Downregulation of CuZn-superoxide dismutase contributes to β-adrenergic receptor-mediated oxidative stress in the heart

Srivastava, Sanjay; Chandrasekar, Bysani; Gu, Yu; Luo, Jianzhu; Hamid, Tariq; Hill, Bradford G.; Prabhu, Sumanth D.

doi:10.1016/j.cardiores.2007.02.016

Cited by 109 publications

(86 citation statements)

References 33 publications

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“…The more severe hypertension and LV remodeling in the CIH-vehicle group at 6 months could be ascribed because of a variety of mechanisms initiated by exposure to CIH. 4,11,12,17,26,27 ROS and L-Ca 2ϩ channel mechanisms might not necessarily be the only standing mechanisms for the exaggerated CV dysfunction at 6 months in the SHR, although they appear to be critical for the initiation and progression of the developmental programming of long-term CV morbidity after CIH in neonatal SHRs.…”

Section: Discussionmentioning

confidence: 99%

“…26,27 Imaging was performed under light anesthesia with ketamine (22 mg/kg, IM) and xylazine (2.7 mg/kg, IM), using a Toshiba 380 PowerVision machine and a pediatric broad-band transducer operating at 10-MHz frequency and 120-Hz frame rate. At the age of 6 to 7 months, the following variables were measured: LV end-diastolic and end-systolic diameter (LVEDD and LVESD), anterior wall thickness (AWT) and posterior wall thickness (PWT) at end diastole, and ejection time from the aortic Doppler trace.…”

Section: Echocardiographic Assessment Of LV Functionmentioning

confidence: 99%

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Postnatal Intermittent Hypoxia and Developmental Programming of Hypertension in Spontaneously Hypertensive Rats

Soukhova-O'Hare

Ortines

et al. 2008

Hypertension

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Abstract-Obstructive and central apneas during sleep are associated with chronic intermittent hypoxia (CIH) and increased cardiovascular morbidity. Spontaneously hypertensive rats exposed to CIH during postnatal days 4 to 30 develop exaggerated hypertension as adults. We hypothesized that reactive oxygen species and altered L-Ca 2ϩ channel activity may underlie the postnatal programming of exaggerated blood pressure and cardiac remodeling. Newborn male spontaneously hypertensive rats were exposed to CIH (10% and 21% O 2 alternating every 90 seconds, 12 h/d, for postnatal days 4 to 30) or normoxia (room air). In each condition, spontaneously hypertensive rats received daily (SC) 1 of 3 treatments: L-calcium channel blocker nifedipine (5 mg/kg), superoxide dismutase mimetic MnTMPyP pentachloride (10 mg/kg), or vehicle (polyethylene glycol). Blood pressure was evaluated monthly for 6 months after birth, and echocardiographic assessments were conducted at 6 months of age. CIH vehicle-treated rats presented higher systolic blood pressure (187Ϯ5 mm Hg) as compared with normoxic vehicle treated controls (163Ϯ2 mm Hg; PϽ0.001). Postnatal CIH elicited marked increases in left ventricular wall thickness in a pattern of concentric hypertrophy with augmented systolic contractility. The treatment with nifedipine in the CIH group attenuated blood pressure (159Ϯ2 mm Hg; PϽ0.001) and normalized left ventricular wall thickness and systolic function, whereas the treatment with SOD mimetic decreased blood pressure (165Ϯ2 mm Hg; PϽ0.001) and reduced left ventricular wall thickness without changes in the systolic function. We conclude that Ca 2ϩ and reactive oxygen species-mediated signaling during intermittent hypoxia are critical mechanisms underlying postnatal programming of an increased severity of hypertension and hypertrophic cardiac remodeling in a genetically susceptible rodent model. Key Words: intermittent hypoxia Ⅲ calcium channels Ⅲ oxidative stress Ⅲ sleep apnea Ⅲ hypertension I ntermittent hypoxia (IH) during sleep is one of the characteristics of sleep apnea that is associated with substantial cardiovascular (CV) morbidity, such as hypertension and heart failure. Sleep-disordered breathing with apneic episodes and intermittent hypoxemia are frequently encountered in young children 1 ; however, the potential consequences of postnatal IH on adult CV dysfunction are not clear. There is accumulating evidence that IH is associated with an increased oxidative stress [2][3][4][5][6] and that redox signaling during this condition has been extensively implicated in CV pathophysiology, including some forms of hypertension and cardiac function. 2,7-9 Perinatal L-arginine and antioxidant supplements were shown to reduce blood pressure (BP) in adult spontaneously hypertensive rats (SHRs), suggesting a critical role of oxidative status during perinatal development in programming BP later in life. 10 In previous work from our laboratory, we demonstrated that SHRs exposed for the first 30 days of postnatal life to a chronic intermitt...

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Section: Discussionmentioning

confidence: 99%

Section: Echocardiographic Assessment Of LV Functionmentioning

confidence: 99%

Postnatal Intermittent Hypoxia and Developmental Programming of Hypertension in Spontaneously Hypertensive Rats

Soukhova-O'Hare

Ortines

et al. 2008

Hypertension

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“…The following measurements were made during recording or off-line from stored data on video tape after frame-digitization: 1) LV anteroposterior diameter (D, mm), anterior and posterior wall thickness (AWT and PWT, mm, respectively) and SAX cross-sectional area (A, mm 2 ) at end-diastole (ED) and end-systole (ES) [21,22]. LV hypertrophy was additionally assessed by: 1) the relative wall thickness (RWT = EDAWT + EDPWT)/EDD and 2) LV mass (mg): [(EDD+EDAWT+EDPWT) 3 …”

Section: Diabetic Cardiomyopathy In Ove26 Micementioning

confidence: 99%

Diabetic Cardiomyopathy in OVE26 Mice Shows Mitochondrial ROS Production and Divergence Between In Vivo and In Vitro Contractility

Song

Du²,

Prabhu³

et al. 2007

Rev Diabet Stud

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■ AbstractMany diabetic patients suffer from a cardiomyopathy that cannot be explained solely by poor coronary perfusion. This cardiomyopathy may be due to either organ-based damage like fibrosis, or to direct damage to cardiomyocytes. Mitochondrial-derived reactive oxygen species (ROS) have been proposed to contribute to this cardiomyopathy. To address these questions, we used the OVE26 mouse model of severe type 1 diabetes to measure contractility in isolated cardiomyocytes by edge detection and in vivo with echocardiography. We also assessed the source of ROS generation using both a general and a mitochondrial specific indicator. When contractility was assayed in freshly isolated myocytes, contraction was much stronger in control myocytes. However, contractility of normal myocytes became weaker during 24 hours of in vitro culture. In contrast, contractility of diabetic OVE26 myocytes remains stable during culture. Echocardiography revealed normal or hyperdynamic function in OVE26 hearts under basal conditions but with a sharply reduced response to isoproterenol, a β-adrenergic agonist. For ROS generation, we found that ROS production in diabetic myocytes was elevated after exposure to either high glucose or angiotensin II (AngII). Superoxide detection with the mitochondrial sensor MitoSOX Red confirmed that mitochondria are a major source of ROS generation in diabetic myocytes. These results show that contractile deficits in OVE26 diabetic hearts are due primarily to cardiomyocyte impairment and that ROS from mitochondria are a cause of that impairment.

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“…Cardiotoxicity mediated by ISO due to stimulation of b 1 e adrenergic receptor and this stimulation not only rapidly generate ROS, but also depresses the myocardial endogenous antioxidant system leading to cause myocardial IRI. 30,31 Depletion in the level of myocardial endogenous antioxidants develops a divergence of mitochondrial function. It has already reported that the decrease in the antioxidant level resulted in a diminished hunt of free radical in the ISOeinduced myocardial damage in rats.…”

Section: Resultsmentioning

confidence: 99%

Putative antioxidant property of sesame oil in an oxidative stress model of myocardial injury

Saleem

Chetty²,

Kavimani³

2013

Journal of Cardiovascular Disease Research

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a b s t r a c tBackground: Sesame oil is a potent antioxidant dietary source for human health. Oxidative stress through generation of free radicals damages the myocardium in different experimental condition. The present research was designed to evaluate the antioxidant property of chronic oral administration of sesame oil against isoproterenol induced myocardial injury. Methods and results: Male Wistar albino rats were randomly divided into five groups (n ¼ 6) and treated as per treatment protocol with two different doses of sesame oil (5 and 10 ml/kg b.w.) orally for thirty days. At the end of the treatment all the rats (except control rats) were administered with isoproterenol (85 mg/kg) two consecutive days and subjected to biochemical and histopathological estimation. Isoproterenol (group ISO) induced the oxidative myocardial damage via alteration in the endogenous antioxidant enzymes and myocardial marker enzymes. Sesame oil in both the dose (group S1 and S2) shows protective mechanism via decreasing thiobarbituric acid reactive substance (TBARS) and enhancing the endogenous antioxidant enzymes (reduced glutathione (GSH), superoxide dismutase (SOD) and Catalase). Sesame oil also increased the lactate dehydrogenase (LDH), creatine kinase (CK) and aspartate transaminase (AST) as a myocardial marker enzyme in heart homogenate. As histologically evident isoproterenol induced myocardial injury was well preserved by the chronic administration of sesame oil. The protective role of sesame oil was compared with the reference standard a-tocopherol (group S3) also showing the similar effect. Conclusion: From this finding it has been concluded that chronic administration of sesame oil offers cardio protective action via putative antioxidant property.

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Downregulation of CuZn-superoxide dismutase contributes to β-adrenergic receptor-mediated oxidative stress in the heart

Abstract: Sustained beta-AR stimulation transcriptionally downregulates CuZn-SOD in myocardium via the beta1-AR, thereby contributing to beta-AR-mediated oxidative stress.

Cited by 109 publications

References 33 publications

Postnatal Intermittent Hypoxia and Developmental Programming of Hypertension in Spontaneously Hypertensive Rats

Postnatal Intermittent Hypoxia and Developmental Programming of Hypertension in Spontaneously Hypertensive Rats

Diabetic Cardiomyopathy in OVE26 Mice Shows Mitochondrial ROS Production and Divergence Between In Vivo and In Vitro Contractility

Putative antioxidant property of sesame oil in an oxidative stress model of myocardial injury

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