Down-regulation of Cdk1 activity in G1 coordinates the G1/S gene expression programme with genome replication

García-Blanco, Natalia; Moreno, Sergio

doi:10.1007/s00294-018-00926-y

Cited by 11 publications

(6 citation statements)

References 73 publications

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“…In S. pombe , TORC2 has been implicated in the regulation of nutrient-dependent and stress-induced gene transcription, including the regulation of amino acids permeases [ 150 ], nitrogen-starvation [ 151 ] and DNA-replication stress-induced genes [ 85 ]. The MBF (Mlu-Binding Factor) complex is required for cell-cycle regulated induction of G1/S transition and DNA replication genes and it is activated by Cdk1 at the end of G1 and in response to replication stress [ 152 ]. Loss of Sp TORC2, or its target kinase Gad8, resulted in a decrease in the binding efficiency of the MBF complex to its cognate promoters and loss of MBF-dependent gene induction in response to DNA replication stress [ 85 ].…”

Section: Cellular Roles Of Tor In Transcription and Epigenetic Regmentioning

confidence: 99%

Nuclear Functions of TOR: Impact on Transcription and the Epigenome

Laribee

Weisman

2020

Genes

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The target of rapamycin (TOR) protein kinase is at the core of growth factor- and nutrient-dependent signaling pathways that are well-known for their regulation of metabolism, growth, and proliferation. However, TOR is also involved in the regulation of gene expression, genomic and epigenomic stability. TOR affects nuclear functions indirectly through its activity in the cytoplasm, but also directly through active nuclear TOR pools. The mechanisms by which TOR regulates its nuclear functions are less well-understood compared with its cytoplasmic activities. TOR is an important pharmacological target for several diseases, including cancer, metabolic and neurological disorders. Thus, studies of the nuclear functions of TOR are important for our understanding of basic biological processes, as well as for clinical implications.

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Section: Cellular Roles Of Tor In Transcription and Epigenetic Regmentioning

confidence: 99%

Nuclear Functions of TOR: Impact on Transcription and the Epigenome

Laribee

Weisman

2020

Genes

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“… 15 - 17 Evidence has shown the inhibitory effect of miR-143 works through pathway G1-S transition, 18 with cyclin-dependent kinase 1 (CDK1) a regulatory factor in the process of cell cycle. 19 …”

Section: Introductionmentioning

confidence: 99%

“…[15][16][17] Evidence has shown the inhibitory effect of miR-143 works through pathway G1-S transition, 18 with cyclin-dependent kinase 1 (CDK1) a regulatory factor in the process of cell cycle. 19 TMPO-AS1 promotes the proliferation and viability of estrogen receptor (ER)-positive breast cancer cells in vitro and in vivo. 20 Besides, little research has been done on TMPO-AS1, not to mention its potential effect in LC.…”

Section: Introductionmentioning

confidence: 99%

Down-Regulation of TMPO-AS1 Induces Apoptosis in Lung Carcinoma Cells by Regulating miR-143-3p/CDK1 Axis

Qiu

Bian

2021

Technol Cancer Res Treat

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Evidence has shown that long non-coding RNAs (lncRNA) play pivotal roles in cancer promotion as well as suppression. But the molecular mechanism of lncRNA TMPO antisense transcript 1 (TMPO-AS1) in lung cancer (LC) remains unclear. This study mainly investigated the effect of TMPO-AS1 in LC treatment. TMPO-AS1 was tested by Kaplan-Meier method. Quantitative real time polymerase chain reaction (qRT-PCR) was employed to assess the expressions of TMPO-AS1, miR-143-3p, and CDK1 respectively in LC tissues and cell lines. TMPO-AS1, miR-143-3p and CDK1 expressions in LC cells were regulated through cell transfection, followed by MTT for cell viability detection. Besides, dual-luciferase reporter assays were performed to verify the interrelated microRNA of TMPO-AS1 and the target of miR-143-3p. Western blot experiments were used to examine the expressions of apoptosis-related proteins, and flow cytometry tested the cell apoptosis in treated cells. TMPO-AS1 and CDK1 were overexpressed in LC tissues and cells, while miR-143-3p level was suppressed. The decrease of TMPO-AS1 led to the increase of miR-143-3p, which further resulted in the reduction of CDK1. Down-regulating TMPO-AS1 reduced LC cell viability, motivated cell apoptosis, as well as promoted the expressions of Bcl and CCND1 and restrained Caspase-3 level, but all these consequences were abrogated by miR-143-3p inhibitor. Simultaneously, siCDK1 could reverse the anti-apoptosis and pro-activity functions of miR-143-3p inhibitor in LC cells. Down-regulation of TMPO-AS1 has the effects of pro-apoptosis in LC by manipulating miR-143-3p/CDK1, which is hopeful to be a novel therapy for LC patients.

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“…In small mutant cells (like wee1 ) lacking size control in G2, the otherwise cryptic G1 sizer becomes active to maintain homeostasis (Fantes & Nurse, 1981). In this case, a specific transcription program ensures the operation of this mechanism, which requires an elongated G1 phase to be stabilized (García‐Blanco & Moreno, 2019).…”

Section: Introductionmentioning

confidence: 99%

Strongly oversized fission yeast cells lack any size control and tend to grow linearly rather than bilinearly

Nagy

Medgyes-Horváth

Vörös

et al. 2020

Yeast

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During the mitotic cycle, the rod‐shaped fission yeast cells grow only at their tips. The newly born cells grow first unipolarly at their old end, but later in the cycle, the ‘new end take‐off’ event occurs, resulting in bipolar growth. Photographs were taken of several steady‐state and induction synchronous cultures of different cell cycle mutants of fission yeast, generally larger than wild type. Length measurements of many individual cells were performed from birth to division. For all the measured growth patterns, three different functions (linear, bilinear and exponential) were fitted, and the most adequate one was chosen by using specific statistical criteria, considering the altering parameter numbers. Although the growth patterns were heterogeneous in all the cultures studied, we could find some tendencies. In cultures with sufficiently wide size distribution, cells large enough at birth tend to grow linearly, whereas the other cells generally tend to grow bilinearly. We have found that among bilinearly growing cells, the larger they are at birth, the rate change point during their bilinear pattern occurs earlier in the cycle. This shifting near to the beginning of the cycle might finally cause a linear pattern, if the cells are even larger. In all of the steady‐state cultures studied, a size control mechanism operates to maintain homeostasis. By contrast, strongly oversized cells of induction synchronous cultures lack any sizer, and their cycle rather behaves like an adder. We could determine the critical cell size for both the G1 and G2 size controls, where these mechanisms become cryptic. TAKE AWAY Most individual fission yeast cells in steady‐state cultures grow bilinearly. In strongly oversized fission yeast cells, linear growth dominates over bilinear. Above birth length thresholds, both the G1 and G2 size controls become cryptic.

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Down-regulation of Cdk1 activity in G1 coordinates the G1/S gene expression programme with genome replication

Cited by 11 publications

References 73 publications

Nuclear Functions of TOR: Impact on Transcription and the Epigenome

Nuclear Functions of TOR: Impact on Transcription and the Epigenome

Down-Regulation of TMPO-AS1 Induces Apoptosis in Lung Carcinoma Cells by Regulating miR-143-3p/CDK1 Axis

Strongly oversized fission yeast cells lack any size control and tend to grow linearly rather than bilinearly

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