Down-modulation of heat shock protein 70 and up-modulation of Caspase-3 during schisandrin B-induced apoptosis in human hepatoma SMMC-7721 cells

Abstract

Sch B is able to inhibit the proliferation of human hepatoma SMMC-7721 cells and induce apoptosis, which goes through Caspase-3-dependent and Caspase-9-independent pathway accompanied with the down-regulation of Hsp70 protein expression at an early event.

Schisandrin A and B affect subventricular zone neurogenesis in mouse

“…Three doses of Sch A and B (1, 10 and 20 mg/kg d) were chosen in this study, which were much lower than the most tolerated or toxic dosage (Hu et al, 2012a;Ko and Lam, 2002;Ko et al, 2008). It was reported that Sch B acts as a hormetic agent in cultured cells, with cytoprotective effects predominating at low concentrations and cytotoxicity occurring at high concentrations (Wu et al, 2004;Chiu et al, 2006). Therefore, to determine an appropriate dosage of Sch A and B for subsequent analysis, we preliminarily analyzed the samples by immunofluorescent staining in the SVZ, RMS and OB.…”

Activity of Schisandrin C Isolated fromSchisandra chinensisagainst Human Cancer Cell Lines

“…Schisandrin B induced apoptosis through caspase-3-dependent pathways accompanied with the down-regulation of heat shock protein 70 protein expression at an early event in human hepatoma SMMC-7721 cells (Wu et al, 2004). Schisandrin B can also substantially enhance caspase-9 accompanied with a significant elevation of mitochondrial breakdown in SMMC-7721 cells (Li et al, 2006).…”

“…Therefore, the development of novel potent, but non-toxic anti-cancer reagents is worth a continuous effort. In the last decade, dibenzocyclooctadiene lignans (e.g., deoxyschisandrin, γ -schisandrin, schisandrin C, schisandrin, gomisin A, schisantherin A) (He et al, 1997) has attracted considerable attention as a potential anti-cancer drug or an adjuvant to the conventional therapeutic modalities for human hepatocellular cancer (Wu et al, 2004;Pan et al, 2006;Li et al, 2007). The present study was undertaken to assess the antitumor activity of schisandrin C against three established human cancer cell lines.…”

“…In clinical situations it has been used for the treatment of patients suffering from viral and chemical hepatitis since the 1970s (Hancke et al, 1999). Analysis of the chemical composition of Schisandra chinensis demonstrated that the biologically active compounds are dibenzocyclooctadiene lignans, which have revealed a wide variety of active effects including antihepatotoxic (Chiu et al, 2003;Ip et al, 2000;Hikino et al, 1984), antioxidant and detoxificant (Ip et al, 1995;Ip & Ko, 1996;Mak et al, 1997;Chiu et al, 2005), anticancer (Wu et al, 2004), and anti-amnestic activities (Kim et al, , 2006. Recently, dibenzocyclooctadiene lignans were reported to reverse cancer drug resistance by targeting P-glycoprotein (P-gp) (Pan et al, 2005(Pan et al, , 2006Wan et al 2006), multidrug resistance-associated protein 1 (MRP1) (Li et al, 2007;Sun et al, 2007), and enhancing apoptosis (Li et al, 2006).…”

Inhibitory effect of schisandrin B on gastric cancer cells in vitro

“…For example, schisandrin B can inhibit the proliferation of human hepatoma SMMC-7721 cells and induce apoptosis, involving the caspase-3-dependent and caspase-9-independent pathways, accompanied by downregulation of Hsp70 protein expression at an early stage [2] . Schisandrin B enhances doxorubicin-induced apoptosis of SMMC7721 cells and MCF-7, a human breast cancer cell line, but not normal cells.…”