Down-Regulation of Glycosylphosphatidylinositol-Specific Phospholipase D Induced by Lipopolysaccharide and Oxidative Stress in the Murine Monocyte- Macrophage Cell Line RAW 264.7

Du, Xiaohan; Low, Martin G.

doi:10.1128/iai.69.5.3214-3223.2001

Cited by 22 publications

(17 citation statements)

References 49 publications

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“…[12] In fact, macrophages generate superoxide in vitro through a PLD1-dependent mechanism. [2,5] These phenomena are doubtless the same in the macrophages present in EAN lesions, since we have shown here that perivascular macrophages express PLD1, which may in turn be linked to the generation of free radicals (including nitric oxide). The roles of macrophages in EAN are both beneficial and detrimental, because …”

Section: Shin Et Almentioning

confidence: 81%

Increased Expression of Phospholipase D1 in the Sciatic Nerve of Rats With Experimental Autoimmune Neuritis

Shin

Min

Ahn

et al. 2002

Immunological Investigations

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Phospholipase D1 (PLD1) expression in the sciatic nerve was studied in induced experimental autoimmune neuritis (EAN) in Lewis rats. PLD1 immunoreactivity was seen in some Schwann cells in the sciatic nerves of normal rats. In parallel with the progression of EAN, PLD1-positive Schwann cells significantly increased in number and showed intense immunoreactivity. PLD1 was also detected in some ED1+ macrophages in EAN lesions. These results suggest that PLD1 in macrophages and Schwann cells plays an important role in the activation of these cells in the pathogenesis of EAN, an animal model of human peripheral demyelinating disease.

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Section: Shin Et Almentioning

confidence: 81%

Increased Expression of Phospholipase D1 in the Sciatic Nerve of Rats With Experimental Autoimmune Neuritis

Shin

Min

Ahn

et al. 2002

Immunological Investigations

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“…CD14 has two distinct forms: mCD14 (GPI-anchored form) and sCD14 (soluble CD14). mCD14 is believed to directly affect LPS simulation via interaction with Toll-like receptor 4 [35] . Interferon gamma (IFNγ) induces iNOS via the JAK-STAT signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of iNOS Expression in Esophageal Cancer Patients

Barani

Motalleb

Maghsoudi

2016

Gastrointest Tumors

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Background: Esophageal cancer is a public health concern around the world; this cancer is the sixth leading cause of death of cancer in the world with about 386,000 deaths per year. Its risk factors include environmental factors such as tobacco smoke, gastroesophageal reflux and genetic changes. iNOS is stated by the effect of various inflammatory factors and is thus called inducible NOS. Investigating iNOS expression is a powerful tool for understanding effective molecular parameters at tissue and cellular responses to external factors. In this research work, iNOS expression in patients with esophageal cancer was studied in Iran. Materials and Methods: 15 formalin-fixed and paraffin-embedded (FFPE) esophageal cancer tissue samples and 15 normal FFPE samples were collected from various medical centers (Zabol, Zahedan, Kashan) to measure iNOS expression by real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR). All PCR reactions were conducted by three replicates for iNOS and internal control (β-actin) by 2 -ΔΔCT (Livak) method. Differences were measured in target gene expression in patients and control group using the t test. All statistical analyses were done using the SPSS software. Results: The results showed that there was no significant difference between iNOS expression in the case and control groups (p > 0.05); however, there was an increase in iNOS expression in the case group. On the other hand, there was a significant difference between iNOS expression in males and females in the two groups of healthy subjects and patients, and it was higher in women than in men. Conclusion: Further studies need to be conducted with larger sample sizes and in other populations to validate these findings.

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“…In the present study, PLD1 immunoreactivity was intense in the inflammatory cells of EAU-affected retinas, particularly within macrophages. Indeed, macrophages have been shown to generate superoxide in vitro via a PLD1-dependent mechanism (Du and Low, 2001). This suggests that the macrophages that express PLD1 in the retina of rats with EAU have been activated.…”

Section: Discussionmentioning

confidence: 99%

Phospholipase D1 Is Up-Regulated in the Retina of Lewis Rats with Experimental Autoimmune Uveoretinitis

Kang¹,

Ahn²,

Moon³

et al. 2005

LIMM

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To investigate whether phospholipase D1 (PLD1) is involved in autoimmune damage to the eyes, we used Western blotting and immunohistochemistry to examine the expression and distribution of PLD1 in the retinas of Lewis rats with experimental autoimmune uveoretinitis (EAU). Western blot analysis showed that the level of expression of PLD1 was significantly increased in EAU-affected retinas compared to that of control. Immunohistochemical analysis showed that ganglion cells and Muller cells, which express PLD1 weakly in the normal retina, showed increased expression of PLD1 in EAU-affected retinas; some ED1-immunopositive cells were also immunopositive for PLD1 in lesions at post-immunization days 14 and 21. These results suggest that the increased expression of PLD1 in inflammatory cells exacerbates an autoimmune response in EAU, while its expression in ganglion cells limits cell loss by activation of survival factors in eyes with autoimmune injury.

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Down-Regulation of Glycosylphosphatidylinositol-Specific Phospholipase D Induced by Lipopolysaccharide and Oxidative Stress in the Murine Monocyte- Macrophage Cell Line RAW 264.7

Cited by 22 publications

References 49 publications

Increased Expression of Phospholipase D1 in the Sciatic Nerve of Rats With Experimental Autoimmune Neuritis

Increased Expression of Phospholipase D1 in the Sciatic Nerve of Rats With Experimental Autoimmune Neuritis

Evaluation of iNOS Expression in Esophageal Cancer Patients

Phospholipase D1 Is Up-Regulated in the Retina of Lewis Rats with Experimental Autoimmune Uveoretinitis

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