Down-regulation of pro-necroptotic molecules blunts necroptosis during myogenesis

Taeyeon, Kim; Kang, Ju-Hui; Lee, Se-Bin; Kang, Tae-Bong; Lee, Kwang-Ho

doi:10.1016/j.bbrc.2021.04.004

Cited by 4 publications

(2 citation statements)

References 32 publications

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“…As shown in figure 1, we evaluated how necroptosis functions in C2C12 cells. Because few studies have examined p-MLKL expression by western blotting in C2C12 cells (20), this result showed that C2C12 cells are useful to study cell death mechanisms. Accordingly, few studies have focused on the relationships between muscle and necroptosis.…”

Section: Discussionmentioning

confidence: 97%

Calcium is Involved in Necroptosis Through the Expression of Phospho-MLKL in Skeletal Muscle Cell in Mice

Yashiro¹,

Matsuzaka²

2023

Muscle Ligaments and Tendons J

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Necroptosis is programmed cell death similar to necrosis, which is mediated by meticulous protein interactions such as those in apoptosis. Essentially, because it is a reaction to inflammation, necroptosis functions similarly to apoptosis and it is thought that necroptosis is an alternative cell death pathway when apoptosis cannot function for some reason. However, the research history of necroptosis is brief, and although there are various studies, many aspects are unknown. Here, we found that calcium was an essential factor for necroptosis in a muscle cell line. After verifying necroptosis was functional in mouse skeletal muscle cell line C2C12, we found suppression of necroptosis by controlling the amount of calcium in the culture medium. Furthermore, the reduced necroptosis was rescued by adding calcium to the culture medium. However, the recovery did not occur when magnesium or sodium was added to the medium. Next, we suppressed certain calcium transporters using inhibitors. Necroptosis was inhibited by reducing the amount of intracellular calcium. These results showed that necroptosis was controlled by the amount of calcium. This finding may facilitate development of new treatment methods for various muscle diseases that involve cell death.

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Section: Discussionmentioning

confidence: 97%

Calcium is Involved in Necroptosis Through the Expression of Phospho-MLKL in Skeletal Muscle Cell in Mice

Yashiro¹,

Matsuzaka²

2023

Muscle Ligaments and Tendons J

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“…The total protein in RBL-2H3 mast cells was extracted using RIPA lysis buffer containing phosphatase and protease inhibitors [ 20 ]. The extracted proteins were quantified using the Pierce BCA Protein Assay Kit.…”

Section: Methodsmentioning

confidence: 99%

Lactobacillus gasseri MG4247 and Lacticaseibacillus paracasei MG4272 and MG4577 Modulate Allergic Inflammatory Response in RAW 264.7 and RBL-2H3 cells

Lee

Kang

Jung

et al. 2022

Probiotics & Antimicro. Prot.

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Allergic inflammation refers to a hyperimmune reaction that causes hypersensitivity responses such as hives, itchiness, runny nose, and cough due to specific allergens. Allergic diseases are known to be influenced by the diversity and distribution of intestinal microbiota, and Lactobacill is known to relieve allergic symptoms by modulating cytokines secreted by T helper type 1 (Th1)/Th2 cells. This study was designed to investigate the effects of Lactobacillus gasseri MG4247 and Lacticaseibacillus paracasei MG4272, MG4577, and MG4657 on levels of pro-inflammatory cytokines and proteins associated with allergic symptoms in RAW 264.7 macrophages, and RBL-2H3 mast cells, as well as their probiotic properties. MG4247, MG4272, and MG4577 significantly reduced tumor necrosis factor-α and interleukin (IL)-6 levels in LPS-induced RAW 264.7 macrophages, and markedly decreased IL-4, IL-5, and IL-13 levels and STAT6 phosphorylation in DNP-IgE/HSA sensitized RBL-2H3 mast cells. Furthermore, MG4247, MG4272, and MG4577 tolerated the acidic condition with pepsin and basic condition with bile salt, and showed a high adhesion rate (≥ 73.9%). In safety evaluation, MG4247, MG4272, and MG4577 showed no hemolytic or bile salt hydrolase activity and no cytotoxicity to HT-29 cells (≥ 96.7%). Hence, MG4272, MG4272, and MG4577 can be used as candidate probiotic strains to relieve cytokines associated with allergic inflammation. Supplementary Information The online version contains supplementary material available at 10.1007/s12602-022-09950-4.

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The emerging roles of necroptosis in skeletal muscle health and disease

Qaisar

2024

Pflugers Arch - Eur J Physiol

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Down-regulation of pro-necroptotic molecules blunts necroptosis during myogenesis

Cited by 4 publications

References 32 publications

Calcium is Involved in Necroptosis Through the Expression of Phospho-MLKL in Skeletal Muscle Cell in Mice

Calcium is Involved in Necroptosis Through the Expression of Phospho-MLKL in Skeletal Muscle Cell in Mice

Lactobacillus gasseri MG4247 and Lacticaseibacillus paracasei MG4272 and MG4577 Modulate Allergic Inflammatory Response in RAW 264.7 and RBL-2H3 cells

The emerging roles of necroptosis in skeletal muscle health and disease

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