Downregulation of AdipoR1 is Associated with Increased Circulating Adiponectin Levels in Serbian Chronic Kidney Disease Patients

Abstract

SummaryBackgroundSince the rise in plasma adiponectin levels in chronic kidney disease (CKD) patients has not yet been elucidated, we sought to investigate if patients on hemodialysis (HD) have altered expression of adiponectin receptors in peripheral blood mononuclear cells (PBMCs) compared to healthy subjects.MethodsThis study included 31 patients with chronic kidney disease on HD and 33 healthy subjects (CG). Circulating adiponectin levels were measured by ELISA while AdipoR1 and AdipoR2 mRNA levels in PBMCs were determined by real-time PCR.ResultsPlasma adiponectin levels were significantly higher in patients compared to control group (P=0.036). After adjustment for age, BMI and creatinine, this difference became even more significant (P=0.004). In both groups adiponectin correlated with creatinine (CG: r=-0.472, P=0.006; HD: r=-0.375, P=0.038), triglycerides (CG: r=- 0.490, P=0.004; HD: r=-0.488, P=0.005), insulin (CG: r=-0.386, P=0.038; HD: r=-0.506, P=0.012) and high density lipoprotein cholesterol (HDL-C) (CG: r=-0.672, P<0.001; HD: r=-0.584, P=0.001). Significantly lower expression of PBMCs AdipoR1 mRNA was found in patients compared to CG (P=0.034), while AdipoR2 mRNA levels were similarly expressed in PBMCs in both groups.ConclusionsComplex pathological processes in CKD cause downregulation of AdipoR1 which could ultimately influence AdipoR1 protein levels leading to a state of ≫adiponectin resistance≪.

Alterations of HDL Particles in Children with End-Stage Renal Disease

“…In CKD patients, higher adiponectin concentrations which are usually more than three times higher compared with healthy individuals are associated with poor outcomes and increased mortality (21). Result of our study confirmed previous founding (19, 22) that the high levels of adiponectin decline after renal transplantation ( Table II ). Also, we found significant positive correlation between adiponectin concentration and presence of smaller HDL 3b particles ( Table III ), so we could speculate that even adiponectin is presumed to possess antiatherogenic properties in the uncommon uremic milieu, higher adiponectin levels are associated with increased risk for cardiovascular disease development.…”

“…Even it is good known that adiponectin has cardio protective and anti-inflammatory effects, the role of this adipocytokine in patients with CKD are still unclear (19). Results of previous studies have confirmed a positive association between adiponectin concentration and progression of CKD (19).…”

“…Even it is good known that adiponectin has cardio protective and anti-inflammatory effects, the role of this adipocytokine in patients with CKD are still unclear (19). Results of previous studies have confirmed a positive association between adiponectin concentration and progression of CKD (19). The main reasons for high adiponectin concentrations in uremic patients are reduced adiponectin clearance by the impaired kidneys and changes in adiponectin binding for their receptors (20).…”

Upregulation of the adipokine genes ADIPOR1 and SPP1 is related to poor survival outcomes in colorectal cancer

“…Two possible explanations could be postulated. One, the low plasma adiponectin level, which could have an anti‐protective effect on CRC prognosis, might have compensatorily resulted in the upregulation of the adiponectin receptor mRNA level in the CRC tissue . Second, the increased ADIPOR1 mRNA expression level implies increased adiponectin bioavailability, which has a pro‐angiogenesis effect, resulting in the progression of CRC and poor survival.…”

Myo-Inositol Oxygenase as a Novel Marker in the Diagnosis of Acute Kidney Injury

“…So in our study, the cause of high level of MIOX in AKI patients may be related to increased to ROS. Because it is also known that there is an association with oxidative stress and AKI (19, 20).…”