Double‐Strand Breaks in Genome‐Sized DNA Caused by Ultrasound

Abstract

DNA double‐strand breaks (DSBs) caused by ultrasound were evaluated in a quantitative manner by single‐molecule fluorescence microscopy. We compared the effect of time‐interval (or pulse) sonication to that of continuous wave (CW) sonication at a fixed frequency of 30 kHz. Pulses caused fewer DSBs than CW sonication under the same total input ultrasound energy when the pulse repetition period was above the order of a second. In contrast, pulses caused more DSBs than CW sonication for pulse widths shorter than a second. These effect of ultrasound on DNA were interpreted in terms of the time‐dependent decay in the probability of breakage during the duration of a pulse. We propose a simple phenomenological model by considering a characteristic decay in the probability of DSBs during single‐pulse sonication, which reproduces the essence of the experimental trend. In addition, a data analysis revealed a characteristic scaling behavior between the number of pulses and the number of DSBs.

Image processing method for automatic measurement of number of DNA breaks

“…Many research reports that DNA is damaged by chemical and physical reactions in environmental condition. For example, ultrasonic which is important for application in practical medicine causes double-strand breaks in genome-sized DNA [1]. Photo induced Journal of Advanced Simulation in Science and Engineering damages in DNA are also investigated [2].…”

Vibration Characteristics and Persistence of Poloxamer- or Phospholipid-Coated Single Microbubbles under Ultrasound Irradiation

“…When used for delivery, collapse of the microbubbles can be initiated by ultrasound irradiation, which induces microjets and provokes the release of the drug or gene to cells. The effects of bubble collapse on tissues and their safety have been investigated. − Yoshikawa’s group reported the effects of ultrasound on deoxyribonucleic acid (DNA) and measured the breaking of doubled-strand DNA under sonication at low ultrasound frequency. , Cavitation of microbubbles induced by high-intensity ultrasound increases local temperature and can enhance therapeutic effects − …”

“…The effects of bubble collapse on tissues and their safety have been investigated [16][17][18][19]. Yoshikawa's group reported the effects of ultrasound on deoxyribonucleic acid (DNA) and measured the breaking of doubled-strand DNA under sonication at low ultrasound frequency [20,21]. Cavitation of microbubbles induced by high-intensity ultrasound increases local temperature and can enhance therapeutic effects [22][23][24][25].…”

Ultrasonic Bending of Silver Nanowires

“…Ultrasonic cavitation has thermal and mechanical effects . The thermal effect refers to the spillover of the local high temperature, which can be used to promote chemical reactions, such as inducing reactive oxygen species .…”

“…1,9 Ultrasonic cavitation has thermal and mechanical effects. 10 The thermal effect refers to the spillover of the local high temperature, which can be used to promote chemical reactions, such as inducing reactive oxygen species. 6 The mechanical effect refers to the broader effects of shear stress and shock waves, which can be used for emulsification, tissue cutting, and skin penetration.…”