.8% nucleotide identity and belongs to subgroup II of CMV. In the phylogenetic tree, TuMV-Ap clustered with isolates AP017803, AP017764, AP017791, and JQ073722, and represented the highest identity (98.6%) to Iranian isolate IRNTRa9 (AP017803) from Rapistrum rugosum and Turkish isolate TUR49 (AP017872) from Raphanus raphanistrum. The sequences of CMV-Ap and TuMV-Ap were deposited in the GenBank under Accession Numbers MZ540213 and OM799323, respectively. The results obtained in the study indicate the important role of infected garlic mustard as alternative host and natural reservoir of CMV and TuMV from which these economically important viruses can spread to other wild and cultivated plants. This is the first molecular evidence of TuMV infection in A. petiolata from Ukraine.

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Double Infection of a Cucurbit Host by Zucchini Yellow Mosaic Virus and Cucumber Mosaic Virus

Cited by 20 publications

References 22 publications

Mixed viral infection constrains the genome formula of multipartite cucumber mosaic virus

Mixed viral infection constrains the genome formula of multipartite cucumber mosaic virus

The ecology of multipartite plant viruses : Insight into genome plasticity from experimental investigation and metagenomics

Cucumber mosaic virus and turnip mosaic virus occurrence in garlic mustard in Ukraine

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