Double-ratiometric fluorescence imaging of H2Se and O2˙− under hypoxia for exploring Na2SeO3-induced HepG2 cells' apoptosis

Gao, Xiaonan; Guo, Wenfei; Ge, Lihong; Kong, Fanming; Xu, Kehua; Tang, Bo

doi:10.1039/c8ra08142e

“…Using two fluorescent probes for hydrogen selenide (H 2 Se) and O 2 .− as well as the reference fluorophore Rhodamine 110, our group realized simultaneous fluorescence imaging of H 2 Se and O 2 .− with a double‐ratiometric method . It was found that the H 2 Se concentration obviously increased under sodium selenite (Na 2 SeO 3 )‐induced HepG2 cell apoptosis, but the O 2 .− level showed almost no change.…”

Section: Current Status Of Fluorescent Probes For O2−mentioning

confidence: 99%

Versatile Fluorescent Probes for Imaging the Superoxide Anion in Living Cells and In Vivo

Xiao

¹

,

Zhang

²

,

Li

³

et al. 2019

Self Cite

View full text Add to dashboard Cite

The superoxide anion (O2.−) is widely engaged in the regulation of cell functions and is thereby intimately associated with the onset and progression of many diseases. To ascertain the pathological roles of O2.− in related diseases, developing effective methods for monitoring O2.− in biological systems is essential. Fluorescence imaging is a powerful tool for monitoring bioactive molecules in cells and in vivo owing to its high sensitivity and high temporal‐spatial resolution. Therefore, increasing numbers of fluorescent imaging probes have been constructed to monitor O2.− inside live cells and small animals. In this minireview, we summarize the methods for design and application of O2.−‐responsive fluorescent probes. Moreover, we present the challenges for detecting O2.− and suggestions for constructing new fluorescent probes that can indicate the production sites and concentration changes in O2.− as well as O2.−‐associated active molecules in living cells and in vivo.

show abstract

“…Measurements were made under hypoxic conditions (vs. normoxic conditions). In the study of anticancer selenium–containing compounds, the biological conclusion ascertained by this synergetic fluorophore study was that Na 2 SeO 3 induces cell apoptosis which is achieved under reducing and not oxidizing conditions [ 102 ].…”

Section: Analytes Containing Seleniummentioning

confidence: 99%

“…study was that Na2SeO3 induces cell apoptosis which is achieved under reducing and not oxidizing conditions [102]. 60).…”

Section: Reduced Organic Selenium (Selenocysteine) (Biological)) Eg H2sementioning

confidence: 99%

Discussions of Fluorescence in Selenium Chemistry: Recently Reported Probes, Particles, and a Clearer Biological Knowledge

Abdillah

¹

,

Sonawane

²

,

Kim

³

et al. 2021

View full text Add to dashboard Cite

In this review from literature appearing over about the past 5 years, we focus on selected selenide reports and related chemistry; we aimed for a digestible, relevant, review intended to be usefully interconnected within the realm of fluorescence and selenium chemistry. Tellurium is mentioned where relevant. Topics include selenium in physics and surfaces, nanoscience, sensing and fluorescence, quantum dots and nanoparticles, Au and oxide nanoparticles quantum dot based, coatings and catalyst poisons, thin film, and aspects of solar energy conversion. Chemosensing is covered, whether small molecule or nanoparticle based, relating to metal ion analytes, H2S, as well as analyte sulfane (biothiols—including glutathione). We cover recent reports of probing and fluorescence when they deal with redox biology aspects. Selenium in therapeutics, medicinal chemistry and skeleton cores is covered. Selenium serves as a constituent for some small molecule sensors and probes. Typically, the selenium is part of the reactive, or active site of the probe; in other cases, it is featured as the analyte, either as a reduced or oxidized form of selenium. Free radicals and ROS are also mentioned; aggregation strategies are treated in some places. Also, the relationship between reduced selenium and oxidized selenium is developed.

show abstract

Versatile Fluorescent Probes for Imaging the Superoxide Anion in Living Cells and In Vivo

Xiao¹,

Zhang²,

Li³

et al. 2019

Self Cite

View full text Add to dashboard Cite

The superoxide anion (O2.−) is widely engaged in the regulation of cell functions and is thereby intimately associated with the onset and progression of many diseases. To ascertain the pathological roles of O2.− in related diseases, developing effective methods for monitoring O2.− in biological systems is essential. Fluorescence imaging is a powerful tool for monitoring bioactive molecules in cells and in vivo owing to its high sensitivity and high temporal‐spatial resolution. Therefore, increasing numbers of fluorescent imaging probes have been constructed to monitor O2.− inside live cells and small animals. In this minireview, we summarize the methods for design and application of O2.−‐responsive fluorescent probes. Moreover, we present the challenges for detecting O2.− and suggestions for constructing new fluorescent probes that can indicate the production sites and concentration changes in O2.− as well as O2.−‐associated active molecules in living cells and in vivo.

show abstract

Double-ratiometric fluorescence imaging of H₂Se and O₂˙⁻ under hypoxia for exploring Na₂SeO₃-induced HepG2 cells' apoptosis

Abstract: Sodium selenite (Na2SeO3), as an anti-tumor drug for inducing tumor cells' apoptosis, has been widely studied under normoxic conditions and has been shown to exhibit oxidative stress process-induced apoptosis.

Cited by 8 publications

References 41 publications

Versatile Fluorescent Probes for Imaging the Superoxide Anion in Living Cells and In Vivo

Versatile Fluorescent Probes for Imaging the Superoxide Anion in Living Cells and In Vivo

Discussions of Fluorescence in Selenium Chemistry: Recently Reported Probes, Particles, and a Clearer Biological Knowledge

Versatile Fluorescent Probes for Imaging the Superoxide Anion in Living Cells and In Vivo

Contact Info

Product

Resources

About