Double mutation of BRF1 and BRF2 leads to sterility in Arabidopsis thaliana

Zhang, Kaiyue; Yang, Wenwen; Yu, Hongbin; Fu, Can; Liu, Xiaxia; Liu, Jian

doi:10.1016/j.bbrc.2019.06.138

Cited by 6 publications

(9 citation statements)

References 28 publications

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“…To overcome the functional redundancy among the three TFIIB-like homologs, double mutants were also generated through genetic crosses. The resulting double mutants for AtBRF1 and AtBRF3 or for AtBRF2 and AtBRF3 were normal in both growth and reproduction [28]. By contrast, no double mutant for AtBRF1 and AtBRF2 was obtained, suggesting that a double mutation of AtBRF1 and AtBRF2 results in the infertility of Arabidopsis [28].…”

Section: Atbrf1-3mentioning

confidence: 92%

“…AtBRF1, 2, and 3 are three closely related TFIIB-like factors highly homologous to the yeast and human BRF TFIIB-related Pol III GTFs. Expression analysis using the GUS reporter gene showed that the expression of AtBRF1 and AtBRF2 was mainly detected in the anthers and ovary [28]. In comparison, expression of AtBRF2 was stronger than that of AtBRF1, but expression of AtBRF3 was hardly detected [28].…”

Section: Atbrf1-3mentioning

confidence: 97%

“…Expression analysis using the GUS reporter gene showed that the expression of AtBRF1 and AtBRF2 was mainly detected in the anthers and ovary [28]. In comparison, expression of AtBRF2 was stronger than that of AtBRF1, but expression of AtBRF3 was hardly detected [28]. qRT-PCR analysis further showed that in three-week-old seedlings grown under normal conditions the expression of AtBRF2 was 0.7 times higher than that of AtBRF1, and was 1.6 times higher than that of AtBRF3.…”

Section: Atbrf1-3mentioning

confidence: 99%

“…qRT-PCR analysis further showed that in three-week-old seedlings grown under normal conditions the expression of AtBRF2 was 0.7 times higher than that of AtBRF1, and was 1.6 times higher than that of AtBRF3. To directly address the biological functions of the three TFIIB-like factors, T-DNA knockout mutants were isolated but found to be normal in both growth and development [28]. To overcome the functional redundancy among the three TFIIB-like homologs, double mutants were also generated through genetic crosses.…”

Section: Atbrf1-3mentioning

confidence: 99%

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Expansion and Functional Diversification of TFIIB-Like Factors in Plants

Yang

Fan

et al. 2021

IJMS

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As sessile organisms, plants have evolved unique patterns of growth and development, elaborate metabolism and special perception and signaling mechanisms to environmental cues. Likewise, plants have complex and highly special programs for transcriptional control of gene expression. A case study for the special transcription control in plants is the expansion of general transcription factors, particularly the family of Transcription Factor IIB (TFIIB)-like factors with 15 members in Arabidopsis. For more than a decade, molecular and genetic analysis has revealed important functions of these TFIIB-like factors in specific biological processes including gametogenesis, pollen tube growth guidance, embryogenesis, endosperm development, and plant-microbe interactions. The redundant, specialized, and diversified roles of these TFIIB-like factors challenge the traditional definition of general transcription factors established in other eukaryotes. In this review, we discuss general transcription factors in plants with a focus on the expansion and functional analysis of plant TFIIB-like proteins to highlight unique aspects of plant transcription programs that can be highly valuable for understanding the molecular basis of plant growth, development and responses to stress conditions.

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Section: Atbrf1-3mentioning

confidence: 92%

Section: Atbrf1-3mentioning

confidence: 97%

Section: Atbrf1-3mentioning

confidence: 99%

Section: Atbrf1-3mentioning

confidence: 99%

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Expansion and Functional Diversification of TFIIB-Like Factors in Plants

Yang

Fan

et al. 2021

IJMS

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“…We further bred the double mutants of BRFs. The homogenous double mutants of brf1 brf3 and brf2 brf3 were generated, while the double mutation of BRF1 and BRF2 led to sterility (Zhang et al, 2019). We determined the thermotolerance of brf1 brf3 and brf2 brf3.…”

Section: The Fes1a Mutants Exhibit Differential Sensitivities To Heatmentioning

confidence: 99%

BRF Negatively Regulates Thermotolerance Defect of fes1a in Arabidopsis

Liu

et al. 2020

Front. Plant Sci.

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FES1A is a heat shock protein 70 binding protein. Mutation of FES1A leads to a defect in thermotolerance of Arabidopsis; however, independent fes1a mutants exhibit a range in the extent of thermosensitivity. Here, we found that BRF2, a gene adjacent to FES1A and encoding a component of transcription factor IIIB, affects the thermosensitivity of fes1a mutants. Knockout of BRF2 suppressed fes1a thermosensitivity, while overexpression of BRF2 increased thermosensitivity of fes1a. BRF2 in fes1a mutants regulates the transcriptional strength of RNA Polymerase II and accumulation of heat shock proteins and eventually affects the thermotolerance of fes1a. There is a cross-talking between RNA Pol III and Pol II. The cross-talking is initiated by BRF, magnified by the mutation of FES1A, and finally has an effect on thermotolerance.

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