Down-regulation of miR-605 promotes the proliferation and invasion of prostate cancer cells by up-regulating EN2

Zhou, Yuanyuan; Yang, Haonan; Xia, Wei; Cui, Li; Xu, Ran; Lü, Hao; Zhou, Xue; Zhang, Bo; Tian, Zinong; Cao, Yunjie; Zhou, Xing; Yin, Shuai; He, Xiaozhou

doi:10.1016/j.lfs.2017.09.028

Cited by 31 publications

(31 citation statements)

References 22 publications

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“…Cell migration and invasion was assayed according to the methods described previously [25,26]. After treatment with PSH for 24 h, the surviving SCC-9, SAS and HSC-3 cells were harvested and seeded to upper Transwell (Greiner Bio-One, North Carolina, USA), and DMEM-F12 medium supplemented with 10% FBS (600 µl) was added in advance into Transwell lower chambers in a 24-well plate, then incubated for 24 h at 37°C.…”

Section: Cell Migration and Invasion Assaysmentioning

confidence: 99%

Pinostilbene Hydrate Suppresses Human Oral Cancer Cell Metastasis by Downregulation of Matrix Metalloproteinase-2 Through the Mitogen-Activated Protein Kinase Signaling Pathway

Hsieh

Chin

Lin

et al. 2018

Cell Physiol Biochem

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Background/Aims: Cancer is the most common cause of death worldwide with approximately one third of people being diagnosed with cancer in their lifetime. Pinostilbene hydrate (PSH) A methylated derivative of resveratrol Has been reported to possess antioxidative Cardioprotective and anticancer properties. However the antimetastatic effect of pinostilbene in oral squamous cell carcinoma (OSCC) remains unknown. Methods: In this study We investigated the effect of PSH on antimetastatic activity and the relevant signaling pathways underlying mechanisms of SCC-9 SAS and HSC-3 oral cancer cell lines by MTT assay Wound healing Transwell assay Zymography and western blot analysis. Results: Our findings indicated that PSH inhibits migration and invasion ability by reducing the protein activity and expression of matrix metalloproteinases-2 (MMP-2) in all three cell lines. Moreover • The phosphorylation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinases (p38) had significant inhibitory effects in the presence of PSH in the SCC9 and SAS cell lines. A combination of ERK1/2 and p38 inhibitors with PSH also reduced the migration and activity of MMP-2 in the SCC9 and SAS cell lines. Conclusion: This study demonstrated that PSH suppresses MMP-2 enzymatic activity by downregulating the p38/ERK1/2 pathway and that it might be a promising agent for preventing OSCC cell metastasis.

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Section: Cell Migration and Invasion Assaysmentioning

confidence: 99%

Pinostilbene Hydrate Suppresses Human Oral Cancer Cell Metastasis by Downregulation of Matrix Metalloproteinase-2 Through the Mitogen-Activated Protein Kinase Signaling Pathway

Hsieh

Chin

Lin

et al. 2018

Cell Physiol Biochem

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“…Zhou et al (2017) demonstrated that engrailed homeobox 2 (EN2) is inversely modulated through miR-605, and miR-605 downregulation which stimulates the expansion and invasion of cells from PCa patients by overexpressing EN2, subsequently eventuates in PCa progression.5.8 | miR-940miR-940 is upmodulated in PCa cells and is regarded as the main modulator of migration and invasion enhancer 1 (MIEN1), which is directly targeted by miR-940. It was observed that miR-940 prevented migratory and invasive cells, diminished their anchorageindependent growth and E-cadherin overexpression by involving in MET.…”

mentioning

confidence: 99%

The role of microRNAs in prostate cancer migration, invasion, and metastasis

Aghdam

Ebrazeh

Hemmatzadeh

et al. 2018

Journal Cellular Physiology

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Prostate cancer (PCa) is considered the most prevalent malignancy and the second major cause of cancer‐related death in males from Western countries. PCa exhibits variable clinical pictures, ranging from dormant to highly metastatic cancer. PCa suffers from poor prognosis and diagnosis markers, and novel biomarkers are required to define disease stages and to design appropriate therapeutic approach by considering the possible genomic and epigenomic differences. MicroRNAs (miRNAs) comprise a class of small noncoding RNAs, which have remarkable functions in cell formation, differentiation, and cancer development and contribute in these processes through controlling the expressions of protein‐coding genes by repressing translation or breaking down the messenger RNA in a sequence‐specific method. miRNAs in cancer are able to reflect informative data about the current status of disease and this might benefit PCa prognosis and diagnosis since that is concerned to PCa patients and we intend to highlight it in this paper.

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“…miR-605 also functions as a tumor suppressor in melanoma by regulating the expression of inositol polyphosphate-4-phosphatase type II B ( 35 ). In addition, miR-605 was also demonstrated to affect the proliferation, migration and invasion of non-small cell lung cancer, prostate cancer, and breast cancer ( 18 , 19 , 36 ). In the present study, miR-605 was found to be a vital factor in the progression of OS, as the downregulation of miR-605 significantly promoted cell proliferation, migration, and invasion of OS, which indicates that miR-605 may be involved in the progression and development of OS.…”

Section: Discussionmentioning

confidence: 99%

“…miR-605 has been reported to serve roles in non-small cell lung cancer, as cell proliferation and metastasis are inhibited by the downregulation of miR-605 ( 18 ). In prostate cancer, miR-605 is downregulated, which promotes the proliferation and invasion of prostate cancer by targeting engrailed-2 (EN2) ( 19 ). As miR-605 is downregulated in OS, it may be involved in the progression of OS ( 20 ).…”

Section: Introductionmentioning

confidence: 99%

Downregulation of microRNA‑605 indicates poor prognosis and promotes the progression of osteosarcoma

2020

Oncol Lett

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Osteosarcoma (OS) is a type of primary bone tumor, which is one of the leading causes of cancer-related death. MicroRNA (miR)-605 has been demonstrated to act as a prognostic biomarker and therapeutic target in various cancers, such as breast cancer and non-small cell lung cancer, but its function in OS remains unclear. The aim of the present study was to investigate the prognostic value of miR-605 in patients with OS by evaluating its expression levels and to explore the biological function of miR-605 in OS progression. For this purpose, tumor tissues and adjacent normal tissues were collected from OS patients, and the expression of miR-605 in the collected tissues and OS MG63, U2OS, HOS, and SAOS-2 cell lines was detected by quantitative real-time PCR. The prognostic value of miR-605 was evaluated by Kaplan-Meier survival curves and Cox regression analysis. The effects of miR-605 on OS cell proliferation, migration and invasion were analyzed by the CCK-8 and transwell assays, respectively. The results of the present study revealed that miR-605 was significantly downregulated in OS tissues compared with adjacent normal tissues, which was associated with the clinical stage and distant metastasis of patients. Additionally, the downregulation of miR-605 predicted the poor prognosis of patients with OS and served as an independent prognostic indicator. The downregulation of miR-605 enhanced cell proliferation, migration, and invasion of OS cells, which suggested that miR-605 may be involved in the progression of OS. The findings of the present study provide a new therapeutic target for the treatment of patients with OS.

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Down-regulation of miR-605 promotes the proliferation and invasion of prostate cancer cells by up-regulating EN2

Cited by 31 publications

References 22 publications

Pinostilbene Hydrate Suppresses Human Oral Cancer Cell Metastasis by Downregulation of Matrix Metalloproteinase-2 Through the Mitogen-Activated Protein Kinase Signaling Pathway

Pinostilbene Hydrate Suppresses Human Oral Cancer Cell Metastasis by Downregulation of Matrix Metalloproteinase-2 Through the Mitogen-Activated Protein Kinase Signaling Pathway

The role of microRNAs in prostate cancer migration, invasion, and metastasis

Downregulation of microRNA‑605 indicates poor prognosis and promotes the progression of osteosarcoma

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