Down-regulation of Sox10 with specific small interfering RNA promotes transdifferentiation of Schwannoma cells into myofibroblasts

Roh, Jiwon; Cho, Eun Ah; Seong, Ikjoo; Limb, Jin Kyung; Lee, Sanghyuk; Han, Sung‐Jun; Kim, Jaesang

doi:10.1111/j.1432-0436.2006.00084.x

Cited by 21 publications

(16 citation statements)

References 45 publications

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“…The present study demonstrated that ST88-14 expresses many of the molecules predominantly present in non-myelinating SC (e.g., GFAP, NGF-R, CD44) and may be useful in mimicking M. leprae natural infection (Roh et al 2006). Surprisingly, ST88-14 also expressed very low levels of MBP, suggesting that, although myelin was not produced, the gene was transcribed in this lineage.…”

Section: Discussionmentioning

confidence: 69%

Morphological and functional characterizations of Schwann cells stimulated with Mycobacterium leprae

Silva¹,

Silva²,

Baruque³

et al. 2008

Mem. Inst. Oswaldo Cruz

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Section: Discussionmentioning

confidence: 69%

Morphological and functional characterizations of Schwann cells stimulated with Mycobacterium leprae

Silva¹,

Silva²,

Baruque³

et al. 2008

Mem. Inst. Oswaldo Cruz

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“…CG4 oligodendrocytes were transfected using the Amaxa Nucleofection system and either Ambion control or Sox10 siRNA as reported previously (31). S16 Schwann cells were transfected with the following siRNAs using the Amaxa system: Ambion control siRNAs 1 (siControl, catalog number AM4611) and 2 (siControl number 2, catalog number AM4613), Ambion Sox10 siRNA (siSox10 1, catalog number s131239), Qiagen control siRNA (siControl 3, catalog number 1027280), Sox10 siRNA (siSox10 3, catalog number S101429351), and a Proligo Sox10 siRNA previously published (54). For the HTG assay, 48 h posttransfection with the Ambion siRNAs the cells were washed with phosphatebuffered saline (PBS), lysed with HTG lysis buffer, boiled for 5 min, frozen, and shipped to HTG for processing.…”

Section: Methodsmentioning

confidence: 99%

Developmental Regulation of MicroRNA Expression in Schwann Cells

Gokey

Srinivasan

Lopez‐Anido

et al. 2012

Molecular and Cellular Biology

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Schwann cell differentiation and subsequent myelination of the peripheral nervous system require the action of several transcription factors, including Sox10, which is vital at multiple stages of development. The transition from immature to myelinating Schwann cell is also regulated posttranscriptionally and depends upon Dicer-mediated processing of microRNAs (miRNAs). Although specific miRNA targets have begun to be identified, the mechanisms establishing the dynamic regulation of miRNA expression have not been elucidated. We performed expression profiling studies and identified 225 miRNAs differentially expressed during peripheral myelination. A subset of 9 miRNAs is positively regulated by Sox10, including miR-338 which has been implicated in oligodendrocyte maturation. In vivo chromatin immunoprecipitation (ChIP) of sciatic nerve cells revealed a Sox10 binding site upstream of an alternate promoter within the Aatk gene, which hosts miR-338. Sox10 occupied this site in spinal cord ChIP experiments, suggesting a similar regulatory mechanism in oligodendrocytes. Cancer profiling studies have identified clusters of miRNAs that regulate proliferation, termed "oncomirs." In Schwann cells, the expression of many of these proproliferative miRNAs was reduced in the absence of Sox10. Finally, Schwann cells with reduced Sox10 and oncomir expression have an increase in the CDK inhibitor p21 and a concomitant reduction in cell proliferation.

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“…2 An interesting hypothesis explaining SOX10's mechanistic role in myoepithelial cells is that its downregulation induces the transdifferentiation of Schwann cells to myofibroblasts. 3 From the standpoint of embryology, previous work has established that parotid glands originate from the oral ectoderm; 4 however, the embryologic origins of the submandibular and sublingual gland epithelium have not been clearly defined, although ectodermal origins are suggested. 4 In the development of mouse submandibular glands, SOX10 expression has been associated with the presence of epithelial stem/progenitor cells from embryonic day13.5 (E13.5) to adulthood.…”

mentioning

confidence: 99%

SOX10 is a novel marker of acinus and intercalated duct differentiation in salivary gland tumors: a clue to the histogenesis for tumor diagnosis

Mori²,

et al. 2013

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Salivary gland tumors are relatively rare and morphologically diverse and heterogeneous tumors; therefore, histogenesis-based tumor markers are sorely needed to aid in diagnosing and determining the cell type of origin. SRY-related HMG-box 10 (SOX10) protein is a transcription factor known to be crucial in the specification of the neural crest and maintenance of Schwann cells and melanocytes. In addition, positive expression has also been implicated in the major salivary gland. Here, we examined SOX10 expression in various salivary gland tumors to correlate this expression with myoepithelial markers. Overall, 76 malignant and 14 benign tumors were examined. SOX10 expression clearly delineated two distinct subtypes of human salivary gland tumors; acinic cell carcinomas, adenoid cystic carcinomas, epithelial-myoepithelial carcinomas, myoepithelial carcinomas, and pleomorphic adenomas, including the pleomorphic adenoma component of carcinoma, were SOX10 positive, while salivary duct carcinomas, mucoepidermoid carcinomas, an oncocytic carcinoma, Oncocytomas, and Warthin tumors were SOX10 negative. Also, SOX10 was expressed in solid-type or nonspecific morphology salivary gland tumors, but was not expressed in poorly differentiated squamous cell carcinomas. In normal human salivary gland tissue, SOX10 expression was specific to the nuclei of acini and both luminal and abluminal cells of intercalated ducts but not in other sites. Moreover, the murine model suggested that SOX10 continued to be expressed from the developmental stage to adulthood in the acinar and both luminal and abluminal intercalated ducts in the major salivary gland. Thus, SOX10 is a novel marker for diagnosing and understanding the histogenesis of salivary gland tumors.

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Down-regulation of Sox10 with specific small interfering RNA promotes transdifferentiation of Schwannoma cells into myofibroblasts

Cited by 21 publications

References 45 publications

Morphological and functional characterizations of Schwann cells stimulated with Mycobacterium leprae

Morphological and functional characterizations of Schwann cells stimulated with Mycobacterium leprae

Developmental Regulation of MicroRNA Expression in Schwann Cells

SOX10 is a novel marker of acinus and intercalated duct differentiation in salivary gland tumors: a clue to the histogenesis for tumor diagnosis

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