Down-regulation of the Surface Expression of Class I MHC Antigens by Human Cytomegalovirus

Yamashita, Yasuo; Shimokata, Kaoru; Mizuno, Shinichi; Yamaguchi, Hisateru; Nishiyama, Yukihiro

doi:10.1006/viro.1993.1181

Cited by 61 publications

(39 citation statements)

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“…These results are consistent with previous reports (21,54). Control experiments analyzing the surface expression of MHC-I and ICAM-1 on HUVEC as well as fibroblasts gave results which were in agreement with published data (9,18,41,53). The expression of MHC-I was downregulated, whereas that of ICAM-1 was upregulated (Fig.…”

Section: Methodssupporting

confidence: 93%

Upregulation of CD40 Expression on Endothelial Cells Infected with Human Cytomegalovirus

Maisch

Kropff

Sinzger

et al. 2002

J Virol

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Section: Methodssupporting

confidence: 93%

Upregulation of CD40 Expression on Endothelial Cells Infected with Human Cytomegalovirus

Maisch

Kropff

Sinzger

et al. 2002

J Virol

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“…This mutant lacks a 15 kb spanning genomic region within the unique short region (Us) corresponding to the genes U,,ql-USI5 (Kollert-J6ns et al, 1991;Mockenhaupt et al, 1994;Yamanishi & Rapp, 1979). As reported by several laboratories (Barnes & Grundy, 1992;Beersma et al, 1993;Hengel et al, 1995 ;Warren et al, 1994;Yamashita et al, 1993), fibroblasts infected with AD169 for 72 h express drastically reduced steady state levels of MHC class I complexes as…”

Section: Resultsmentioning

confidence: 91%

“…As demonstrated in Fig. i (a controls (Beersma eta]., 1993;Yamashita et al, 1993;Hengel et aI., 1995). The addition of PAA (Fig.…”

Section: Hcmv Infection Induces Expression Of the Tap1/2 Heterodimer mentioning

confidence: 81%

“…During HCMV infection, the assembly of MHC class l complexes is downregulated and antigen presentation is abolished (Beersma et al,I993;Warren eta]., 1994; Yamashita et al, 1993;Hengel et al, 1995). This immuno-evasive action is under the control of HCMV early genes.…”

Section: Discussionmentioning

confidence: 99%

“…In MCMV-infected cells, correctly assembled peptide-charged MHC class I complexes are retained in the ER/cis-Golgi compartment by an early gene function leading to abrogation of cytotoxic T lymphocyte recognition (Del Valet al, 1992). In contrast to MCMV, in HCMV-infected fibroblasts MHC class I assembly is reduced and degradation of class I molecules is enhanced (Beersma et aI., 1993;Warren et aI., 1994;Yamashita et al, 1993). The HCMV USll encoded 32 kDa glycoprotein, which is sufficient for MHC class I downregulation (Jones eta]., 1995), misdirects MHC class I heavy chains from the ER to the cytosol where they are degraded by the proteasome (Wiertz et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Human Cytomegalovirus Inhibits Peptide Translocation into the Endoplasmic Reticulum for MHC Class I Assembly

Hengel

Flohr

Hämmerling

et al. 1996

Journal of General Virology

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Human cytomegalovirus (HCMV) genes expressed in the early phase of infection mediate the destabilization of nascent major histocompatibility complex (MHC) class I molecules in infected cells and thus prevent antigen presentation to CD8 ÷ T lymphocytes. We report that HCMV genes interfere with the MHC class I pathway of antigen presentation by at least two mechanisms. Firstly, permissive infection of fibroblasts is characterized by a continuous decline in the capacity to translocate peptides from the cytosol into the endoplasmic reticulum (ER) by the transporter associated with antigen processing (TAP). Inactivation of peptide transport is operative despite augmented TAP expression during HCMV infection. Secondly, TAP molecules fail to associate with MHC class I heavy chains indicating that HCMV early gene expression also interferes with MHC class I maturation. A temperature-sensitive mutant of HCMV, ts9, which lacks 1 5 kb of DNA encoding the genes US1-US15 of HCMV, had lost the capacity to interfere with MHC class I assembly and to inhibit the peptide translocation function of TAP. One of the genes deleted in ts9, US 1 1, which was reported to downregulate the expression of MHC class I molecules, does not affect peptide transport by TAP. Therefore, we conclude that HCMV encodes at least two early gene functions that interfere with the MHC class I antigen presentation pathway.

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The Role of CD8 T Cells in the Control of Infectious Disease and Malignancies

Hsu¹

2010

Topley &Amp; Wilson's Microbiology and Microbial Infections

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Down-regulation of the Surface Expression of Class I MHC Antigens by Human Cytomegalovirus

Cited by 61 publications

References 0 publications

Upregulation of CD40 Expression on Endothelial Cells Infected with Human Cytomegalovirus

Upregulation of CD40 Expression on Endothelial Cells Infected with Human Cytomegalovirus

Human Cytomegalovirus Inhibits Peptide Translocation into the Endoplasmic Reticulum for MHC Class I Assembly

The Role of CD8 T Cells in the Control of Infectious Disease and Malignancies

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